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Article: CD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
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TitleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
 
AuthorsHu, XT1 2
Chen, YW1
Liang, ACT1
Au, WY1
Wong, KY1
Wan, TSK1
Wong, MLY1
Shen, L1
Chan, KK1
Guo, T1
Chu, KM1
Tao, Q3
Chim, CS1
Loong, F1
Choi, WWL1
Lu, L1
So, CC1
Chan, LC1
Kwong, YL1
Liang, RHS1
Srivastava, G1
 
Issue Date2010
 
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
 
CitationBlood, 2010, v. 115 n. 12, p. 2458-2461 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2009-09-238782
 
AbstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.
 
ISSN0006-4971
2013 Impact Factor: 9.775
 
DOIhttp://dx.doi.org/10.1182/blood-2009-09-238782
 
ISI Accession Number IDWOS:000275981900020
Funding AgencyGrant Number
Council of Hong Kong Special Administrative Region, ChinaHKU 7448/04M
Funding Information:

This work was supported by the Research Grants Council of Hong Kong Special Administrative Region, China (grant HKU 7448/04M; G.S. and R.H.S.L.).

 
ReferencesReferences in Scopus
 
GrantsRole of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
 
DC FieldValue
dc.contributor.authorHu, XT
 
dc.contributor.authorChen, YW
 
dc.contributor.authorLiang, ACT
 
dc.contributor.authorAu, WY
 
dc.contributor.authorWong, KY
 
dc.contributor.authorWan, TSK
 
dc.contributor.authorWong, MLY
 
dc.contributor.authorShen, L
 
dc.contributor.authorChan, KK
 
dc.contributor.authorGuo, T
 
dc.contributor.authorChu, KM
 
dc.contributor.authorTao, Q
 
dc.contributor.authorChim, CS
 
dc.contributor.authorLoong, F
 
dc.contributor.authorChoi, WWL
 
dc.contributor.authorLu, L
 
dc.contributor.authorSo, CC
 
dc.contributor.authorChan, LC
 
dc.contributor.authorKwong, YL
 
dc.contributor.authorLiang, RHS
 
dc.contributor.authorSrivastava, G
 
dc.date.accessioned2010-09-06T07:21:54Z
 
dc.date.available2010-09-06T07:21:54Z
 
dc.date.issued2010
 
dc.description.abstractUsing inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHSμ were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHSμ/CD44 translocations substitute Sμ for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of Iμ-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44ΔEx1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44ΔEx1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44ΔEx1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. © 2010 by The American Society of Hematology.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationBlood, 2010, v. 115 n. 12, p. 2458-2461 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2009-09-238782
 
dc.identifier.doihttp://dx.doi.org/10.1182/blood-2009-09-238782
 
dc.identifier.eissn1528-0020
 
dc.identifier.epage2461
 
dc.identifier.hkuros169529
 
dc.identifier.isiWOS:000275981900020
Funding AgencyGrant Number
Council of Hong Kong Special Administrative Region, ChinaHKU 7448/04M
Funding Information:

This work was supported by the Research Grants Council of Hong Kong Special Administrative Region, China (grant HKU 7448/04M; G.S. and R.H.S.L.).

 
dc.identifier.issn0006-4971
2013 Impact Factor: 9.775
 
dc.identifier.issue12
 
dc.identifier.openurl
 
dc.identifier.pmid20093404
 
dc.identifier.scopuseid_2-s2.0-77950549271
 
dc.identifier.spage2458
 
dc.identifier.urihttp://hdl.handle.net/10722/76500
 
dc.identifier.volume115
 
dc.languageeng
 
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBlood
 
dc.relation.projectRole of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntigens, CD44 - genetics - metabolism
 
dc.subject.meshImmunoglobulin Heavy Chains - genetics
 
dc.subject.meshLymphoma, Follicular - genetics - pathology
 
dc.subject.meshLymphoma, Large B-Cell, Diffuse - genetics - pathology
 
dc.subject.meshStomach Neoplasms - genetics - pathology
 
dc.titleCD44 activation in mature B-cell malignancies by a novel recurrent IGH translocation
 
dc.typeArticle
 
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<description.abstract>Using inverse polymerase chain reaction, we identified CD44, located on chromosome 11p13, as a novel translocation partner of IGH in 9 of 114 cases of gastric, nongastric extranodal, follicular, and nodal diffuse large B-cell lymphoma (DLBCL). Notably, these translocations involving IGHS&#956; were detected in follicular lymphomas and exclusively in germinal center B cell - like (GCB) - DLBCLs. CD44 is not expressed in reactive GC B cells. The IGHS&#956;/CD44 translocations substitute S&#956; for the CD44 promoter and remove exon 1 of CD44, resulting in the overexpression of I&#956;-CD44 hybrid mRNA transcripts activated from derivative 11 that encode a new CD44 variant lacking the leader peptide and with a unique C-terminus (CD44&#916;Ex1). When overexpressed in vitro in the CD44 - GCB-DLBCL cell line BJAB, CD44&#916;Ex1 - green fluorescent protein localized to the cytoplasm and nucleus, whereas CD44s - green fluorescent protein (standard form) localized to the plasma membrane. The ectopic expression of CD44&#916;Ex1 in BJAB cells enhanced their proliferation rate and clonogenic ability, indicating a possible pathogenic role of the translocation. &#169; 2010 by The American Society of Hematology.</description.abstract>
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<subject.mesh>Antigens, CD44 - genetics - metabolism</subject.mesh>
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<subject.mesh>Lymphoma, Follicular - genetics - pathology</subject.mesh>
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Author Affiliations
  1. The University of Hong Kong
  2. Zhejiang University
  3. Prince of Wales Hospital Hong Kong