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Article: Quantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinoma

TitleQuantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinoma
Authors
KeywordsHepatitis B virus
Hepatocellular carcinoma
Liver
Tumor
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
Journal Of Hepatology, 2006, v. 45 n. 4, p. 553-559 How to Cite?
AbstractBackground/Aims: This study aimed to measure the intrahepatic total hepatitis B virus (HBV) DNA and covalently closed circular DNA (cccDNA) levels in tumor and non-tumor tissues in hepatocellular carcinoma (HCC) patients. Methods: Intrahepatic total HBV DNA and cccDNA were measured in 25 HCC patients (21 hepatitis B surface antigen [HBsAg]-positive and 4 HBsAg-negative) by the Invader® assay. Results: A low level of intrahepatic HBV DNA was detectable in all HBsAg-negative patients. For HBsAg-positive patients, the intrahepatic total HBV DNA levels in the tumor and non-tumor tissues were comparable (P = 0.903). However, the tumor tissues had significantly higher levels of cccDNA (0.35 vs. 0.16 copies/cell, P = 0.030) and higher proportion of intrahepatic HBV DNA in the form of cccDNA (100% vs. 84%, P = 0.004) than the non-tumor tissues. Seventeen out of 21 (81%) tumor tissues had intrahepatic HBV DNA solely in cccDNA form. Analysis of HBV mRNA expression indicated that HBV replication appeared to be lower in the tumor tissues than the non-tumor tissues. Conclusions: Compared to the non-tumor tissues, the levels of HBV replication in the tumor tissues appeared to be lower, and cccDNA was the predominant form of HBV DNA in the tumor tissues. © 2006 European Association for the Study of the Liver.
Persistent Identifierhttp://hdl.handle.net/10722/77712
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorYuen, JCHen_HK
dc.contributor.authorFung, Jen_HK
dc.contributor.authorLai, CLen_HK
dc.date.accessioned2010-09-06T07:34:54Z-
dc.date.available2010-09-06T07:34:54Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Hepatology, 2006, v. 45 n. 4, p. 553-559en_HK
dc.identifier.issn0168-8278en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77712-
dc.description.abstractBackground/Aims: This study aimed to measure the intrahepatic total hepatitis B virus (HBV) DNA and covalently closed circular DNA (cccDNA) levels in tumor and non-tumor tissues in hepatocellular carcinoma (HCC) patients. Methods: Intrahepatic total HBV DNA and cccDNA were measured in 25 HCC patients (21 hepatitis B surface antigen [HBsAg]-positive and 4 HBsAg-negative) by the Invader® assay. Results: A low level of intrahepatic HBV DNA was detectable in all HBsAg-negative patients. For HBsAg-positive patients, the intrahepatic total HBV DNA levels in the tumor and non-tumor tissues were comparable (P = 0.903). However, the tumor tissues had significantly higher levels of cccDNA (0.35 vs. 0.16 copies/cell, P = 0.030) and higher proportion of intrahepatic HBV DNA in the form of cccDNA (100% vs. 84%, P = 0.004) than the non-tumor tissues. Seventeen out of 21 (81%) tumor tissues had intrahepatic HBV DNA solely in cccDNA form. Analysis of HBV mRNA expression indicated that HBV replication appeared to be lower in the tumor tissues than the non-tumor tissues. Conclusions: Compared to the non-tumor tissues, the levels of HBV replication in the tumor tissues appeared to be lower, and cccDNA was the predominant form of HBV DNA in the tumor tissues. © 2006 European Association for the Study of the Liver.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhepen_HK
dc.relation.ispartofJournal of Hepatologyen_HK
dc.rightsJournal of Hepatology. Copyright © Elsevier BV.en_HK
dc.subjectHepatitis B virusen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectLiveren_HK
dc.subjectTumoren_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshCarcinoma, Hepatocellular - virologyen_HK
dc.subject.meshDNA, Circular - analysis - isolation & purificationen_HK
dc.subject.meshDNA, Viral - analysis - isolation & purificationen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Viralen_HK
dc.subject.meshHepatitis B Surface Antigens - metabolismen_HK
dc.subject.meshHepatitis B virus - geneticsen_HK
dc.subject.meshHepatitis B, Chronic - complications - virologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver - virologyen_HK
dc.subject.meshLiver Neoplasms - virologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshRNA, Messenger - analysis - isolation & purificationen_HK
dc.subject.meshRNA, Viral - analysis - isolation & purificationen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleQuantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-8278&volume=45&spage=553&epage=559&date=2006&atitle=Quantification+of+hepatitis+B+virus+covalently+closed+circular+DNA+in+patients+with+hepatocellular+carcinomaen_HK
dc.identifier.emailWong, DKH: danywong@hku.hken_HK
dc.identifier.emailYuen, MF: mfyuen@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailFung, J: jfung@sicklehut.comen_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityFung, J=rp00518en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jhep.2006.05.014en_HK
dc.identifier.pmid16904225-
dc.identifier.scopuseid_2-s2.0-33748124433en_HK
dc.identifier.hkuros126117en_HK
dc.identifier.hkuros119627-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748124433&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue4en_HK
dc.identifier.spage553en_HK
dc.identifier.epage559en_HK
dc.identifier.isiWOS:000241276100020-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWong, DKH=7401535819en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridPoon, RTP=7103097223en_HK
dc.identifier.scopusauthoridYuen, JCH=7102620480en_HK
dc.identifier.scopusauthoridFung, J=23091109300en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK

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