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Article: Establishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line HKESC-4 of Chinese origin

TitleEstablishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line HKESC-4 of Chinese origin
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
Citation
Cancer Genetics And Cytogenetics, 2007, v. 178 n. 1, p. 17-25 How to Cite?
AbstractA new human esophageal cancer cell line, HKESC-4, was established from a nude-mouse xenograft of a moderately differentiated esophageal squamous cell carcinoma (ESCC) developed from a 65-year-old Hong Kong Chinese man. The cellular characteristics (morphological, electron microscopic, and immunohistochemical studies), tumorigenicity in athymic nude mice, cytogenetic features, and DNA ploidy of the cell line were investigated. The cell line was maintained in vitro for 17 months and passaged 80 times. HKESC-4 grew as a monolayer, with a doubling time of 63 hours. The epithelial nature of HKESC-4 included the presence of cytokeratin intermediate filaments, as shown by antibodies (AE1/AF3, CAM5.2, and MAK 6), and the presence of the tonofilaments, as seen under electron microscopy. HKESC-4 was tumorigenic in nude mice and had DNA aneuploidy. The cytogenetic abnormalities of HKESC-4 included -1, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -15, -16, -17, -18, -19, +20, -21, -22, +del(11)(p11), +i(11)(q10), and +21 marker chromosomes. Comparative genomic hybridization analysis demonstrated chromosomal gains at 1p36.13, 3q23∼q28, 5p15.33∼p15.1, 6p25.1∼p22.3, 7p21.3∼p11.2, 7q11.21∼q21.13, 8q23.3∼q23.3, 11p11.2, 11q12.1∼q13.2, 14q21.3∼q32.2, 17p13.3, 18p11.32∼p11.31, and 20p13∼p12.2 and chromosomal losses at 1q12, 2p25.1∼p24.3, 13p13∼p11.2, 21p, 22p13∼p11.2, and Y. The newly established cell line HKESC-4 promises to be a useful tool in future studies of molecular pathogenesis and therapeutics in ESCC. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71935
ISSN
2012 Impact Factor: 1.929
2013 SCImago Journal Rankings: 0.872
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, LCMen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorLee, PYen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorTang, WKen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorLaw, Sen_HK
dc.date.accessioned2010-09-06T06:36:39Z-
dc.date.available2010-09-06T06:36:39Z-
dc.date.issued2007en_HK
dc.identifier.citationCancer Genetics And Cytogenetics, 2007, v. 178 n. 1, p. 17-25en_HK
dc.identifier.issn0165-4608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71935-
dc.description.abstractA new human esophageal cancer cell line, HKESC-4, was established from a nude-mouse xenograft of a moderately differentiated esophageal squamous cell carcinoma (ESCC) developed from a 65-year-old Hong Kong Chinese man. The cellular characteristics (morphological, electron microscopic, and immunohistochemical studies), tumorigenicity in athymic nude mice, cytogenetic features, and DNA ploidy of the cell line were investigated. The cell line was maintained in vitro for 17 months and passaged 80 times. HKESC-4 grew as a monolayer, with a doubling time of 63 hours. The epithelial nature of HKESC-4 included the presence of cytokeratin intermediate filaments, as shown by antibodies (AE1/AF3, CAM5.2, and MAK 6), and the presence of the tonofilaments, as seen under electron microscopy. HKESC-4 was tumorigenic in nude mice and had DNA aneuploidy. The cytogenetic abnormalities of HKESC-4 included -1, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -15, -16, -17, -18, -19, +20, -21, -22, +del(11)(p11), +i(11)(q10), and +21 marker chromosomes. Comparative genomic hybridization analysis demonstrated chromosomal gains at 1p36.13, 3q23∼q28, 5p15.33∼p15.1, 6p25.1∼p22.3, 7p21.3∼p11.2, 7q11.21∼q21.13, 8q23.3∼q23.3, 11p11.2, 11q12.1∼q13.2, 14q21.3∼q32.2, 17p13.3, 18p11.32∼p11.31, and 20p13∼p12.2 and chromosomal losses at 1q12, 2p25.1∼p24.3, 13p13∼p11.2, 21p, 22p13∼p11.2, and Y. The newly established cell line HKESC-4 promises to be a useful tool in future studies of molecular pathogenesis and therapeutics in ESCC. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergeneen_HK
dc.relation.ispartofCancer Genetics and Cytogeneticsen_HK
dc.subject.meshCarcinoma, Squamous Cell - ethnology - metabolism - pathology-
dc.subject.meshCell Line, Tumor-
dc.subject.meshEsophageal Neoplasms - ethnology - metabolism - pathology-
dc.subject.meshNeoplasm Transplantation-
dc.subject.meshNucleic Acid Hybridization-
dc.titleEstablishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line HKESC-4 of Chinese originen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-4608&volume=178&issue=1&spage=17&epage=25&date=2007&atitle=Establishment+and+characterization+of+a+new+xenograft-derived+human+esophageal+squamous+cell+carcinoma+cell+line+HKESC-4+of+Chinese+originen_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cancergencyto.2007.05.026en_HK
dc.identifier.pmid17889704-
dc.identifier.scopuseid_2-s2.0-34548698427en_HK
dc.identifier.hkuros137741en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548698427&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume178en_HK
dc.identifier.issue1en_HK
dc.identifier.spage17en_HK
dc.identifier.epage25en_HK
dc.identifier.isiWOS:000249971100003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, LCM=21740536900en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK
dc.identifier.scopusauthoridLee, PY=8731985700en_HK
dc.identifier.scopusauthoridHu, L=25958137600en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridTang, WK=36678351100en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK

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