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Article: Graft-versus-host disease after liver transplantation: documentation by fluorescent in situ hybridisation and human leucocyte antigen typing

TitleGraft-versus-host disease after liver transplantation: documentation by fluorescent in situ hybridisation and human leucocyte antigen typing
Authors
KeywordsDrug reaction
FISH
HLA
Liver transplantation
Sepsis
Issue Date2000
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CTR
Citation
Clinical Transplantation, 2000, v. 14 n. 2, p. 174-177 How to Cite?
AbstractGraft-versus-host disease (GVHD) after liver transplantation is uncommon and the outcome is often fatal. A firm diagnosis of GVHD is difficult because the clinical triad of skin rash, marrow failure and diarrhoea can be indistinguishable from drug reaction or viral infection, and the presence of donor lymphocyte chimerism is not specific. We describe a case of severe GVHD in a female patient after liver transplantation from a male cadaveric donor. Skin biopsy showed characteristic changes of GVHD. Using Y-chromosome-specific fluorescent in situ hybridisation (FISH), male lymphocytes were demonstrated in 10% of marrow cells and in 90% of lymphocytes infiltrating the dermal-epidermal junction. Donor human leucocyte antigens (HLAs) were detected in the peripheral blood, buccal mucosa and skin by polymerase chain reaction. The GVHD subsided with steroid and anti-thymocyte globulin, but recurred on tailing off of treatment. Despite maximum supportive therapy, including random donor leucocyte infusion, and marrow infusion from a HLA-identical sibling, the patient succumbed to sepsis. Our results showed the utility of combining morphological features with molecular techniques using FISH and HLA typing in confirming a diagnosis of GVHD.
Persistent Identifierhttp://hdl.handle.net/10722/77393
ISSN
2015 Impact Factor: 1.844
2015 SCImago Journal Rankings: 0.821
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAu, WYen_HK
dc.contributor.authorMa, SKen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorHawkins, BRen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorLiu, CLen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLo, CMen_HK
dc.date.accessioned2010-09-06T07:31:25Z-
dc.date.available2010-09-06T07:31:25Z-
dc.date.issued2000en_HK
dc.identifier.citationClinical Transplantation, 2000, v. 14 n. 2, p. 174-177en_HK
dc.identifier.issn0902-0063en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77393-
dc.description.abstractGraft-versus-host disease (GVHD) after liver transplantation is uncommon and the outcome is often fatal. A firm diagnosis of GVHD is difficult because the clinical triad of skin rash, marrow failure and diarrhoea can be indistinguishable from drug reaction or viral infection, and the presence of donor lymphocyte chimerism is not specific. We describe a case of severe GVHD in a female patient after liver transplantation from a male cadaveric donor. Skin biopsy showed characteristic changes of GVHD. Using Y-chromosome-specific fluorescent in situ hybridisation (FISH), male lymphocytes were demonstrated in 10% of marrow cells and in 90% of lymphocytes infiltrating the dermal-epidermal junction. Donor human leucocyte antigens (HLAs) were detected in the peripheral blood, buccal mucosa and skin by polymerase chain reaction. The GVHD subsided with steroid and anti-thymocyte globulin, but recurred on tailing off of treatment. Despite maximum supportive therapy, including random donor leucocyte infusion, and marrow infusion from a HLA-identical sibling, the patient succumbed to sepsis. Our results showed the utility of combining morphological features with molecular techniques using FISH and HLA typing in confirming a diagnosis of GVHD.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CTRen_HK
dc.relation.ispartofClinical Transplantationen_HK
dc.subjectDrug reactionen_HK
dc.subjectFISHen_HK
dc.subjectHLAen_HK
dc.subjectLiver transplantationen_HK
dc.subjectSepsisen_HK
dc.subject.meshAdulten_HK
dc.subject.meshBone Marrow Cells - pathologyen_HK
dc.subject.meshCadaveren_HK
dc.subject.meshFatal Outcomeen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGraft vs Host Disease - diagnosis - etiology - pathologyen_HK
dc.subject.meshHLA Antigens - analysisen_HK
dc.subject.meshHistocompatibility Testingen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshLiver Transplantation - immunologyen_HK
dc.subject.meshLymphocytes - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshSkin - pathologyen_HK
dc.subject.meshY Chromosome - geneticsen_HK
dc.titleGraft-versus-host disease after liver transplantation: documentation by fluorescent in situ hybridisation and human leucocyte antigen typingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0902-0063&volume=14&spage=174&epage=177&date=2000&atitle=Graft-versus-host+disease+after+liver+transplantation:+documentation+by+fluorescent+in+situ+hybridisation+and+human+leucocyte+antigen+typingen_HK
dc.identifier.emailMa, SK: sma@pathology.hku.hken_HK
dc.identifier.emailKwong, YL: ylkwong@hku.hken_HK
dc.identifier.emailNg, IOL: iolng@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.authorityMa, SK=rp00506en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1399-0012.2000.140213.xen_HK
dc.identifier.pmid10770425-
dc.identifier.scopuseid_2-s2.0-0034098743en_HK
dc.identifier.hkuros49602en_HK
dc.identifier.hkuros60015-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034098743&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue2en_HK
dc.identifier.spage174en_HK
dc.identifier.epage177en_HK
dc.identifier.isiWOS:000086207900013-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridAu, WY=7202383089en_HK
dc.identifier.scopusauthoridMa, SK=16444895800en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridHawkins, BR=35944486200en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridLiu, CL=7409789712en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridLo, CM=7401771672en_HK

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