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Article: Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism

TitleTwo subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism
Authors
KeywordsHepatitis B virus
Single nucleotides polymorphisms
Subgenotypes
Issue Date2005
PublisherBlackwell Publishing Ltd.
Citation
Hepatology Research, 2005, v. 33 n. 3, p. 216-224 How to Cite?
AbstractRecently hepatitis B virus genotype C (HBV/C) has been classified into geographically typical two subtypes (subgenotypes); HBV/C1 in Southeast Asia (Cs) and HBV/C2 in East Asia (Ce). Our aim is to develop a rapid subtyping assay and to examine the virological features of these two subtypes. Based on 171 HBV/C strains retrieved from the database, 17 single nucleotides polymorphisms (SNPs) were found between two subtypes. Taking advantage of five SNPs in non-overlapping polymerase region, a restriction fragment length polymporphism method with three endonucleases was newly developed for distinguishing between HBV/Cs and HBV/Ce. The method was applied to 49 HBV/C carriers from Japan and Hong Kong. The 24 in Hong Kong were classified into HBV/Cs, and the 25 in Japan were HBV/Ce, confirmed by sequencing. Some specific mutations were detected in the encapsidation signal; precore stop mutation (A1896), accompanied by a C-to-T substitution at nt 1858, was found in HBV/Ce strains, and another precore mutation (A1898), accompanied by a C-to-T mutation at nt 1856, was found in HBV/Cs. Especially, two closely linked mutations (A1896 and A1899) in HBV/Ce could stabilize the epsilon loop structure more efficiently and influece viral replication. Hence, these virological differences between the two subtypes might influence clinical features. © 2005 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/76405
ISSN
2015 Impact Factor: 2.208
2015 SCImago Journal Rankings: 0.782
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTanaka, Yen_HK
dc.contributor.authorOrito, Een_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorMukaide, Men_HK
dc.contributor.authorSugauchi, Fen_HK
dc.contributor.authorIto, Ken_HK
dc.contributor.authorOzasa, Aen_HK
dc.contributor.authorSakamoto, Ten_HK
dc.contributor.authorKurbanov, Fen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorMizokami, Men_HK
dc.date.accessioned2010-09-06T07:20:52Z-
dc.date.available2010-09-06T07:20:52Z-
dc.date.issued2005en_HK
dc.identifier.citationHepatology Research, 2005, v. 33 n. 3, p. 216-224en_HK
dc.identifier.issn1386-6346en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76405-
dc.description.abstractRecently hepatitis B virus genotype C (HBV/C) has been classified into geographically typical two subtypes (subgenotypes); HBV/C1 in Southeast Asia (Cs) and HBV/C2 in East Asia (Ce). Our aim is to develop a rapid subtyping assay and to examine the virological features of these two subtypes. Based on 171 HBV/C strains retrieved from the database, 17 single nucleotides polymorphisms (SNPs) were found between two subtypes. Taking advantage of five SNPs in non-overlapping polymerase region, a restriction fragment length polymporphism method with three endonucleases was newly developed for distinguishing between HBV/Cs and HBV/Ce. The method was applied to 49 HBV/C carriers from Japan and Hong Kong. The 24 in Hong Kong were classified into HBV/Cs, and the 25 in Japan were HBV/Ce, confirmed by sequencing. Some specific mutations were detected in the encapsidation signal; precore stop mutation (A1896), accompanied by a C-to-T substitution at nt 1858, was found in HBV/Ce strains, and another precore mutation (A1898), accompanied by a C-to-T mutation at nt 1856, was found in HBV/Cs. Especially, two closely linked mutations (A1896 and A1899) in HBV/Ce could stabilize the epsilon loop structure more efficiently and influece viral replication. Hence, these virological differences between the two subtypes might influence clinical features. © 2005 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofHepatology Researchen_HK
dc.rightsHepatology Research. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectHepatitis B virusen_HK
dc.subjectSingle nucleotides polymorphismsen_HK
dc.subjectSubgenotypesen_HK
dc.titleTwo subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphismen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6346&volume=33&spage=216&epage=224&date=2005&atitle=Two+Subtypes+(Subgenotypes)+of+Hepatitis+B+Virus+Genotype+C:+A+Novel+Subtyping+Assay+Based+on+Restriction+Fragment+Length+Polymorphismen_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.hepres.2005.08.006en_HK
dc.identifier.scopuseid_2-s2.0-29144491553en_HK
dc.identifier.hkuros115274en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29144491553&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue3en_HK
dc.identifier.spage216en_HK
dc.identifier.epage224en_HK
dc.identifier.isiWOS:000234043900006-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridTanaka, Y=7405315865en_HK
dc.identifier.scopusauthoridOrito, E=7006161634en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridMukaide, M=6701669224en_HK
dc.identifier.scopusauthoridSugauchi, F=7004837927en_HK
dc.identifier.scopusauthoridIto, K=9242748800en_HK
dc.identifier.scopusauthoridOzasa, A=9242748900en_HK
dc.identifier.scopusauthoridSakamoto, T=22939150600en_HK
dc.identifier.scopusauthoridKurbanov, F=7003649588en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridMizokami, M=7103318255en_HK

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