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Article: Correlation Between the Single-Site CpG Methylation and Expression Silencing of the XAF1 Gene in Human Gastric and Colon Cancers
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TitleCorrelation Between the Single-Site CpG Methylation and Expression Silencing of the XAF1 Gene in Human Gastric and Colon Cancers
 
AuthorsZou, B2 3
Chim, CS1
Zeng, H3
Leung, SY1
Yang, Y1
Tu, SP1
Lin, MCM1
Wang, J1
He, H1
Jiang, SH2
Sun, YW2 1
Yu, LF2 1
Yuen, ST1
Kung, HF1
Wong, BCY1
 
Issue Date2006
 
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
 
CitationGastroenterology, 2006, v. 131 n. 6, p. 1835-1843 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.gastro.2006.09.050
 
AbstractBackground & Aims: X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) antagonizes the anti-caspase activity of XIAP. XAF1 messenger RNA is present in normal tissues but undetectable in various cancers and thus poses a potential tumor suppressor gene. The aim of this study was to examine the novel pattern of methylation of XAF1 in gastric and colon cancers and locate the important CpG sites for transcriptional regulation and tumor progression. Methods: XAF1 expression was detected by reverse-transcription polymerase chain reaction (PCR) and Western blot analysis. Four different fragments around the transcription start site of XAF1 were cloned and examined putative promoter activities by luciferase reporter assay. Each CpG site in fragment F291 was mutated by site-directed mutagenesis technique, and the change of promoter activity of this fragment was detected by luciferase reporter assay. Methylation status of XAF1 was determined by methylation-specific PCR (MSP) and bisulfite DNA sequencing PCR analysis. Results: Down-regulation of XAF1 in association with hypermethylation was detected in 3 of 4 human gastric cancer cell lines and 6 of 8 colon cancer cell lines. Of the 4 promoter fragments, F291 showed the highest promoter activity, which could be down-regulated obviously by the mutation of particular CpG sites. Moreover, aberrant hypermethylation of these important CpG sites was strongly associated with the development of gastric and colon cancers. Conclusions: A cluster of methylated CpG sites instead of CpG islands located in the promoter area resulted in gene silencing of XAF1, and CpGs at -2nd, -1st, and +3rd positions are functionally more important in its transcriptional regulation. © 2006 AGA Institute.
 
ISSN0016-5085
2013 Impact Factor: 13.926
 
DOIhttp://dx.doi.org/10.1053/j.gastro.2006.09.050
 
ISI Accession Number IDWOS:000243031100027
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZou, B
 
dc.contributor.authorChim, CS
 
dc.contributor.authorZeng, H
 
dc.contributor.authorLeung, SY
 
dc.contributor.authorYang, Y
 
dc.contributor.authorTu, SP
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorWang, J
 
dc.contributor.authorHe, H
 
dc.contributor.authorJiang, SH
 
dc.contributor.authorSun, YW
 
dc.contributor.authorYu, LF
 
dc.contributor.authorYuen, ST
 
dc.contributor.authorKung, HF
 
dc.contributor.authorWong, BCY
 
dc.date.accessioned2010-09-06T06:10:35Z
 
dc.date.available2010-09-06T06:10:35Z
 
dc.date.issued2006
 
dc.description.abstractBackground & Aims: X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) antagonizes the anti-caspase activity of XIAP. XAF1 messenger RNA is present in normal tissues but undetectable in various cancers and thus poses a potential tumor suppressor gene. The aim of this study was to examine the novel pattern of methylation of XAF1 in gastric and colon cancers and locate the important CpG sites for transcriptional regulation and tumor progression. Methods: XAF1 expression was detected by reverse-transcription polymerase chain reaction (PCR) and Western blot analysis. Four different fragments around the transcription start site of XAF1 were cloned and examined putative promoter activities by luciferase reporter assay. Each CpG site in fragment F291 was mutated by site-directed mutagenesis technique, and the change of promoter activity of this fragment was detected by luciferase reporter assay. Methylation status of XAF1 was determined by methylation-specific PCR (MSP) and bisulfite DNA sequencing PCR analysis. Results: Down-regulation of XAF1 in association with hypermethylation was detected in 3 of 4 human gastric cancer cell lines and 6 of 8 colon cancer cell lines. Of the 4 promoter fragments, F291 showed the highest promoter activity, which could be down-regulated obviously by the mutation of particular CpG sites. Moreover, aberrant hypermethylation of these important CpG sites was strongly associated with the development of gastric and colon cancers. Conclusions: A cluster of methylated CpG sites instead of CpG islands located in the promoter area resulted in gene silencing of XAF1, and CpGs at -2nd, -1st, and +3rd positions are functionally more important in its transcriptional regulation. © 2006 AGA Institute.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationGastroenterology, 2006, v. 131 n. 6, p. 1835-1843 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.gastro.2006.09.050
 
