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Article: Establishment and characterization of a human first-trimester extravillous trophoblast cell line (TEV-1)

TitleEstablishment and characterization of a human first-trimester extravillous trophoblast cell line (TEV-1)
Authors
KeywordsCell line
Extravillous trophoblast
First-trimester
IGFBP3
TGFβ
Issue Date2005
PublisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
Citation
Journal Of The Society For Gynecologic Investigation, 2005, v. 12 n. 4, p. e21-e32 How to Cite?
AbstractOBJECTIVE: Research into the biology of human trophoblast invasion has been hampered by a lack of in vitro models. The aim of this study was to establish and characterize a human extravillous trophoblast cell line from the first-trimester placenta. METHODS: Human papillomavirus type 16 (HPV16) E6/E7 genes were stably expressed in primary cultures of first-trimester placenta via a retroviral vector (pLXSN-E6/E7). Several clones were characterized for extravillous trophoblastic properties by reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemistry. The activities of matrix metalloproteinase (MMP)-2 and MMP-9 were examined with gelatin zymography. One clone (TEV-1), which retains all the established criteria for extravillous trophoblasts, was used in microarray analysis with Stanford Human cDNA chip (41, 421 cDNA features) to examine the differential gene expression after treatment of transforming growth factor beta 1 (TGFβ1). The responsive gene to TGFβ1 treatment was confirmed by quantitative real-time PCR. RESULTS: The clonal TEV-1 has been passaged for more than 105 population doublings with no sign of senescence, the activation of telomerase at early passages, and a near-diploid karyotype. TEV-1 cells expressed cytokeratin 7, HLA-G (a histocompatibility antigen, class IB), and CD9 (the cluster of differentiation antigen 9), and secreted active MMP-2 and MMP-9. TGFβ1 treatment altered the gene expression profile of TEV-1 cells with a marked up-regulation of insulin-like growth factor binding protein 3 (IGFBP3), which was confirmed by quantitative real-time PCR. In addition, the TEV-1 was nontumorigenic when injected into nude mice and unable to form colonies in soft agar. CONCLUSION: Phenotypic and biologic characteristics of TEV-1 were shown as the properties of extravillous trophoblasts; thus, the TEV-1 cell line may be used as a cell model in extravillous trophoblast studies. Copyright © by the 2005 Society for Gynecologic Investigation.
Persistent Identifierhttp://hdl.handle.net/10722/67824
ISSN
2008 Impact Factor: 2.333
References

 

DC FieldValueLanguage
dc.contributor.authorHui, CFen_HK
dc.contributor.authorMei, YCen_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorHing, LWen_HK
dc.contributor.authorWui, MLen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:58:35Z-
dc.date.available2010-09-06T05:58:35Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of The Society For Gynecologic Investigation, 2005, v. 12 n. 4, p. e21-e32en_HK
dc.identifier.issn1071-5576en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67824-
dc.description.abstractOBJECTIVE: Research into the biology of human trophoblast invasion has been hampered by a lack of in vitro models. The aim of this study was to establish and characterize a human extravillous trophoblast cell line from the first-trimester placenta. METHODS: Human papillomavirus type 16 (HPV16) E6/E7 genes were stably expressed in primary cultures of first-trimester placenta via a retroviral vector (pLXSN-E6/E7). Several clones were characterized for extravillous trophoblastic properties by reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemistry. The activities of matrix metalloproteinase (MMP)-2 and MMP-9 were examined with gelatin zymography. One clone (TEV-1), which retains all the established criteria for extravillous trophoblasts, was used in microarray analysis with Stanford Human cDNA chip (41, 421 cDNA features) to examine the differential gene expression after treatment of transforming growth factor beta 1 (TGFβ1). The responsive gene to TGFβ1 treatment was confirmed by quantitative real-time PCR. RESULTS: The clonal TEV-1 has been passaged for more than 105 population doublings with no sign of senescence, the activation of telomerase at early passages, and a near-diploid karyotype. TEV-1 cells expressed cytokeratin 7, HLA-G (a histocompatibility antigen, class IB), and CD9 (the cluster of differentiation antigen 9), and secreted active MMP-2 and MMP-9. TGFβ1 treatment altered the gene expression profile of TEV-1 cells with a marked up-regulation of insulin-like growth factor binding protein 3 (IGFBP3), which was confirmed by quantitative real-time PCR. In addition, the TEV-1 was nontumorigenic when injected into nude mice and unable to form colonies in soft agar. CONCLUSION: Phenotypic and biologic characteristics of TEV-1 were shown as the properties of extravillous trophoblasts; thus, the TEV-1 cell line may be used as a cell model in extravillous trophoblast studies. Copyright © by the 2005 Society for Gynecologic Investigation.en_HK
dc.languageengen_HK
dc.publisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.comen_HK
dc.relation.ispartofJournal of the Society for Gynecologic Investigationen_HK
dc.rightsJournal of the Society for Gynecologic Investigation. Copyright © Sage Publications, Inc.en_HK
dc.subjectCell lineen_HK
dc.subjectExtravillous trophoblasten_HK
dc.subjectFirst-trimesteren_HK
dc.subjectIGFBP3en_HK
dc.subjectTGFβen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGenetic Markersen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshOncogene Proteins, Viral - biosynthesisen_HK
dc.subject.meshPapillomavirus E7 Proteinsen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshPlacenta - cytologyen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPregnancy Trimester, Firsten_HK
dc.subject.meshRepressor Proteins - biosynthesisen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTransforming Growth Factor beta - physiologyen_HK
dc.subject.meshTransforming Growth Factor beta1en_HK
dc.subject.meshTrophoblasts - physiologyen_HK
dc.titleEstablishment and characterization of a human first-trimester extravillous trophoblast cell line (TEV-1)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1071-5576&volume=12&issue=4&spage=e21&epage=32&date=2005&atitle=Establishment+and+characterization+of+a+human+first-trimester+extravillous+trophoblast+cell+line+(TEV-1)en_HK
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.authorityDeng, W=rp01640en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authoritySai, WT=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jsgi.2005.02.008en_HK
dc.identifier.pmid15866109-
dc.identifier.scopuseid_2-s2.0-18144428330en_HK
dc.identifier.hkuros101853en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18144428330&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue4en_HK
dc.identifier.spagee21en_HK
dc.identifier.epagee32en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHui, CF=8415265200en_HK
dc.identifier.scopusauthoridMei, YC=8415264500en_HK
dc.identifier.scopusauthoridDeng, W=7202223673en_HK
dc.identifier.scopusauthoridHing, LW=8415264700en_HK
dc.identifier.scopusauthoridWui, ML=8415264800en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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