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Article: Glycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophages

TitleGlycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophages
Authors
Issue Date2004
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosis
Citation
Atherosclerosis, 2004, v. 177 n. 2, p. 313-320 How to Cite?
AbstractLow-density lipoprotein (LDL) in patients with diabetes is subject to modification by both oxidation and glycation. In contrast to oxidized LDL, the biological effects of glycoxidized LDL have not been well characterised. In this study, the effects of oxidized, glycated, glycoxidized and oxidized LDL on scavenger receptor gene expressions, and the induction of oxidized LDL uptake and cholesteryl ester accumulation in THP-1 macrophages were compared. Modified LDL was incubated with THP-1 macrophages. Gene expression of scavenger receptor class A (SR-A), CD36 and scavenger receptor class B type I (SR-BI) was determined by quantitative reverse transcriptase PCR (RT-PCR). Glycoxidized LDL was able to significantly induce SR-A and CD36 expression by 3- and 4.5-fold, respectively, in macrophages whereas SR-BI expression was suppressed by glycoxidized LDL, glycated LDL and oxidized LDL. Incubation with glycoxidized LDL enhanced the uptake of DiI-labeled oxidized LDL by macrophages to a greater extent than that of glycated LDL or oxidized LDL. Glycoxidized LDL also induced a significant degree of intracellular cholesteryl ester accumulation. Taken together, our results would suggest that glycoxidized LDL might be an important candidate in the initiation of foam cell formation and might play a significant role in the pathogenesis of atherosclerosis in diabetes mellitus. © 2004 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/77861
ISSN
2015 Impact Factor: 3.942
2015 SCImago Journal Rankings: 1.819
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, MCWen_HK
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorLam, KSLen_HK
dc.date.accessioned2010-09-06T07:36:33Z-
dc.date.available2010-09-06T07:36:33Z-
dc.date.issued2004en_HK
dc.identifier.citationAtherosclerosis, 2004, v. 177 n. 2, p. 313-320en_HK
dc.identifier.issn0021-9150en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77861-
dc.description.abstractLow-density lipoprotein (LDL) in patients with diabetes is subject to modification by both oxidation and glycation. In contrast to oxidized LDL, the biological effects of glycoxidized LDL have not been well characterised. In this study, the effects of oxidized, glycated, glycoxidized and oxidized LDL on scavenger receptor gene expressions, and the induction of oxidized LDL uptake and cholesteryl ester accumulation in THP-1 macrophages were compared. Modified LDL was incubated with THP-1 macrophages. Gene expression of scavenger receptor class A (SR-A), CD36 and scavenger receptor class B type I (SR-BI) was determined by quantitative reverse transcriptase PCR (RT-PCR). Glycoxidized LDL was able to significantly induce SR-A and CD36 expression by 3- and 4.5-fold, respectively, in macrophages whereas SR-BI expression was suppressed by glycoxidized LDL, glycated LDL and oxidized LDL. Incubation with glycoxidized LDL enhanced the uptake of DiI-labeled oxidized LDL by macrophages to a greater extent than that of glycated LDL or oxidized LDL. Glycoxidized LDL also induced a significant degree of intracellular cholesteryl ester accumulation. Taken together, our results would suggest that glycoxidized LDL might be an important candidate in the initiation of foam cell formation and might play a significant role in the pathogenesis of atherosclerosis in diabetes mellitus. © 2004 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosisen_HK
dc.relation.ispartofAtherosclerosisen_HK
dc.rightsAtherosclerosis. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshAntigens, CD36 - geneticsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshLipoproteins, LDL - physiologyen_HK
dc.subject.meshMacrophages - chemistryen_HK
dc.subject.meshReceptors, Immunologic - geneticsen_HK
dc.subject.meshReceptors, Scavengeren_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshScavenger Receptors, Class Aen_HK
dc.subject.meshScavenger Receptors, Class Ben_HK
dc.titleGlycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophagesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9150&volume=177&issue=2&spage=313&epage=20&date=2004&atitle=Glycoxidized+low-density+lipoprotein+regulates+the+expression+of+scavenger+receptors+in+THP-1+macrophagesen_HK
dc.identifier.emailTan, KCB:kcbtan@hku.hken_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.atherosclerosis.2004.08.003en_HK
dc.identifier.pmid15530905-
dc.identifier.scopuseid_2-s2.0-8644230195en_HK
dc.identifier.hkuros98203en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-8644230195&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume177en_HK
dc.identifier.issue2en_HK
dc.identifier.spage313en_HK
dc.identifier.epage320en_HK
dc.identifier.isiWOS:000225985600012-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridLam, MCW=36879143100en_HK
dc.identifier.scopusauthoridTan, KCB=8082703100en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK

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