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Article: Hoxb3 deficiency impairs B lymphopoiesis in mouse bone marrow

TitleHoxb3 deficiency impairs B lymphopoiesis in mouse bone marrow
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2007, v. 35 n. 3, p. 465-475 How to Cite?
AbstractObjective: Hox genes are involved in hematopoietic lineage commitment and differentiation. In this study, we investigated the roles of Hoxb3 in hematopoiesis by examining the phenotypes of a Hoxb3 knockout mutant mouse line. Results: Despite previous reports describing the apparently normal phenotype of these mutant mice, we found that by 6 months of age, Hoxb3 -/- mice began to exhibit significantly impaired B lymphopoiesis in the bone marrow (BM). The cellularity was reduced by 30% in mutant BM compared to age- and sex-matched heterozygous and wild-type controls. The population size of B220 +CD43 + progenitor B cells showed a twofold reduction while that of B220 +CD43 -IgM - precursor B cells was decreased fivefold. Sorting-purified Hoxb3 -/- progenitor B cells displayed significantly reduced proliferative response to IL-7 in culture, consistent with our findings of reduced IL-7 receptor expression in Hoxb3 -/- progenitor B cells. However, the peripheral B cell pool in the spleen of Hoxb3 -/- mice was maintained with a similar size as in wild-type littermates. Conclusion: Analysis of T-cell development in the thymus and B1 cell compartment in the peritoneal cavity showed no significant changes. Thus, our findings suggest that the Hoxb3 gene plays an essential role in regulating B lymphopoiesis in the BM of adult mice. © 2007 International Society for Experimental Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/68230
ISSN
2015 Impact Factor: 2.303
2015 SCImago Journal Rankings: 1.341
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKo, KHen_HK
dc.contributor.authorKwan Lam, QLen_HK
dc.contributor.authorZhang, Men_HK
dc.contributor.authorYen Wong, CKen_HK
dc.contributor.authorChun Lo, CKen_HK
dc.contributor.authorKahmeyerGabbe, Men_HK
dc.contributor.authorTsang, WHen_HK
dc.contributor.authorTsang, SLen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorLu, Len_HK
dc.date.accessioned2010-09-06T06:02:36Z-
dc.date.available2010-09-06T06:02:36Z-
dc.date.issued2007en_HK
dc.identifier.citationExperimental Hematology, 2007, v. 35 n. 3, p. 465-475en_HK
dc.identifier.issn0301-472Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68230-
dc.description.abstractObjective: Hox genes are involved in hematopoietic lineage commitment and differentiation. In this study, we investigated the roles of Hoxb3 in hematopoiesis by examining the phenotypes of a Hoxb3 knockout mutant mouse line. Results: Despite previous reports describing the apparently normal phenotype of these mutant mice, we found that by 6 months of age, Hoxb3 -/- mice began to exhibit significantly impaired B lymphopoiesis in the bone marrow (BM). The cellularity was reduced by 30% in mutant BM compared to age- and sex-matched heterozygous and wild-type controls. The population size of B220 +CD43 + progenitor B cells showed a twofold reduction while that of B220 +CD43 -IgM - precursor B cells was decreased fivefold. Sorting-purified Hoxb3 -/- progenitor B cells displayed significantly reduced proliferative response to IL-7 in culture, consistent with our findings of reduced IL-7 receptor expression in Hoxb3 -/- progenitor B cells. However, the peripheral B cell pool in the spleen of Hoxb3 -/- mice was maintained with a similar size as in wild-type littermates. Conclusion: Analysis of T-cell development in the thymus and B1 cell compartment in the peritoneal cavity showed no significant changes. Thus, our findings suggest that the Hoxb3 gene plays an essential role in regulating B lymphopoiesis in the BM of adult mice. © 2007 International Society for Experimental Hematology.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_HK
dc.relation.ispartofExperimental Hematologyen_HK
dc.rightsExperimental Hematology. Copyright © Elsevier Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshB-Lymphocytes - drug effects - immunology - metabolismen_HK
dc.subject.meshBone Marrow Cells - immunology - metabolismen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshHomeodomain Proteins - genetics - physiologyen_HK
dc.subject.meshHomozygoteen_HK
dc.subject.meshInterleukin-7 - pharmacologyen_HK
dc.subject.meshLymphopoiesis - immunologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshT-Lymphocytes - immunology - metabolismen_HK
dc.titleHoxb3 deficiency impairs B lymphopoiesis in mouse bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-472X&volume=35&issue=3&spage=465&epage=75&date=2007&atitle=Hoxb3+deficiency+impairs+B+lymphopoiesis+in+mouse+bone+marrowen_HK
dc.identifier.emailKwan Lam, QL: qlam@pathology.hku.hken_HK
dc.identifier.emailChan, LC: chanlc@hkucc.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hku.hken_HK
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_HK
dc.identifier.authorityKwan Lam, QL=rp00312en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exphem.2006.10.014en_HK
dc.identifier.pmid17309827-
dc.identifier.scopuseid_2-s2.0-33847069599en_HK
dc.identifier.hkuros126175en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847069599&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue3en_HK
dc.identifier.spage465en_HK
dc.identifier.epage475en_HK
dc.identifier.isiWOS:000244605200014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKo, KH=7202688627en_HK
dc.identifier.scopusauthoridKwan Lam, QL=8722491000en_HK
dc.identifier.scopusauthoridZhang, M=8416776200en_HK
dc.identifier.scopusauthoridYen Wong, CK=15849791600en_HK
dc.identifier.scopusauthoridChun Lo, CK=15847736200en_HK
dc.identifier.scopusauthoridKahmeyerGabbe, M=55288481400en_HK
dc.identifier.scopusauthoridTsang, WH=15849274900en_HK
dc.identifier.scopusauthoridTsang, SL=15722984500en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridLu, L=7403963552en_HK

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