File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Monochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma

TitleMonochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma
Authors
Issue Date2008
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://mcr.aacrjournals.org/
Citation
Molecular Cancer Research, 2008, v. 6 n. 4, p. 592-603 How to Cite?
Abstract
Loss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity. These findings suggest that THSD1 is a good candidate TSG. Copyright © 2008 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/67774
ISSN
2013 Impact Factor: 4.502
2013 SCImago Journal Rankings: 2.764
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Genome Institute of Singapore
  3. Hong Kong Polytechnic University
  4. Hong Kong University of Science and Technology
  5. UC Irvine
DC FieldValueLanguage
dc.contributor.authorKo, JMYen_HK
dc.contributor.authorPui, LCen_HK
dc.contributor.authorWing, LYen_HK
dc.contributor.authorHo, KCen_HK
dc.contributor.authorKing, CCen_HK
dc.contributor.authorZhuo, YYen_HK
dc.contributor.authorFung, MKen_HK
dc.contributor.authorMiller, LDen_HK
dc.contributor.authorLiu, ETen_HK
dc.contributor.authorLi, CYen_HK
dc.contributor.authorLo, PHYen_HK
dc.contributor.authorStanbridge, EJen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorSai, WTen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2010-09-06T05:58:08Z-
dc.date.available2010-09-06T05:58:08Z-
dc.date.issued2008en_HK
dc.identifier.citationMolecular Cancer Research, 2008, v. 6 n. 4, p. 592-603en_HK
dc.identifier.issn1541-7786en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67774-
dc.description.abstractLoss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity. These findings suggest that THSD1 is a good candidate TSG. Copyright © 2008 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://mcr.aacrjournals.org/en_HK
dc.relation.ispartofMolecular Cancer Researchen_HK
dc.subject.meshAllelesen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshChromosome Segregationen_HK
dc.subject.meshChromosomes, Human, Pair 13 - geneticsen_HK
dc.subject.meshDNA Methylation - drug effectsen_HK
dc.subject.meshDeoxycytidine - pharmacologyen_HK
dc.subject.meshEpithelial Cells - drug effects - pathologyen_HK
dc.subject.meshEsophageal Neoplasms - geneticsen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshGenes, Tumor Suppressoren_HK
dc.subject.meshGenome, Human - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydroxamic Acids - pharmacologyen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshMicroarray Analysisen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshThrombospondins - genetics - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTumor Stem Cell Assayen_HK
dc.titleMonochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1541-7786&volume=6&issue=4&spage=592&epage=603&date=2008&atitle=Monochromosome+transfer+and+microarray+analysis+identify+a+critical+tumor-suppressive+region+mapping+to+chromosome+13q14+and+THSD1+in+esophageal+carcinomaen_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_HK
dc.identifier.emailSai, WT: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailLung, ML: mlilung@hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authoritySai, WT=rp00399en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/1541-7786.MCR-07-0154en_HK
dc.identifier.pmid18403638en_HK
dc.identifier.scopuseid_2-s2.0-42049120045en_HK
dc.identifier.hkuros141625en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42049120045&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue4en_HK
dc.identifier.spage592en_HK
dc.identifier.epage603en_HK
dc.identifier.isiWOS:000254905600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKo, JMY=35725559400en_HK
dc.identifier.scopusauthoridPui, LC=23095469900en_HK
dc.identifier.scopusauthoridWing, LY=15119281300en_HK
dc.identifier.scopusauthoridHo, KC=24068351900en_HK
dc.identifier.scopusauthoridKing, CC=23570603000en_HK
dc.identifier.scopusauthoridZhuo, YY=24069465900en_HK
dc.identifier.scopusauthoridFung, MK=23570461000en_HK
dc.identifier.scopusauthoridMiller, LD=7404985394en_HK
dc.identifier.scopusauthoridLiu, ET=7202240109en_HK
dc.identifier.scopusauthoridLi, CY=36065611200en_HK
dc.identifier.scopusauthoridLo, PHY=36762664000en_HK
dc.identifier.scopusauthoridStanbridge, EJ=7103249410en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridSai, WT=7102813116en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats