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Article: The pathogenetic role of immunoglobulin G from patients with systemic lupus erythematosus in the development of lupus pleuritis

TitleThe pathogenetic role of immunoglobulin G from patients with systemic lupus erythematosus in the development of lupus pleuritis
Authors
KeywordsAnti-dsDNA antibodies
Anti-histone antibodies
Anti-nucleohistone antibodies
Lupus pleuritis
Pleural mesothelial cells
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://rheumatology.oxfordjournals.org/
Citation
Rheumatology, 2004, v. 43 n. 3, p. 286-293 How to Cite?
AbstractObjective. To investigate the pathophysiological effect of immunoglobulin G (IgG) from systemic lupus erythematosus (SLE) patients on pleural mesothelial cells and related mechanisms. Methods. Serum IgG from 28 lupus patients and 13 healthy controls was purified by protein-G affinity chromatography. The concentrations of anti-dsDNA-, anti-histone- and/or anti-nucleohistone-containing IgGs were determined by enzyme-linked immunosorbent assay (ELISA). Lupus patients were divided into an active (n=12) and an inactive group (n=16) on the basis of the SLE Disease Activity Index (SLEDAI). The binding of IgG to a human pleural mesothelial cell line (MeT-5A) under different conditions, including pretreatment with DNase and preincubation with exogenous histone, DNA or nucleohistone, was examined using flow cytometry and cellular ELISA. The effect of IgG on MeT-5A cell proliferation was studied using an MTT assay. Gene expression and protein synthesis for interleukin 1β (IL-1β), monocyte chemoattractant protein 1 (MCP-1) and transforming growth factor β1 (TGF-β1) in MeT-5A cells were determined using reverse transcription-polymerase chain reaction and ELISA. Results. The binding of IgG to MeT-5A cells was higher in the active lupus group than the inactive lupus group (P=0.047) and controls (P=0.003). The binding decreased in both lupus groups following pretreatment of MeT-5A cells with DNase. The binding of IgG to MeT-5A cells was greater by 112% in the active lupus group after preincubation with histone (P < 0.001), but not with DNA or nucleohistone. Exposure of MeT-5A cells to IgG from either lupus group induced cell proliferation when compared with IgG from healthy controls (P=0.04). Gene expression and protein synthesis of MCP-1, TGF-β1 and IL-1β in MeT-5A cells were significantly increased after incubation with IgG from patients with active lupus when compared with IgG from the inactive lupus and control groups (P<0.01). The concentration of anti-dsDNA antibodies correlated with the binding of IgG to MeT-5A cells and the synthesis of cytokines by MeT-5A cells. The serum level of anti-histone antibodies in the active lupus group was higher than that in the inactive group (P=0.015) and the serum concentration correlated with cell binding and MCP-1 production. Conclusions. IgG from lupus patients can bind to MeT-5A cells and the binding is modulated by DNA or histone. Binding of anti-dsDNA-containing IgG to MeT-5A cells induces the synthesis of proinflammatory cytokines. Our findings suggest that the binding of anti-dsDNA antibodies, particularly the IgG isotype, to pleural mesothetium plays a direct pathogenetic role in inducing inflammatory injury in the serositis of SLE. © British Society for Rheumatology 2003; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/76539
ISSN
2015 Impact Factor: 4.524
2015 SCImago Journal Rankings: 1.449
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, Hen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorChan, TMen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2010-09-06T07:22:19Z-
dc.date.available2010-09-06T07:22:19Z-
dc.date.issued2004en_HK
dc.identifier.citationRheumatology, 2004, v. 43 n. 3, p. 286-293en_HK
dc.identifier.issn1462-0324en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76539-
