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Conference Paper: Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum

TitleCytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 145, abstract no. P-7/25 How to Cite?
AbstractLycium barbarum is a commonly used Chinese medicinal herb for nourishment of the eyes, liver and kidney. Studies have shown that extracts from L. barbarum process anti-aging and cytoprotective activities. Its extracts have been demonstrated to exhibit anti-oxidative effects in which oxidative stress is considered to contribute to aging processes. Many studies, therefore, explain the protective effects of L. barbarum as a result of its anti-oxidative activities. In this study, we demonstrated that L. barbarum is not solely an anti-oxidant. We used dithiothreitol (DTT) as reducing agent to trigger reducing stress in primary cultured neurons and in human neuroblastoma SH-SY5Y cell line. We found that extracts of L. barbarum (LBG) exerted cytoprotective effects on both types of cells by lowering the DTT-induced LDH release and activation of caspase-3. DTT is a well-known endoplasmic reticulum (ER)-stress inducer and can cause PKR-like ER kinase (PERK) activation. We found that LBG significantly attenuated the level of DTT-induced phosphor-PERK in primary neuronal cultures. Taken together, our study shows that L. barbarum can exhibit cytoprotective effects against reducing stress on the ER and is therefore not simply an antioxidant. Acknowledgement: This work is partly supported by HKU Strategic Research Theme on Healthy Aging, and HKU Seed Funding for Basic Research (2004–2005) to R.C.C.C.
Persistent Identifierhttp://hdl.handle.net/10722/67814
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763
Patent10722/45673

 

DC FieldValueLanguage
dc.contributor.authorYu, MSen_HK
dc.contributor.authorHo, YSen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-09-06T05:58:29Z-
dc.date.available2010-09-06T05:58:29Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 145, abstract no. P-7/25en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67814-
dc.description.abstractLycium barbarum is a commonly used Chinese medicinal herb for nourishment of the eyes, liver and kidney. Studies have shown that extracts from L. barbarum process anti-aging and cytoprotective activities. Its extracts have been demonstrated to exhibit anti-oxidative effects in which oxidative stress is considered to contribute to aging processes. Many studies, therefore, explain the protective effects of L. barbarum as a result of its anti-oxidative activities. In this study, we demonstrated that L. barbarum is not solely an anti-oxidant. We used dithiothreitol (DTT) as reducing agent to trigger reducing stress in primary cultured neurons and in human neuroblastoma SH-SY5Y cell line. We found that extracts of L. barbarum (LBG) exerted cytoprotective effects on both types of cells by lowering the DTT-induced LDH release and activation of caspase-3. DTT is a well-known endoplasmic reticulum (ER)-stress inducer and can cause PKR-like ER kinase (PERK) activation. We found that LBG significantly attenuated the level of DTT-induced phosphor-PERK in primary neuronal cultures. Taken together, our study shows that L. barbarum can exhibit cytoprotective effects against reducing stress on the ER and is therefore not simply an antioxidant. Acknowledgement: This work is partly supported by HKU Strategic Research Theme on Healthy Aging, and HKU Seed Funding for Basic Research (2004–2005) to R.C.C.C.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignals-
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleCytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulumen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=145&epage=&date=2006&atitle=Cytoprotective+effects+of+Lycium+barbarum+on+cultured+neurons+against+reducing+stress+on+the+endoplasmic+reticulumen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYuen, WH: yuenwh@HKUCC.hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros124881en_HK
dc.identifier.volume15-
dc.identifier.issue3-
dc.identifier.spage145, abstract no. P-7/25-
dc.identifier.epage145, abstract no. P-7/25-
dc.publisher.placeSwitzerland-
dc.description.otherThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December, 2005. In Neurosignals, 2006, v. 15 n. 3, p. 145, abstract no. P-7/25-
dc.relation.patent10722/45673-

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