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Article: Electrophysiology and glucose transport of human peritoneal mesothelial cells: Implications for peritoneal dialysis

TitleElectrophysiology and glucose transport of human peritoneal mesothelial cells: Implications for peritoneal dialysis
Authors
KeywordsElectrophysiology
Glucose transport
Human peritoneal mesothelial cells
Polyester filter
Issue Date2001
PublisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com
Citation
Peritoneal Dialysis International, 2001, v. 21 n. 2, p. 115-121 How to Cite?
Abstract◆ Objective: To elucidate ionic and glucose transport across human peritoneal mesothelium, we utilized an Ussing chamber setup and studied the electrophysiological characteristics and tissue permeabilities of human peritoneal mesothelial cells (HPMC) to L- and D-glucose. ◆ Methods: Human mesothelial cells were grown on polyester filters (snapwell; Costar, Cambridge, MA, U.S.A.) that, upon confluence, were fitted into Ussing chambers. Transmesothelial resistance and resting potential were determined using electrophysiological techniques. Radiolabeled glucose was added to one side of the chamber and the permeabilities determined by serial sampling in the receptive compartment. ◆ Results: The transmesothelial potential and resistance were 0.54 ± 0.07 mV (apical positive) and 20.4 ± 3.2 Ω·cm2 respectively (mean ± SEM, n = 36). The course of overall transfer of d- and l-glucose was examined using l-glucose as a positive diffusion-plus-leak marker. The permeabilities of HPMC to D-glucose were 3.00 ± 0.26 cm/sec (apical-to-basolateral) and 3.25 ± 0.27 cm/sec (basolateral-to-apical) [n = 6 experiments, p = not significant (NS)], which were not different from those of L-glucose: 3.00 ± 0.30 cm/sec (apical-to-basolateral) and 2.71 ± 0.24 (basolateral-to-apical) (n = 6 experiments, p = NS). ◆ Conclusions: The transepithelial resistance of HPMC is low and the ionic gradient, although it exists, is small and inconsequential. Passive paracellular flow accounts for the majority of transmesothelial glucose transport. The existence of a large paracellular shunt precludes the mesothelial membrane as a clinically relevant osmotic barrier.
Persistent Identifierhttp://hdl.handle.net/10722/76904
ISSN
2015 Impact Factor: 1.298
2015 SCImago Journal Rankings: 0.683
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, FKen_HK
dc.contributor.authorTo, CHen_HK
dc.contributor.authorLeung, JKHen_HK
dc.contributor.authorChan, TMen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2010-09-06T07:26:10Z-
dc.date.available2010-09-06T07:26:10Z-
dc.date.issued2001en_HK
dc.identifier.citationPeritoneal Dialysis International, 2001, v. 21 n. 2, p. 115-121en_HK
dc.identifier.issn0896-8608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76904-
dc.description.abstract◆ Objective: To elucidate ionic and glucose transport across human peritoneal mesothelium, we utilized an Ussing chamber setup and studied the electrophysiological characteristics and tissue permeabilities of human peritoneal mesothelial cells (HPMC) to L- and D-glucose. ◆ Methods: Human mesothelial cells were grown on polyester filters (snapwell; Costar, Cambridge, MA, U.S.A.) that, upon confluence, were fitted into Ussing chambers. Transmesothelial resistance and resting potential were determined using electrophysiological techniques. Radiolabeled glucose was added to one side of the chamber and the permeabilities determined by serial sampling in the receptive compartment. ◆ Results: The transmesothelial potential and resistance were 0.54 ± 0.07 mV (apical positive) and 20.4 ± 3.2 Ω·cm2 respectively (mean ± SEM, n = 36). The course of overall transfer of d- and l-glucose was examined using l-glucose as a positive diffusion-plus-leak marker. The permeabilities of HPMC to D-glucose were 3.00 ± 0.26 cm/sec (apical-to-basolateral) and 3.25 ± 0.27 cm/sec (basolateral-to-apical) [n = 6 experiments, p = not significant (NS)], which were not different from those of L-glucose: 3.00 ± 0.30 cm/sec (apical-to-basolateral) and 2.71 ± 0.24 (basolateral-to-apical) (n = 6 experiments, p = NS). ◆ Conclusions: The transepithelial resistance of HPMC is low and the ionic gradient, although it exists, is small and inconsequential. Passive paracellular flow accounts for the majority of transmesothelial glucose transport. The existence of a large paracellular shunt precludes the mesothelial membrane as a clinically relevant osmotic barrier.en_HK
dc.languageengen_HK
dc.publisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.comen_HK
dc.relation.ispartofPeritoneal Dialysis Internationalen_HK
dc.subjectElectrophysiologyen_HK
dc.subjectGlucose transporten_HK
dc.subjectHuman peritoneal mesothelial cellsen_HK
dc.subjectPolyester filteren_HK
dc.subject.meshBiological Transporten_HK
dc.subject.meshCell Membrane Permeabilityen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshElectric Impedanceen_HK
dc.subject.meshElectrophysiologyen_HK
dc.subject.meshEpithelial Cells - metabolism - physiologyen_HK
dc.subject.meshGlucose - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMembrane Potentialsen_HK
dc.subject.meshPeritoneal Dialysisen_HK
dc.subject.meshPeritoneum - cytology - metabolism - physiologyen_HK
dc.titleElectrophysiology and glucose transport of human peritoneal mesothelial cells: Implications for peritoneal dialysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0896-8608&volume=21&issue=2&spage=115&epage=121&date=2001&atitle=Electrophysiology+and+glucose+transport+of+human+peritoneal+mesothelial+cells:+implications+for+peritoneal+dialysisen_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11330553-
dc.identifier.scopuseid_2-s2.0-0035002610en_HK
dc.identifier.hkuros59915en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035002610&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue2en_HK
dc.identifier.spage115en_HK
dc.identifier.epage121en_HK
dc.identifier.isiWOS:000168245300002-
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridLi, FK=8219093900en_HK
dc.identifier.scopusauthoridTo, CH=36830030200en_HK
dc.identifier.scopusauthoridLeung, JKH=7202180353en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK

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