dc.description.abstractObjective. To investigate the pathophysiological effect of immunoglobulin G (IgG) from systemic lupus erythematosus (SLE) patients on pleural mesothelial cells and related mechanisms. Methods. Serum IgG from 28 lupus patients and 13 healthy controls was purified by protein-G affinity chromatography. The concentrations of anti-dsDNA-, anti-histone- and/or anti-nucleohistone-containing IgGs were determined by enzyme-linked immunosorbent assay (ELISA). Lupus patients were divided into an active (n=12) and an inactive group (n=16) on the basis of the SLE Disease Activity Index (SLEDAI). The binding of IgG to a human pleural mesothelial cell line (MeT-5A) under different conditions, including pretreatment with DNase and preincubation with exogenous histone, DNA or nucleohistone, was examined using flow cytometry and cellular ELISA. The effect of IgG on MeT-5A cell proliferation was studied using an MTT assay. Gene expression and protein synthesis for interleukin 1β (IL-1β), monocyte chemoattractant protein 1 (MCP-1) and transforming growth factor β1 (TGF-β1) in MeT-5A cells were determined using reverse transcription-polymerase chain reaction and ELISA. Results. The binding of IgG to MeT-5A cells was higher in the active lupus group than the inactive lupus group (P=0.047) and controls (P=0.003). The binding decreased in both lupus groups following pretreatment of MeT-5A cells with DNase. The binding of IgG to MeT-5A cells was greater by 112% in the active lupus group after preincubation with histone (P < 0.001), but not with DNA or nucleohistone. Exposure of MeT-5A cells to IgG from either lupus group induced cell proliferation when compared with IgG from healthy controls (P=0.04). Gene expression and protein synthesis of MCP-1, TGF-β1 and IL-1β in MeT-5A cells were significantly increased after incubation with IgG from patients with active lupus when compared with IgG from the inactive lupus and control groups (P<0.01). The concentration of anti-dsDNA antibodies correlated with the binding of IgG to MeT-5A cells and the synthesis of cytokines by MeT-5A cells. The serum level of anti-histone antibodies in the active lupus group was higher than that in the inactive group (P=0.015) and the serum concentration correlated with cell binding and MCP-1 production. Conclusions. IgG from lupus patients can bind to MeT-5A cells and the binding is modulated by DNA or histone. Binding of anti-dsDNA-containing IgG to MeT-5A cells induces the synthesis of proinflammatory cytokines. Our findings suggest that the binding of anti-dsDNA antibodies, particularly the IgG isotype, to pleural mesothetium plays a direct pathogenetic role in inducing inflammatory injury in the serositis of SLE. © British Society for Rheumatology 2003; all rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://rheumatology.oxfordjournals.org/en_HK
dc.relation.ispartofRheumatologyen_HK
dc.rightsRheumatology. Copyright © S Karger AG.en_HK
dc.subjectAnti-dsDNA antibodiesen_HK
dc.subjectAnti-histone antibodiesen_HK
dc.subjectAnti-nucleohistone antibodiesen_HK
dc.subjectLupus pleuritisen_HK
dc.subjectPleural mesothelial cellsen_HK
dc.subject.meshAcute Diseaseen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAntibodies, Antinuclear - blooden_HK
dc.subject.meshAutoantibodies - blooden_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshCytokines - biosynthesis - geneticsen_HK
dc.subject.meshEpithelial Cells - immunologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshHistones - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoglobulin G - analysis - immunology - pharmacologyen_HK
dc.subject.meshLupus Erythematosus, Systemic - immunologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPleura - immunologyen_HK
dc.subject.meshPleural Diseases - immunologyen_HK
dc.titleThe pathogenetic role of immunoglobulin G from patients with systemic lupus erythematosus in the development of lupus pleuritisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0080-2727&volume=43&issue=3&spage=286&epage=293&date=2004&atitle=The+pathogenetic+role+of+immunoglobulin+G+from+patients+with+systemic+lupus+erythematosus+in+the+development+of+lupus+pleuritisen_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/rheumatology/keh054en_HK
dc.identifier.pmid14623950-
dc.identifier.scopuseid_2-s2.0-1542299627en_HK
dc.identifier.hkuros87425en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542299627&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue3en_HK
dc.identifier.spage286en_HK
dc.identifier.epage293en_HK
dc.identifier.isiWOS:000188990800005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGuo, H=55468645700en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=8108378300en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK

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