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Article: CD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation

TitleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
Authors
Issue Date2010
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2010, v. 115 n. 12, p. 2458-2461 How to Cite?
AbstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/76500
ISSN
2014 Impact Factor: 10.452
2014 SCImago Journal Rankings: 5.246
ISI Accession Number ID
Funding AgencyGrant Number
Council of Hong Kong Special Administrative Region, ChinaHKU 7448/04M
Funding Information:

This work was supported by the Research Grants Council of Hong Kong Special Administrative Region, China (grant HKU 7448/04M; G.S. and R.H.S.L.).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorHu, XTen_HK
dc.contributor.authorChen, YWen_HK
dc.contributor.authorLiang, ACTen_HK
dc.contributor.authorAu, WYen_HK
dc.contributor.authorWong, KYen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorWong, MLYen_HK
dc.contributor.authorShen, Len_HK
dc.contributor.authorChan, KKen_HK
dc.contributor.authorGuo, Ten_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorTao, Qen_HK
dc.contributor.authorChim, CSen_HK
dc.contributor.authorLoong, Fen_HK
dc.contributor.authorChoi, WWLen_HK
dc.contributor.authorLu, Len_HK
dc.contributor.authorSo, CCen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T07:21:54Z-
dc.date.available2010-09-06T07:21:54Z-
dc.date.issued2010en_HK
dc.identifier.citationBlood, 2010, v. 115 n. 12, p. 2458-2461en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76500-
dc.description.abstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.subject.meshAntigens, CD44 - genetics - metabolism-
dc.subject.meshImmunoglobulin Heavy Chains - genetics-
dc.subject.meshLymphoma, Follicular - genetics - pathology-
dc.subject.meshLymphoma, Large B-Cell, Diffuse - genetics - pathology-
dc.subject.meshStomach Neoplasms - genetics - pathology-
dc.titleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=115&issue=12&spage=2458&epage=2461&date=2010&atitle=CD44+activation+in+mature+B-cell+malignancies+by+a+novel+recurrent+IGH+translocationen_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.emailChim, CS: jcschim@hku.hken_HK
dc.identifier.emailChoi, WWL: wlchoi@pathology.hku.hken_HK
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_HK
dc.identifier.emailSo, CC: scc@pathology.hku.hken_HK
dc.identifier.emailChan, LC: chanlc@hkucc.hku.hken_HK
dc.identifier.emailKwong, YL: ylkwong@hku.hken_HK
dc.identifier.emailLiang, RHS: rliang@hku.hken_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_HK
dc.identifier.authorityChu, KM=rp00435en_HK
dc.identifier.authorityChim, CS=rp00408en_HK
dc.identifier.authorityChoi, WWL=rp00247en_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.identifier.authoritySo, CC=rp00391en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1182/blood-2009-09-238782en_HK
dc.identifier.pmid20093404en_HK
dc.identifier.scopuseid_2-s2.0-77950549271en_HK
dc.identifier.hkuros169529en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950549271&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2458en_HK
dc.identifier.epage2461en_HK
dc.identifier.eissn1528-0020-
dc.identifier.isiWOS:000275981900020-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectRole of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma-
dc.identifier.scopusauthoridHu, XT=14824949100en_HK
dc.identifier.scopusauthoridChen, YW=7601441119en_HK
dc.identifier.scopusauthoridLiang, ACT=14825292900en_HK
dc.identifier.scopusauthoridAu, WY=7202383089en_HK
dc.identifier.scopusauthoridWong, KY=7404758500en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridWong, MLY=37021112700en_HK
dc.identifier.scopusauthoridShen, L=7401704659en_HK
dc.identifier.scopusauthoridChan, KK=8986914100en_HK
dc.identifier.scopusauthoridGuo, T=16743820700en_HK
dc.identifier.scopusauthoridChu, KM=7402453538en_HK
dc.identifier.scopusauthoridTao, Q=7102578359en_HK
dc.identifier.scopusauthoridChim, CS=7004597253en_HK
dc.identifier.scopusauthoridLoong, F=6602794154en_HK
dc.identifier.scopusauthoridChoi, WWL=25227295700en_HK
dc.identifier.scopusauthoridLu, L=7403963552en_HK
dc.identifier.scopusauthoridSo, CC=7102919978en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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