Article: CD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation

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TitleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
AuthorsHu, XT1 2
Chen, YW1
Liang, ACT1
Au, WY1
Wong, KY1
Wan, TSK1
Wong, MLY1
Shen, L1
Chan, KK1
Guo, T1
Chu, KM1
Tao, Q3
Chim, CS1
Loong, F1
Choi, WWL1
Lu, L1
So, CC1
Chan, LC1
Kwong, YL1
Liang, RHS1
Srivastava, G1
Issue Date2010
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
CitationBlood, 2010, v. 115 n. 12, p. 2458-2461 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2009-09-238782
AbstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.
ISSN0006-4971
2011 Impact Factor: 9.898
2011 SCImago Journal Rankings: 1.698
DOIhttp://dx.doi.org/10.1182/blood-2009-09-238782
ISI Accession Number IDWOS:000275981900020
Funding AgencyGrant Number
Council of Hong Kong Special Administrative Region, ChinaHKU 7448/04M
Funding Information:

This work was supported by the Research Grants Council of Hong Kong Special Administrative Region, China (grant HKU 7448/04M; G.S. and R.H.S.L.).

ReferencesReferences in Scopus
GrantsRole of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
DC Field
Value
dc.contributor.authorHu, XT
dc.contributor.authorChen, YW
dc.contributor.authorLiang, ACT
dc.contributor.authorAu, WY
dc.contributor.authorWong, KY
dc.contributor.authorWan, TSK
dc.contributor.authorWong, MLY
dc.contributor.authorShen, L
dc.contributor.authorChan, KK
dc.contributor.authorGuo, T
dc.contributor.authorChu, KM
dc.contributor.authorTao, Q
dc.contributor.authorChim, CS
dc.contributor.authorLoong, F
dc.contributor.authorChoi, WWL
dc.contributor.authorLu, L
dc.contributor.authorSo, CC
dc.contributor.authorChan, LC
dc.contributor.authorKwong, YL
dc.contributor.authorLiang, RHS
dc.contributor.authorSrivastava, G
dc.date.accessioned2010-09-06T07:21:54Z
dc.date.available2010-09-06T07:21:54Z
dc.date.issued2010
dc.description.abstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.
dc.description.grantRole of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
dc.description.grantcode9899
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationBlood, 2010, v. 115 n. 12, p. 2458-2461 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2009-09-238782
dc.identifier.doihttp://dx.doi.org/10.1182/blood-2009-09-238782
dc.identifier.epage2461
dc.identifier.hkuros169529
dc.identifier.isiWOS:000275981900020
Funding AgencyGrant Number
Council of Hong Kong Special Administrative Region, ChinaHKU 7448/04M
Funding Information:

This work was supported by the Research Grants Council of Hong Kong Special Administrative Region, China (grant HKU 7448/04M; G.S. and R.H.S.L.).

dc.identifier.issn0006-4971
2011 Impact Factor: 9.898
2011 SCImago Journal Rankings: 1.698
dc.identifier.issue12
dc.identifier.openurl
dc.identifier.pmid20093404
dc.identifier.scopuseid_2-s2.0-77950549271
dc.identifier.spage2458
dc.identifier.urihttp://hdl.handle.net/10722/76500
dc.identifier.volume115
dc.languageeng
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
dc.publisher.placeUnited States
dc.relation.ispartofBlood
dc.relation.referencesReferences in Scopus
dc.subject.meshAntigens, CD44 - genetics - metabolism
dc.subject.meshImmunoglobulin Heavy Chains - genetics
dc.subject.meshLymphoma, Follicular - genetics - pathology
dc.subject.meshLymphoma, Large B-Cell, Diffuse - genetics - pathology
dc.subject.meshStomach Neoplasms - genetics - pathology
dc.titleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Zhejiang University
  3. Prince of Wales Hospital Hong Kong