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Article: Abnormal expression or mutation of TP53 and HPV in vulvar cancer

TitleAbnormal expression or mutation of TP53 and HPV in vulvar cancer
Authors
KeywordsHPV
p53
VIN
Vulvar cancer
Vulvar dystrophy
Issue Date1999
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca
Citation
European Journal Of Cancer, 1999, v. 35 n. 3, p. 481-484 How to Cite?
AbstractHPV (human papillomavirus) plays an important role in cervical cancer and may also play a role in vulvar cancer. TP53 mutation is common in a variety of cancers but its role in vulvar cancer is not well established. The aim of this study was to assess the prevalence of HPV infection and TP53 mutation as well as their correlation in vulvar cancer. Also, HPV detection and abnormal p53 expression were assessed in relation to age, co-existing vulvar intraepithelial neoplasia and vulvar dystrophy. Forty-eight samples of vulvar cancer were studied. DNA was extracted from formalin-fixed paraffin embedded tissue for polymerase chain reaction/Southern blot study with HPV 16 and 18 and L1 primers. Paraffin sections were immunostained (IHS) for p53 protein using three antibodies, p1801, CM1 and DO7. The p53 mutation was also screened using polymerase chain reaction (PCR) single-stranded conformation polymorphism (SSCP) and confirmed by sequencing. Overall, HPV was detected in 48% (23/48), of which 96% (22/23) were HPV 16 or 18. By IHS, p53 overexpression was detected in 46% of cases whilst TP53 mutations were identified in 21%. In HPV positive and negative tumours, p53 abnormal expression was detected in 39% and 52%, respectively, and TP53 mutation was found in 22% and 20%, respectively. Mutations were mainly found at codons 273 and 204. Age Was not found to be associated with HPV detection. However, the presence of HPV (71%) or absence of abnormal p53 expression (65%) were higher in tumours with VIN3, but were not correlated with dystrophy.
Persistent Identifierhttp://hdl.handle.net/10722/67756
ISSN
2015 Impact Factor: 6.163
2015 SCImago Journal Rankings: 3.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorYip, PSFen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:57:58Z-
dc.date.available2010-09-06T05:57:58Z-
dc.date.issued1999en_HK
dc.identifier.citationEuropean Journal Of Cancer, 1999, v. 35 n. 3, p. 481-484en_HK
dc.identifier.issn0959-8049en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67756-
dc.description.abstractHPV (human papillomavirus) plays an important role in cervical cancer and may also play a role in vulvar cancer. TP53 mutation is common in a variety of cancers but its role in vulvar cancer is not well established. The aim of this study was to assess the prevalence of HPV infection and TP53 mutation as well as their correlation in vulvar cancer. Also, HPV detection and abnormal p53 expression were assessed in relation to age, co-existing vulvar intraepithelial neoplasia and vulvar dystrophy. Forty-eight samples of vulvar cancer were studied. DNA was extracted from formalin-fixed paraffin embedded tissue for polymerase chain reaction/Southern blot study with HPV 16 and 18 and L1 primers. Paraffin sections were immunostained (IHS) for p53 protein using three antibodies, p1801, CM1 and DO7. The p53 mutation was also screened using polymerase chain reaction (PCR) single-stranded conformation polymorphism (SSCP) and confirmed by sequencing. Overall, HPV was detected in 48% (23/48), of which 96% (22/23) were HPV 16 or 18. By IHS, p53 overexpression was detected in 46% of cases whilst TP53 mutations were identified in 21%. In HPV positive and negative tumours, p53 abnormal expression was detected in 39% and 52%, respectively, and TP53 mutation was found in 22% and 20%, respectively. Mutations were mainly found at codons 273 and 204. Age Was not found to be associated with HPV detection. However, the presence of HPV (71%) or absence of abnormal p53 expression (65%) were higher in tumours with VIN3, but were not correlated with dystrophy.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejcaen_HK
dc.relation.ispartofEuropean Journal of Canceren_HK
dc.subjectHPVen_HK
dc.subjectp53en_HK
dc.subjectVINen_HK
dc.subjectVulvar canceren_HK
dc.subjectVulvar dystrophyen_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshCarcinoma, Squamous Cell - genetics - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenes, p53 - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMutation - geneticsen_HK
dc.subject.meshPapillomaviridae - isolation & purificationen_HK
dc.subject.meshPapillomavirus Infections - diagnosis - virologyen_HK
dc.subject.meshTumor Virus Infections - diagnosisen_HK
dc.subject.meshVulvar Neoplasms - genetics - virologyen_HK
dc.titleAbnormal expression or mutation of TP53 and HPV in vulvar canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0959-8049&volume=35&issue=3&spage=481&epage=484&date=1999&atitle=Abnormal+expression+or+mutation+of+TP53+and+HPV+in+vulvar+canceren_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailLiu, SS: stephasl@hku.hken_HK
dc.identifier.emailYip, PSF: sfpyip@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityYip, PSF=rp00596en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0959-8049(98)00407-9en_HK
dc.identifier.pmid10448303-
dc.identifier.scopuseid_2-s2.0-0033105716en_HK
dc.identifier.hkuros41135en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033105716&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue3en_HK
dc.identifier.spage481en_HK
dc.identifier.epage484en_HK
dc.identifier.isiWOS:000079455000032-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridYip, PSF=7102503720en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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