Article: Differential expression of insulin-like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia: Combined cDNA suppression subtractive hybridization and microarray study

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TitleDifferential expression of insulin-like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia: Combined cDNA suppression subtractive hybridization and microarray study
AuthorsFeng, HC1
Tsao, SW1
Ngan, HYS1
Xue, WC1
Chiu, PM1
Cheung, ANY1
KeywordscDNA microarray
FTL
Gestational trophoblastic neoplasia
IGFBP1
Suppression subtractive hybridization
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
CitationCancer, 2005, v. 104 n. 11, p. 2409-2416 [How to Cite?]
DOI: http://dx.doi.org/10.1002/cncr.21483
AbstractBACKGROUND. Hydatidiform mole (HM), the most common type of gestational trophoblastic diseases, can be considered as placenta with abnormal chromosome composition with potential of malignant transformation. Few biologic markers can predict subsequent development of persistent gestational trophoblastic neoplasia (GTN) requiring chemotherapy. METHODS. Suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the differential expression pattern of HM that spontaneously regressed and that subsequently developed metastatic GTN. Tissue-specific chips were constructed from the subtracted cDNA libraries, followed by cDNA microarray analysis. Verification by quantitative RNA analysis by real-time polymerase chain reaction (PCR) and immunohistochemical analysis was performed in 23 genotyped complete HM. RESULTS. Sixteen differentially expressed transcripts were identified. Quantitative RNA analysis confirmed down-regulation of ferritin light polypeptide (FTL) (P = 0.037) and insulin-like growth factor binding protein 1 (IGFBP1) (P = 0.037) in HM that subsequently developed GTN when compared with those HM that regressed. Immunohistochemical analysis further confirmed reduced IGFBP1 protein (P = 0.03) expression in HM that developed GTN. CONCLUSIONS. Findings showed that reduced expression of genes related to cell invasion and immunosuppression, especially FTL and IGFBP1, were associated with development of GTN, and this finding may provide a better understanding of the pathogenesis of GTN. The potential application of FTL and IGFBP1 in management of patients with HM should be explored. © 2005 American Cancer Society.
ISSN0008-543X
2011 Impact Factor: 4.771
2011 SCImago Journal Rankings: 0.578
DOIhttp://dx.doi.org/10.1002/cncr.21483
ISI Accession Number IDWOS:000233419200014
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorFeng, HC
dc.contributor.authorTsao, SW
dc.contributor.authorNgan, HYS
dc.contributor.authorXue, WC
dc.contributor.authorChiu, PM
dc.contributor.authorCheung, ANY
dc.date.accessioned2010-09-06T05:59:20Z
dc.date.available2010-09-06T05:59:20Z
dc.date.issued2005
dc.description.abstractBACKGROUND. Hydatidiform mole (HM), the most common type of gestational trophoblastic diseases, can be considered as placenta with abnormal chromosome composition with potential of malignant transformation. Few biologic markers can predict subsequent development of persistent gestational trophoblastic neoplasia (GTN) requiring chemotherapy. METHODS. Suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the differential expression pattern of HM that spontaneously regressed and that subsequently developed metastatic GTN. Tissue-specific chips were constructed from the subtracted cDNA libraries, followed by cDNA microarray analysis. Verification by quantitative RNA analysis by real-time polymerase chain reaction (PCR) and immunohistochemical analysis was performed in 23 genotyped complete HM. RESULTS. Sixteen differentially expressed transcripts were identified. Quantitative RNA analysis confirmed down-regulation of ferritin light polypeptide (FTL) (P = 0.037) and insulin-like growth factor binding protein 1 (IGFBP1) (P = 0.037) in HM that subsequently developed GTN when compared with those HM that regressed. Immunohistochemical analysis further confirmed reduced IGFBP1 protein (P = 0.03) expression in HM that developed GTN. CONCLUSIONS. Findings showed that reduced expression of genes related to cell invasion and immunosuppression, especially FTL and IGFBP1, were associated with development of GTN, and this finding may provide a better understanding of the pathogenesis of GTN. The potential application of FTL and IGFBP1 in management of patients with HM should be explored. © 2005 American Cancer Society.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationCancer, 2005, v. 104 n. 11, p. 2409-2416 [How to Cite?]
DOI: http://dx.doi.org/10.1002/cncr.21483
dc.identifier.doihttp://dx.doi.org/10.1002/cncr.21483
dc.identifier.epage2416
dc.identifier.hkuros109436
dc.identifier.hkuros130540
dc.identifier.isiWOS:000233419200014
dc.identifier.issn0008-543X
2011 Impact Factor: 4.771
2011 SCImago Journal Rankings: 0.578
dc.identifier.issue11
dc.identifier.openurl
dc.identifier.pmid16222695
dc.identifier.scopuseid_2-s2.0-28044446312
dc.identifier.spage2409
dc.identifier.urihttp://hdl.handle.net/10722/67904
dc.identifier.volume104
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
dc.publisher.placeUnited States
dc.relation.ispartofCancer
dc.relation.referencesReferences in Scopus
dc.rightsCancer. Copyright © John Wiley & Sons, Inc.
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshApoferritins
dc.subject.meshDNA, Complementary - genetics
dc.subject.meshFemale
dc.subject.meshFerritins
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization - methods
dc.subject.meshInsulin-Like Growth Factor Binding Protein 1 - genetics
dc.subject.meshMiddle Aged
dc.subject.meshOligonucleotide Array Sequence Analysis - methods
dc.subject.meshPeptides - genetics
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshPregnancy
dc.subject.meshPregnancy Complications, Neoplastic - genetics
dc.subject.meshTrophoblastic Neoplasms - genetics
dc.subject.meshUterine Neoplasms - genetics
dc.subjectcDNA microarray
dc.subjectFTL
dc.subjectGestational trophoblastic neoplasia
dc.subjectIGFBP1
dc.subjectSuppression subtractive hybridization
dc.titleDifferential expression of insulin-like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia: Combined cDNA suppression subtractive hybridization and microarray study
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong