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Article: Real-time quantification of hepatitis B virus core-promoter and pre-core mutants during hepatitis E antigen seroconversion

TitleReal-time quantification of hepatitis B virus core-promoter and pre-core mutants during hepatitis E antigen seroconversion
Authors
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
Journal Of Hepatology, 2004, v. 40 n. 6, p. 1008-1017 How to Cite?
AbstractBackground/Aims Detection of hepatitis B virus (HBV) core-promoter A 1762T-G1764A and pre-core G1896A mutants has relied on qualitative assays. We tested the hypothesis that the quantity of A1762T-G1764A and G1896A mutants might have clinical impact, by quantifying these mutants before and after HBe antigen (HBeAg) seroconversion in 58 patients. Methods A real-time quantitative- polymerase chain reaction (Q-PCR) was developed, using minor groove binder (MGB)-conjugated TaqMan probes to impart reaction specificity for wildtype/mutant HBV populations. Results Significant quantities (>20%) of core-promoter A1762T-G1764A mutant existed in 65% of patients before and after HBeAg seroconversion, and were significantly changed (>20% increase/decrease) in 13% of patients after seroconversion. Quantity of A1762T-G1764A mutants was positively correlated with alanine aminotransferase (ALT) (P<0.001) and HBV DNA (P<0.001) levels, both before and after HBeAg seroconversion. Significant quantities of pre-core G1896A mutant existed in about 90% of patients before and after HBeAg seroconversion, and were changed in 16% of patients after seroconversion. Quantity of G1896A mutant was negatively correlated with ALT (P=0.044) and HBV DNA (P=0.007) levels. Conclusions The A1762T-G 1764A and G1896A mutants existed in a high proportion of patients before and were unaffected after HbeAg seroconversion. The quantities of A1762T-G1764A mutant were positively and G 1896A mutant negatively correlated with liver inflammation and viral replication. © 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/77442
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPang, Aen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorYuan, HJen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorKwong, YLen_HK
dc.date.accessioned2010-09-06T07:31:56Z-
dc.date.available2010-09-06T07:31:56Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Hepatology, 2004, v. 40 n. 6, p. 1008-1017en_HK
dc.identifier.issn0168-8278en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77442-
dc.description.abstractBackground/Aims Detection of hepatitis B virus (HBV) core-promoter A 1762T-G1764A and pre-core G1896A mutants has relied on qualitative assays. We tested the hypothesis that the quantity of A1762T-G1764A and G1896A mutants might have clinical impact, by quantifying these mutants before and after HBe antigen (HBeAg) seroconversion in 58 patients. Methods A real-time quantitative- polymerase chain reaction (Q-PCR) was developed, using minor groove binder (MGB)-conjugated TaqMan probes to impart reaction specificity for wildtype/mutant HBV populations. Results Significant quantities (>20%) of core-promoter A1762T-G1764A mutant existed in 65% of patients before and after HBeAg seroconversion, and were significantly changed (>20% increase/decrease) in 13% of patients after seroconversion. Quantity of A1762T-G1764A mutants was positively correlated with alanine aminotransferase (ALT) (P<0.001) and HBV DNA (P<0.001) levels, both before and after HBeAg seroconversion. Significant quantities of pre-core G1896A mutant existed in about 90% of patients before and after HBeAg seroconversion, and were changed in 16% of patients after seroconversion. Quantity of G1896A mutant was negatively correlated with ALT (P=0.044) and HBV DNA (P=0.007) levels. Conclusions The A1762T-G 1764A and G1896A mutants existed in a high proportion of patients before and were unaffected after HbeAg seroconversion. The quantities of A1762T-G1764A mutant were positively and G 1896A mutant negatively correlated with liver inflammation and viral replication. © 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhepen_HK
dc.relation.ispartofJournal of Hepatologyen_HK
dc.rightsJournal of Hepatology. Copyright © Elsevier BV.en_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDNA, Viral - genetics - isolation & purificationen_HK
dc.subject.meshHepatitis B Surface Antigensen_HK
dc.subject.meshHepatitis B virus - geneticsen_HK
dc.subject.meshHepatitis E - immunologyen_HK
dc.subject.meshHepatitis E virus - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMutationen_HK
dc.subject.meshOligodeoxyribonucleotidesen_HK
dc.subject.meshPlasmidsen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshViral Core Proteins - geneticsen_HK
dc.titleReal-time quantification of hepatitis B virus core-promoter and pre-core mutants during hepatitis E antigen seroconversionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-8278&volume=40&spage=1008&epage=1017&date=2004&atitle=Real-time+quantification+of+hepatitis+B+virus+core-promoter+and+pre-core+mutants+during+hepatitis+E+antigen+seroconversionen_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0168-8278(04)00098-4en_HK
dc.identifier.pmid15158343-
dc.identifier.scopuseid_2-s2.0-2442697465en_HK
dc.identifier.hkuros87932en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442697465&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1008en_HK
dc.identifier.epage1017en_HK
dc.identifier.isiWOS:000221979400019-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridPang, A=7007044165en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridYuan, HJ=7402446707en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK

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