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Article: Differential expression, localization and activity of two alternatively spliced isoforms of human APC regulator CDH1

TitleDifferential expression, localization and activity of two alternatively spliced isoforms of human APC regulator CDH1
Authors
KeywordsAnaphase-promoting complex (APC)
CDH1
Cell-cycle control
Issue Date2003
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 2003, v. 374 n. 2, p. 349-358 How to Cite?
AbstractThe timely destruction of key regulators through ubiquitin-mediated proteolysis ensures the orderly progression of the cell cycle. The APC (anaphase-promoting complex) is a major component of this degradation machinery and its activation is required for the execution of critical events. Recent studies have just begun to reveal the complex control of the APC through a regulatory network involving WD40 repeat proteins CDC20 and CDH1. In the present paper, we report on the identification and characterization of human CDH1β, a novel alternatively spliced isoform of CDH1. Both CDH1α and CDH1β can bind to the APC and stimulate the degradation of cyclin B1, but they are differentially expressed in human tissues and cells. CDH1α contains a nuclear localization signal which is absent in CDH1β. Intracellularly, CDH1α appears in the nucleus whereas CDH1β is a predominantly cytoplasmic protein. The forced overexpression of CDH1α in cultured cells correlates with the reduction of nuclear cyclin A, but the steady-state amount of cyclin A does not change noticeably in CDH1β-overexpressed cells. In Xenopus embryos, ectopic overexpression of human CDH1α, but not of CDH1β, induces cell-cycle arrest during the first G1 phase at the midblastula transition. Taken together, our findings document the differential expression, subcellular localization and cell-cycle-regulatory activity of human CDH1 isoforms.
Persistent Identifierhttp://hdl.handle.net/10722/67990
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorNg, RWMen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2010-09-06T06:00:14Z-
dc.date.available2010-09-06T06:00:14Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical Journal, 2003, v. 374 n. 2, p. 349-358en_HK
dc.identifier.issn0264-6021en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67990-
dc.description.abstractThe timely destruction of key regulators through ubiquitin-mediated proteolysis ensures the orderly progression of the cell cycle. The APC (anaphase-promoting complex) is a major component of this degradation machinery and its activation is required for the execution of critical events. Recent studies have just begun to reveal the complex control of the APC through a regulatory network involving WD40 repeat proteins CDC20 and CDH1. In the present paper, we report on the identification and characterization of human CDH1β, a novel alternatively spliced isoform of CDH1. Both CDH1α and CDH1β can bind to the APC and stimulate the degradation of cyclin B1, but they are differentially expressed in human tissues and cells. CDH1α contains a nuclear localization signal which is absent in CDH1β. Intracellularly, CDH1α appears in the nucleus whereas CDH1β is a predominantly cytoplasmic protein. The forced overexpression of CDH1α in cultured cells correlates with the reduction of nuclear cyclin A, but the steady-state amount of cyclin A does not change noticeably in CDH1β-overexpressed cells. In Xenopus embryos, ectopic overexpression of human CDH1α, but not of CDH1β, induces cell-cycle arrest during the first G1 phase at the midblastula transition. Taken together, our findings document the differential expression, subcellular localization and cell-cycle-regulatory activity of human CDH1 isoforms.en_HK
dc.languageengen_HK
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_HK
dc.relation.ispartofBiochemical Journalen_HK
dc.rightsThe final version of record is available at http://www.biochemj.org/bj/374/bj3740349.htm.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAnaphase-promoting complex (APC)en_HK
dc.subjectCDH1en_HK
dc.subjectCell-cycle controlen_HK
dc.subject.meshAlternative Splicing-
dc.subject.meshGene Expression Regulation-
dc.subject.meshLigases - biosynthesis - genetics - metabolism - physiology-
dc.subject.meshProtein Isoforms - biosynthesis - genetics - metabolism - physiology-
dc.subject.meshUbiquitin-Protein Ligase Complexes-
dc.titleDifferential expression, localization and activity of two alternatively spliced isoforms of human APC regulator CDH1en_HK
dc.typeArticleen_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1042/BJ20030600en_HK
dc.identifier.pmid12797865-
dc.identifier.pmcidPMC1223613-
dc.identifier.scopuseid_2-s2.0-0041328591en_HK
dc.identifier.hkuros83710en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0041328591&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume374en_HK
dc.identifier.issue2en_HK
dc.identifier.spage349en_HK
dc.identifier.epage358en_HK
dc.identifier.isiWOS:000185317800008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhou, Y=7405366890en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridNg, RWM=7102153861en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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