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Article: Clinical significance of telomerase activation and telomeric restriction fragment (TRF) in cervical cancer

TitleClinical significance of telomerase activation and telomeric restriction fragment (TRF) in cervical cancer
Authors
KeywordsCervical cancers
Telomerase
TRF
Issue Date1999
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca
Citation
European Journal Of Cancer, 1999, v. 35 n. 1, p. 154-160 How to Cite?
AbstractTelomerase activation was examined in 50 cases of cervical cancer, 27 normal cervix and five cervical cancer cell lines using the sensitive polymerase chain reaction (PCR)-based TRAP (telomeric repeat amplification protocol) assay. Telomeric restriction fragment (TRF) length of these specimens was measured by Southern hybridisation. Telomerase activation was common in cervical cancers and was detected in 46/50 cases (92%). Telomerase activity was weak in normal cervix and was detected only in 2/27 cases (7.4%). Telomerase activity was detected in all stages of cervical cancer suggesting that it is an early event in cancer progression. The clinical significance of telomerase activation was analysed in 47 squamous cell carcinoma of the cervix. High telomerase activity was more frequently detected in advanced diseases (100% in stage III and stage IV cervical cancers combined) compared with early diseases (68.6% in stage I and stage II cancers combined). The difference was statistically significant (P < 0.02). Telomerase activity was not statistically correlated with other clinical parameters examined. This is the first report of telomeric length in human cervical cancer. Both shortening and elongation of TRF length in cervical cancers was observed. Advanced cervical cancers tended to have a wider range of variation of TRF length compared with early disease and normal cervix. There was no obvious relationship between TRF length and the clinical parameters examined including clinical staging, differentiation status of tumour, human papilloma virus (HPV) infection, recurrence rate, tumor size and invasion depth. The clinical significance of TRF length appears to be limited in cervical cancers. Our results indicate that telomerase activity is closely associated with tumor cells and may be useful as a marker for detection of tumor cells in cervical biopsies.
Persistent Identifierhttp://hdl.handle.net/10722/67816
ISSN
2015 Impact Factor: 6.163
2015 SCImago Journal Rankings: 3.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, DKen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheng, RYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:58:30Z-
dc.date.available2010-09-06T05:58:30Z-
dc.date.issued1999en_HK
dc.identifier.citationEuropean Journal Of Cancer, 1999, v. 35 n. 1, p. 154-160en_HK
dc.identifier.issn0959-8049en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67816-
dc.description.abstractTelomerase activation was examined in 50 cases of cervical cancer, 27 normal cervix and five cervical cancer cell lines using the sensitive polymerase chain reaction (PCR)-based TRAP (telomeric repeat amplification protocol) assay. Telomeric restriction fragment (TRF) length of these specimens was measured by Southern hybridisation. Telomerase activation was common in cervical cancers and was detected in 46/50 cases (92%). Telomerase activity was weak in normal cervix and was detected only in 2/27 cases (7.4%). Telomerase activity was detected in all stages of cervical cancer suggesting that it is an early event in cancer progression. The clinical significance of telomerase activation was analysed in 47 squamous cell carcinoma of the cervix. High telomerase activity was more frequently detected in advanced diseases (100% in stage III and stage IV cervical cancers combined) compared with early diseases (68.6% in stage I and stage II cancers combined). The difference was statistically significant (P < 0.02). Telomerase activity was not statistically correlated with other clinical parameters examined. This is the first report of telomeric length in human cervical cancer. Both shortening and elongation of TRF length in cervical cancers was observed. Advanced cervical cancers tended to have a wider range of variation of TRF length compared with early disease and normal cervix. There was no obvious relationship between TRF length and the clinical parameters examined including clinical staging, differentiation status of tumour, human papilloma virus (HPV) infection, recurrence rate, tumor size and invasion depth. The clinical significance of TRF length appears to be limited in cervical cancers. Our results indicate that telomerase activity is closely associated with tumor cells and may be useful as a marker for detection of tumor cells in cervical biopsies.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejcaen_HK
dc.relation.ispartofEuropean Journal of Canceren_HK
dc.subjectCervical cancersen_HK
dc.subjectTelomeraseen_HK
dc.subjectTRFen_HK
dc.subject.meshAdenocarcinoma - enzymologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshCarcinoma, Squamous Cell - enzymologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshTelomerase - metabolismen_HK
dc.subject.meshTelomere - enzymology - geneticsen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshUterine Cervical Neoplasms - enzymologyen_HK
dc.titleClinical significance of telomerase activation and telomeric restriction fragment (TRF) in cervical canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0959-8049&volume=35&issue=1&spage=154&epage=160&date=1999&atitle=Clinical+significance+of+telomerase+activation+and+telomeric+restriction+fragment+(TRF)+in+cervical+canceren_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0959-8049(98)00303-7en_HK
dc.identifier.pmid10211104-
dc.identifier.scopuseid_2-s2.0-0032994054en_HK
dc.identifier.hkuros39382en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032994054&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue1en_HK
dc.identifier.spage154en_HK
dc.identifier.epage160en_HK
dc.identifier.isiWOS:000078642500029-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, DK=7405361705en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheng, RYS=7201955319en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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