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Article: Imbalance between cell proliferation and cellular DNA fragmentation in hepatocellular carcinoma

TitleImbalance between cell proliferation and cellular DNA fragmentation in hepatocellular carcinoma
Authors
Issue Date1999
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/LIV
Citation
Liver, 1999, v. 19 n. 5, p. 444-451 How to Cite?
AbstractAim/Background: Hepatocellular carcinoma (HCC) is known for its rapid growth. This study was undertaken to determine the expression of proliferative markers, apoptosis (DNA fragmentation) and oncogene products known to regulate apoptosis (p53, bcl-2) in HCC. Methods: 150 Chinese patients with HCC were studied (M:F 128:22, age 14-88 years). Immunohistochemistry was employed to detect cell proliferative markers (PCNA, Ki67), and oncogene products known to regulate apoptosis (p53, bcl-2). DNA fragmentation was determined by terminal dUTP nick end labeling (TUNEL). Results: 98% and 95% of HCC bad PCNA (median 2+) and Ki67 (median 2+) detected respectively. TUNEL labeling was detected in only a small number of tumor cells (no labeling in 11%, median 1/1000 cell labeled, range: 0-70/1000 cells). There was no correlation between TUNEL labeling and the clinical parameters (sex, age, cirrhosis, and survival) and the expression of cell proliferative markers. p53 was detected in 53% of the patients (median 1+, range: 0-4+) and bcl-2 was detected in a small proportion of tumor cells in only 13% of the HCCs (range: 0-1+). The expression of p53 and Bcl-2 did not correlate with TUNEL labeling or the natural survival. Conclusions: Cell proliferation in HCC is unmatched by apoptosis, accounting for the rapid growth of this tumor. This lack of apoptosis in HCC is unrelated to the expression of p53 or bcl-2 over-expression.
Persistent Identifierhttp://hdl.handle.net/10722/76239
ISSN
2004 Impact Factor: 2.379
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, PCen_HK
dc.contributor.authorLau, VKTen_HK
dc.contributor.authorFang, JWSen_HK
dc.contributor.authorLai, VCHen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorLau, JYNen_HK
dc.date.accessioned2010-09-06T07:19:06Z-
dc.date.available2010-09-06T07:19:06Z-
dc.date.issued1999en_HK
dc.identifier.citationLiver, 1999, v. 19 n. 5, p. 444-451en_HK
dc.identifier.issn0106-9543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76239-
dc.description.abstractAim/Background: Hepatocellular carcinoma (HCC) is known for its rapid growth. This study was undertaken to determine the expression of proliferative markers, apoptosis (DNA fragmentation) and oncogene products known to regulate apoptosis (p53, bcl-2) in HCC. Methods: 150 Chinese patients with HCC were studied (M:F 128:22, age 14-88 years). Immunohistochemistry was employed to detect cell proliferative markers (PCNA, Ki67), and oncogene products known to regulate apoptosis (p53, bcl-2). DNA fragmentation was determined by terminal dUTP nick end labeling (TUNEL). Results: 98% and 95% of HCC bad PCNA (median 2+) and Ki67 (median 2+) detected respectively. TUNEL labeling was detected in only a small number of tumor cells (no labeling in 11%, median 1/1000 cell labeled, range: 0-70/1000 cells). There was no correlation between TUNEL labeling and the clinical parameters (sex, age, cirrhosis, and survival) and the expression of cell proliferative markers. p53 was detected in 53% of the patients (median 1+, range: 0-4+) and bcl-2 was detected in a small proportion of tumor cells in only 13% of the HCCs (range: 0-1+). The expression of p53 and Bcl-2 did not correlate with TUNEL labeling or the natural survival. Conclusions: Cell proliferation in HCC is unmatched by apoptosis, accounting for the rapid growth of this tumor. This lack of apoptosis in HCC is unrelated to the expression of p53 or bcl-2 over-expression.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/LIVen_HK
dc.relation.ispartofLiveren_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshCarcinoma, Hepatocellular - genetics - metabolism - pathologyen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshDNA Fragmentationen_HK
dc.subject.meshDNA, Neoplasm - analysisen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshIn Situ Nick-End Labelingen_HK
dc.subject.meshKi-67 Antigen - metabolismen_HK
dc.subject.meshLiver Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshProliferating Cell Nuclear Antigen - metabolismen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - metabolismen_HK
dc.subject.meshTumor Suppressor Protein p53 - metabolismen_HK
dc.titleImbalance between cell proliferation and cellular DNA fragmentation in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0106-9543&volume=19&issue=5&spage=444&epage=451&date=1999&atitle=Imbalance+between+cell+proliferation+and+cellular+DNA+fragmentation+in+hepatocellular+carcinomaen_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1478-3231.1999.tb00076.xen_HK
dc.identifier.pmid10533805-
dc.identifier.scopuseid_2-s2.0-0032873597en_HK
dc.identifier.hkuros48383en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032873597&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue5en_HK
dc.identifier.spage444en_HK
dc.identifier.epage451en_HK
dc.identifier.isiWOS:000082966200014-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridWu, PC=7403119323en_HK
dc.identifier.scopusauthoridLau, VKT=36746286100en_HK
dc.identifier.scopusauthoridFang, JWS=7402963750en_HK
dc.identifier.scopusauthoridLai, VCH=7006205374en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridLau, JYN=7402446047en_HK

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