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Article: The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer

TitleThe gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer
Authors
Keywordsβ-Cyclodextrin
Biodegradation
Folate
Gene therapy
In vivo test
Polyethylenimine
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2009, v. 30 n. 29, p. 5793-5803 How to Cite?
AbstractThe success of gene therapy relies on a safe and effective gene delivery system. In this communication, we describe the use of folate grafted PEI 600-CyD (H 1) as an effective polyplex-forming plasmid delivery agent with low toxicity. The structures of the polymer and polyplex were characterized, and the in vitro transfection efficiency, cytotoxicity, and in vivo transfection of H 1 were examined. We found that folate molecules were successfully grafted to PEI 600-CyD. At N/P ratios between 5 and 30, the resulting H 1/DNA polyplexes had diameters less than 120 nm and zeta potentials less than 10 mV. In various tumor cell lines examined (U138, U87, B16, and Lovo), the in vitro transfection efficiency of H 1 was more than 50%, which could be improved by the presence of fetal bovine serum or albumin. The cytotoxicity of H 1 was significantly less than high molecular weight PEI-25 kDa. Importantly, in vivo optical imaging showed that the efficiency of H 1-mediated transfection (50 μg luciferase plasmid (pLuc), N/P ratio = 20/1) was comparable to that of adenovirus-mediated luciferase transduction (1 × 10 9 pfu) in melanoma-bearing mice, and it did not induce any toxicity in the tumor tissue. These results clearly show that H 1 is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA and its application warrants further investigation. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/69106
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
Funding AgencyGrant Number
Focus Budget Scheme project at the Chinese University of Hong Kong
National Nature Science Foundation of China30571068
Funding Information:

We would like to thank Wing-Fu Lai and Ka-Chun William Cheung for critical reading of the manuscript, King-Sun Siu for support in chemical synthesis, Fang Yu and Gao Yi for support in vitro experiments, Zan Shen and Long-Fei Huo for support in animal experiments. This work was supported by the Focus Budget Scheme project at the Chinese University of Hong Kong (Project PI: Hsiangfu Kung) and the National Nature Science Foundation of China (30571068).

References

 

DC FieldValueLanguage
dc.contributor.authorYao, Hen_HK
dc.contributor.authorNg, SSen_HK
dc.contributor.authorTucker, WOen_HK
dc.contributor.authorTsang, YKTen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWang, Xmen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorTang, GPen_HK
dc.contributor.authorLin, MCen_HK
dc.date.accessioned2010-09-06T06:10:37Z-
dc.date.available2010-09-06T06:10:37Z-
dc.date.issued2009en_HK
dc.identifier.citationBiomaterials, 2009, v. 30 n. 29, p. 5793-5803en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69106-
dc.description.abstractThe success of gene therapy relies on a safe and effective gene delivery system. In this communication, we describe the use of folate grafted PEI 600-CyD (H 1) as an effective polyplex-forming plasmid delivery agent with low toxicity. The structures of the polymer and polyplex were characterized, and the in vitro transfection efficiency, cytotoxicity, and in vivo transfection of H 1 were examined. We found that folate molecules were successfully grafted to PEI 600-CyD. At N/P ratios between 5 and 30, the resulting H 1/DNA polyplexes had diameters less than 120 nm and zeta potentials less than 10 mV. In various tumor cell lines examined (U138, U87, B16, and Lovo), the in vitro transfection efficiency of H 1 was more than 50%, which could be improved by the presence of fetal bovine serum or albumin. The cytotoxicity of H 1 was significantly less than high molecular weight PEI-25 kDa. Importantly, in vivo optical imaging showed that the efficiency of H 1-mediated transfection (50 μg luciferase plasmid (pLuc), N/P ratio = 20/1) was comparable to that of adenovirus-mediated luciferase transduction (1 × 10 9 pfu) in melanoma-bearing mice, and it did not induce any toxicity in the tumor tissue. These results clearly show that H 1 is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA and its application warrants further investigation. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.subjectβ-Cyclodextrinen_HK
dc.subjectBiodegradationen_HK
dc.subjectFolateen_HK
dc.subjectGene therapyen_HK
dc.subjectIn vivo testen_HK
dc.subjectPolyethylenimineen_HK
dc.subject.meshCyclodextrins - administration and dosage - chemistry - pharmacokinetics-
dc.subject.meshDNA - administration and dosage - chemistry - pharmacokinetics-
dc.subject.meshDrug Carriers - chemistry-
dc.subject.meshImines - chemistry-
dc.subject.meshMelanoma - drug therapy - genetics - metabolism-
dc.titleThe gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymeren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0142-9612&volume=30&issue=29&spage=5793&epage=5803&date=2009&atitle=The+gene+transfection+efficiency+of+a+folate-PEI600-cyclodextrin+nanopolymeren_HK
dc.identifier.emailNg, SS: ssmng@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.emailLin, MC: mcllin@hkucc.hku.hken_HK
dc.identifier.authorityNg, SS=rp00767en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2009.06.051en_HK
dc.identifier.pmid19615741-
dc.identifier.scopuseid_2-s2.0-68549136489en_HK
dc.identifier.hkuros168606en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68549136489&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue29en_HK
dc.identifier.spage5793en_HK
dc.identifier.epage5803en_HK
dc.identifier.isiWOS:000270115200060-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYao, H=13104506400en_HK
dc.identifier.scopusauthoridNg, SS=7403358718en_HK
dc.identifier.scopusauthoridTucker, WO=40262670600en_HK
dc.identifier.scopusauthoridTsang, YKT=34977619600en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWang, Xm=7501856296en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridTang, GP=7401634016en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.citeulike5372542-

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