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Article: Chronic treatment with growth hormone stimulates adiponectin gene expression in 3T3-L1 adipocytes

TitleChronic treatment with growth hormone stimulates adiponectin gene expression in 3T3-L1 adipocytes
Authors
KeywordsAdipocyte
Adipokine
Adiponectin
Growth hormone
Insulin sensitivity
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 2004, v. 572 n. 1-3, p. 129-134 How to Cite?
AbstractGrowth hormone (GH) is an important regulator of adiposity and systemic energy metabolism. Here, we have investigated the effects of GH on production of adiponectin, an anti-diabetic and anti-atherogenic hormone secreted exclusively from adipocytes. Analysis using real time quantitative PCR revealed that GH significantly increased adiponectin gene expression in a dose-dependent manner. Time course study showed that the expression of adiponectin gene started to increase only after 30 h of GH treatment (10-8 M), suggesting it to be a chronic effect. GH-mediated induction of adiponectin gene expression was completely blocked by treatment with the Janus kinase2 (JAK2) inhibitor AG490 and the P38 mitogen activated protein (MAP) kinase inhibitor SB203580, while the specific inhibitors of phosphatidylinositol-3-kinase (LY294002) and p70S6 kinase (rapamycin) moderately enhanced GHs effect. Co-incubation of adipocytes with GH and the PPARγ agonist rosiglitazone produced additive effects on induction of adiponectin gene expression. These results collectively suggest that GH increases adiponectin gene expression through the JAK2-P38 MAP kinase pathway, and that elevation of adiponectin production might represent a novel mechanism by which GH regulates systemic energy metabolism and insulin sensitivity. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Persistent Identifierhttp://hdl.handle.net/10722/78345
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Aen_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorXu, JYen_HK
dc.contributor.authorCooper, GJSen_HK
dc.contributor.authorLam, KSLen_HK
dc.date.accessioned2010-09-06T07:41:51Z-
dc.date.available2010-09-06T07:41:51Z-
dc.date.issued2004en_HK
dc.identifier.citationFebs Letters, 2004, v. 572 n. 1-3, p. 129-134en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78345-
dc.description.abstractGrowth hormone (GH) is an important regulator of adiposity and systemic energy metabolism. Here, we have investigated the effects of GH on production of adiponectin, an anti-diabetic and anti-atherogenic hormone secreted exclusively from adipocytes. Analysis using real time quantitative PCR revealed that GH significantly increased adiponectin gene expression in a dose-dependent manner. Time course study showed that the expression of adiponectin gene started to increase only after 30 h of GH treatment (10-8 M), suggesting it to be a chronic effect. GH-mediated induction of adiponectin gene expression was completely blocked by treatment with the Janus kinase2 (JAK2) inhibitor AG490 and the P38 mitogen activated protein (MAP) kinase inhibitor SB203580, while the specific inhibitors of phosphatidylinositol-3-kinase (LY294002) and p70S6 kinase (rapamycin) moderately enhanced GHs effect. Co-incubation of adipocytes with GH and the PPARγ agonist rosiglitazone produced additive effects on induction of adiponectin gene expression. These results collectively suggest that GH increases adiponectin gene expression through the JAK2-P38 MAP kinase pathway, and that elevation of adiponectin production might represent a novel mechanism by which GH regulates systemic energy metabolism and insulin sensitivity. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectAdipocyteen_HK
dc.subjectAdipokineen_HK
dc.subjectAdiponectinen_HK
dc.subjectGrowth hormoneen_HK
dc.subjectInsulin sensitivityen_HK
dc.titleChronic treatment with growth hormone stimulates adiponectin gene expression in 3T3-L1 adipocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=572&issue=1-3&spage=129&epage=34&date=2004&atitle=Chronic+treatment+with+growth+hormone+stimulates+adiponectin+gene+expression+in+3T3-L1+adipocytesen_HK
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailLam, KSL: ksllam@hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.febslet.2004.07.020en_HK
dc.identifier.pmid15304336-
dc.identifier.scopuseid_2-s2.0-4143096055en_HK
dc.identifier.hkuros98208en_HK
dc.identifier.hkuros120059-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4143096055&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume572en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage129en_HK
dc.identifier.epage134en_HK
dc.identifier.isiWOS:000223519300024-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridWong, LC=25123484000en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridXu, JY=8947805200en_HK
dc.identifier.scopusauthoridCooper, GJS=7402355946en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK

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