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Article: Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism
Title | Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism |
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Authors | |
Keywords | Hepatitis B virus Single nucleotides polymorphisms Subgenotypes |
Issue Date | 2005 |
Publisher | Blackwell Publishing Ltd. |
Citation | Hepatology Research, 2005, v. 33 n. 3, p. 216-224 How to Cite? |
Abstract | Recently hepatitis B virus genotype C (HBV/C) has been classified into geographically typical two subtypes (subgenotypes); HBV/C1 in Southeast Asia (Cs) and HBV/C2 in East Asia (Ce). Our aim is to develop a rapid subtyping assay and to examine the virological features of these two subtypes. Based on 171 HBV/C strains retrieved from the database, 17 single nucleotides polymorphisms (SNPs) were found between two subtypes. Taking advantage of five SNPs in non-overlapping polymerase region, a restriction fragment length polymporphism method with three endonucleases was newly developed for distinguishing between HBV/Cs and HBV/Ce. The method was applied to 49 HBV/C carriers from Japan and Hong Kong. The 24 in Hong Kong were classified into HBV/Cs, and the 25 in Japan were HBV/Ce, confirmed by sequencing. Some specific mutations were detected in the encapsidation signal; precore stop mutation (A1896), accompanied by a C-to-T substitution at nt 1858, was found in HBV/Ce strains, and another precore mutation (A1898), accompanied by a C-to-T mutation at nt 1856, was found in HBV/Cs. Especially, two closely linked mutations (A1896 and A1899) in HBV/Ce could stabilize the epsilon loop structure more efficiently and influece viral replication. Hence, these virological differences between the two subtypes might influence clinical features. © 2005 Elsevier Ireland Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/76405 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.241 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tanaka, Y | en_HK |
dc.contributor.author | Orito, E | en_HK |
dc.contributor.author | Yuen, MF | en_HK |
dc.contributor.author | Mukaide, M | en_HK |
dc.contributor.author | Sugauchi, F | en_HK |
dc.contributor.author | Ito, K | en_HK |
dc.contributor.author | Ozasa, A | en_HK |
dc.contributor.author | Sakamoto, T | en_HK |
dc.contributor.author | Kurbanov, F | en_HK |
dc.contributor.author | Lai, CL | en_HK |
dc.contributor.author | Mizokami, M | en_HK |
dc.date.accessioned | 2010-09-06T07:20:52Z | - |
dc.date.available | 2010-09-06T07:20:52Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Hepatology Research, 2005, v. 33 n. 3, p. 216-224 | en_HK |
dc.identifier.issn | 1386-6346 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/76405 | - |
dc.description.abstract | Recently hepatitis B virus genotype C (HBV/C) has been classified into geographically typical two subtypes (subgenotypes); HBV/C1 in Southeast Asia (Cs) and HBV/C2 in East Asia (Ce). Our aim is to develop a rapid subtyping assay and to examine the virological features of these two subtypes. Based on 171 HBV/C strains retrieved from the database, 17 single nucleotides polymorphisms (SNPs) were found between two subtypes. Taking advantage of five SNPs in non-overlapping polymerase region, a restriction fragment length polymporphism method with three endonucleases was newly developed for distinguishing between HBV/Cs and HBV/Ce. The method was applied to 49 HBV/C carriers from Japan and Hong Kong. The 24 in Hong Kong were classified into HBV/Cs, and the 25 in Japan were HBV/Ce, confirmed by sequencing. Some specific mutations were detected in the encapsidation signal; precore stop mutation (A1896), accompanied by a C-to-T substitution at nt 1858, was found in HBV/Ce strains, and another precore mutation (A1898), accompanied by a C-to-T mutation at nt 1856, was found in HBV/Cs. Especially, two closely linked mutations (A1896 and A1899) in HBV/Ce could stabilize the epsilon loop structure more efficiently and influece viral replication. Hence, these virological differences between the two subtypes might influence clinical features. © 2005 Elsevier Ireland Ltd. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Blackwell Publishing Ltd. | en_HK |
dc.relation.ispartof | Hepatology Research | en_HK |
dc.rights | Hepatology Research. Copyright © Blackwell Publishing Ltd. | en_HK |
dc.subject | Hepatitis B virus | en_HK |
dc.subject | Single nucleotides polymorphisms | en_HK |
dc.subject | Subgenotypes | en_HK |
dc.title | Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6346&volume=33&spage=216&epage=224&date=2005&atitle=Two+Subtypes+(Subgenotypes)+of+Hepatitis+B+Virus+Genotype+C:+A+Novel+Subtyping+Assay+Based+on+Restriction+Fragment+Length+Polymorphism | en_HK |
dc.identifier.email | Yuen, MF:mfyuen@hkucc.hku.hk | en_HK |
dc.identifier.email | Lai, CL:hrmelcl@hku.hk | en_HK |
dc.identifier.authority | Yuen, MF=rp00479 | en_HK |
dc.identifier.authority | Lai, CL=rp00314 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.hepres.2005.08.006 | en_HK |
dc.identifier.scopus | eid_2-s2.0-29144491553 | en_HK |
dc.identifier.hkuros | 115274 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-29144491553&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 33 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 216 | en_HK |
dc.identifier.epage | 224 | en_HK |
dc.identifier.isi | WOS:000234043900006 | - |
dc.publisher.place | Ireland | en_HK |
dc.identifier.scopusauthorid | Tanaka, Y=7405315865 | en_HK |
dc.identifier.scopusauthorid | Orito, E=7006161634 | en_HK |
dc.identifier.scopusauthorid | Yuen, MF=7102031955 | en_HK |
dc.identifier.scopusauthorid | Mukaide, M=6701669224 | en_HK |
dc.identifier.scopusauthorid | Sugauchi, F=7004837927 | en_HK |
dc.identifier.scopusauthorid | Ito, K=9242748800 | en_HK |
dc.identifier.scopusauthorid | Ozasa, A=9242748900 | en_HK |
dc.identifier.scopusauthorid | Sakamoto, T=22939150600 | en_HK |
dc.identifier.scopusauthorid | Kurbanov, F=7003649588 | en_HK |
dc.identifier.scopusauthorid | Lai, CL=7403086396 | en_HK |
dc.identifier.scopusauthorid | Mizokami, M=7103318255 | en_HK |
dc.identifier.issnl | 1386-6346 | - |