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Conference Paper: The cytoprotective action of metronidazole in rats stomachs

TitleThe cytoprotective action of metronidazole in rats stomachs
Authors
Issue Date1995
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Digestive Disease Week and the 95th Annual Meeting of the American Gastroenterological Association, San Diego, CA, 14–17 May 1995. In Gastroenterology, 1995, v. 108 n. 4 S1, p. A71 How to Cite?
AbstractWe demonstrated that amoxiciUin imparted gastric cytoprotection when ~dministered before the fat stomachs were challenged by ethanol (J. Gastroenterol. Hepatol. 1994, 9:514-8). This study was performed to evaluate if metronidazole has a similar protective property. Sprague-Dawiey rats (230-250 g) were used for these experiments. These animals were fasted for 24 hrs before metronidazole (5 or 10 mg/kg) or its vehicle (controls) administered via the oral or intraperitoneal route before ethanol (40%, v/v 10 ml/kg) or ~domethaein (30 mg/kg) ingestion. The severity ofmucosal lesions was assessed by measuring the lesion areas and the intramucosal mucus thickness was determined by the histochemical method. An ex-vivo gastric chamber was used to measure the changes in basal mucosal blood flow (the laser-Doppler method) with the same doses of metronidazole. Finally, the direct cytoprotective effect of the drug was assessed by the viability and lactate dehydrogenase release from an o isolated gland preparation after ethanol (10'A, v/v) incubation. Metronidazole pretreatment by either route markedly prevented ethanol-induced gastric mucosal injury by 4-5 folds. Similar protection was observed in the indomethacin-treated animals, indicating that the antiulcer action was unlikely to be prostaglandin-mediated. The drug did not affect the gastric intramucosal mucus thickness or the basal mucosal blood flow. Metronidazole preincubation (10 "~ or 10 .4 M) significantly attenuated the damaging effect of ethanol on the viability of isolated glands (from 80% to 92%), and there was also a parallel significant reduction (3 folds) in lactate dehydrogenase release from the gland preparation. It is concluded that metronidazole prevents ethanol-induced mucosal lesions by a direct cytoprotective action on the gastric glandular mucosa.
Persistent Identifierhttp://hdl.handle.net/10722/78433
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorCho, CHen_HK
dc.contributor.authorKaan, SKen_HK
dc.contributor.authorKo, JKSen_HK
dc.contributor.authorChing, CKen_HK
dc.contributor.authorLam, KSLen_HK
dc.date.accessioned2010-09-06T07:42:50Z-
dc.date.available2010-09-06T07:42:50Z-
dc.date.issued1995en_HK
dc.identifier.citationDigestive Disease Week and the 95th Annual Meeting of the American Gastroenterological Association, San Diego, CA, 14–17 May 1995. In Gastroenterology, 1995, v. 108 n. 4 S1, p. A71en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78433-
dc.description.abstractWe demonstrated that amoxiciUin imparted gastric cytoprotection when ~dministered before the fat stomachs were challenged by ethanol (J. Gastroenterol. Hepatol. 1994, 9:514-8). This study was performed to evaluate if metronidazole has a similar protective property. Sprague-Dawiey rats (230-250 g) were used for these experiments. These animals were fasted for 24 hrs before metronidazole (5 or 10 mg/kg) or its vehicle (controls) administered via the oral or intraperitoneal route before ethanol (40%, v/v 10 ml/kg) or ~domethaein (30 mg/kg) ingestion. The severity ofmucosal lesions was assessed by measuring the lesion areas and the intramucosal mucus thickness was determined by the histochemical method. An ex-vivo gastric chamber was used to measure the changes in basal mucosal blood flow (the laser-Doppler method) with the same doses of metronidazole. Finally, the direct cytoprotective effect of the drug was assessed by the viability and lactate dehydrogenase release from an o isolated gland preparation after ethanol (10'A, v/v) incubation. Metronidazole pretreatment by either route markedly prevented ethanol-induced gastric mucosal injury by 4-5 folds. Similar protection was observed in the indomethacin-treated animals, indicating that the antiulcer action was unlikely to be prostaglandin-mediated. The drug did not affect the gastric intramucosal mucus thickness or the basal mucosal blood flow. Metronidazole preincubation (10 "~ or 10 .4 M) significantly attenuated the damaging effect of ethanol on the viability of isolated glands (from 80% to 92%), and there was also a parallel significant reduction (3 folds) in lactate dehydrogenase release from the gland preparation. It is concluded that metronidazole prevents ethanol-induced mucosal lesions by a direct cytoprotective action on the gastric glandular mucosa.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.titleThe cytoprotective action of metronidazole in rats stomachsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=108&spage=A71&epage=&date=1995&atitle=The+cytoprotective+action+of+metronidazole+in+rat+stomachsen_HK
dc.identifier.emailCho, CH: chcho@hkusua.hku.hken_HK
dc.identifier.emailKo, JKS: jksko@hkucc.hku.hken_HK
dc.identifier.emailChing, CK: chi_kong_ching@hotmail.comen_HK
dc.identifier.emailLam, KSL: ksllam@hku.hken_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0016-5085(95)22949-3-
dc.identifier.hkuros8544en_HK

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