dc.identifier.doihttp://dx.doi.org/10.1053/j.gastro.2006.09.050
 
dc.identifier.epage1843
 
dc.identifier.hkuros125605
 
dc.identifier.isiWOS:000243031100027
 
dc.identifier.issn0016-5085
2013 Impact Factor: 13.926
 
dc.identifier.issue6
 
dc.identifier.openurl
 
dc.identifier.pmid17087954
 
dc.identifier.scopuseid_2-s2.0-33845645132
 
dc.identifier.spage1835
 
dc.identifier.urihttp://hdl.handle.net/10722/69102
 
dc.identifier.volume131
 
dc.languageeng
 
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
 
dc.publisher.placeUnited States
 
dc.relation.ispartofGastroenterology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshAged, 80 and over
 
dc.subject.meshAntimetabolites, Antineoplastic - pharmacology
 
dc.subject.meshAzacitidine - analogs & derivatives - pharmacology
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshColonic Neoplasms - genetics - metabolism
 
dc.subject.meshCpG Islands
 
dc.subject.meshDNA Methylation
 
dc.subject.meshDNA, Neoplasm - genetics
 
dc.subject.meshDown-Regulation - drug effects
 
dc.subject.meshFemale
 
dc.subject.meshGene Expression Regulation, Neoplastic - drug effects
 
dc.subject.meshGene Silencing
 
dc.subject.meshHumans
 
dc.subject.meshHydroxamic Acids - pharmacology
 
dc.subject.meshIntracellular Signaling Peptides and Proteins
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeoplasm Proteins - genetics - metabolism
 
dc.subject.meshPromoter Regions, Genetic - genetics
 
dc.subject.meshProtein Synthesis Inhibitors - pharmacology
 
dc.subject.meshRNA, Messenger - genetics - metabolism
 
dc.subject.meshStomach Neoplasms - genetics - metabolism
 
dc.titleCorrelation Between the Single-Site CpG Methylation and Expression Silencing of the XAF1 Gene in Human Gastric and Colon Cancers
 
dc.typeArticle
 
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<contributor.author>Chim, CS</contributor.author>
<contributor.author>Zeng, H</contributor.author>
<contributor.author>Leung, SY</contributor.author>
<contributor.author>Yang, Y</contributor.author>
<contributor.author>Tu, SP</contributor.author>
<contributor.author>Lin, MCM</contributor.author>
<contributor.author>Wang, J</contributor.author>
<contributor.author>He, H</contributor.author>
<contributor.author>Jiang, SH</contributor.author>
<contributor.author>Sun, YW</contributor.author>
<contributor.author>Yu, LF</contributor.author>
<contributor.author>Yuen, ST</contributor.author>
<contributor.author>Kung, HF</contributor.author>
<contributor.author>Wong, BCY</contributor.author>
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<description.abstract>Background &amp; Aims: X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) antagonizes the anti-caspase activity of XIAP. XAF1 messenger RNA is present in normal tissues but undetectable in various cancers and thus poses a potential tumor suppressor gene. The aim of this study was to examine the novel pattern of methylation of XAF1 in gastric and colon cancers and locate the important CpG sites for transcriptional regulation and tumor progression. Methods: XAF1 expression was detected by reverse-transcription polymerase chain reaction (PCR) and Western blot analysis. Four different fragments around the transcription start site of XAF1 were cloned and examined putative promoter activities by luciferase reporter assay. Each CpG site in fragment F291 was mutated by site-directed mutagenesis technique, and the change of promoter activity of this fragment was detected by luciferase reporter assay. Methylation status of XAF1 was determined by methylation-specific PCR (MSP) and bisulfite DNA sequencing PCR analysis. Results: Down-regulation of XAF1 in association with hypermethylation was detected in 3 of 4 human gastric cancer cell lines and 6 of 8 colon cancer cell lines. Of the 4 promoter fragments, F291 showed the highest promoter activity, which could be down-regulated obviously by the mutation of particular CpG sites. Moreover, aberrant hypermethylation of these important CpG sites was strongly associated with the development of gastric and colon cancers. Conclusions: A cluster of methylated CpG sites instead of CpG islands located in the promoter area resulted in gene silencing of XAF1, and CpGs at -2nd, -1st, and +3rd positions are functionally more important in its transcriptional regulation. &#169; 2006 AGA Institute.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiaotong University
  3. Guangzhou Medical College