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Article: Correlation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells
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TitleCorrelation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells
 
AuthorsWang, X1
Jin, DY1
Wong, YC1
Cheung, ALM1
Chun, ACS1
Lo, AKF1
Liu, Y1
Tsao, SW1
 
Issue Date2000
 
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
CitationCarcinogenesis, 2000, v. 21 n. 12, p. 2293-2297 [How to Cite?]
 
AbstractNasopharyngeal carcinoma (NPC) occurs with a high incidence in many countries in south-eastern Asia. Chromosomal abnormalities have been commonly found in NPC, but the underlying mechanism is not well understood. We determined mitotic indices, the staining pattern of nuclear DNA and cell cycle profiles of NPC cells in response to treatment with microtubule-disrupting agents, and found that the mitotic checkpoint was defective in two out of five (40%) of the tested NPC cell lines. We also observed that an aberrantly reduced expression of MAD2, one of the key components of mitotic checkpoint, correlated with the loss of checkpoint control. Our findings suggest that a defective mitotic checkpoint characterized by reduced expression of MAD2 contributes to chromosomal instability in NPC.
 
ISSN0143-3334
2012 Impact Factor: 5.635
2012 SCImago Journal Rankings: 2.349
 
ISI Accession Number IDWOS:000166347100021
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, X
 
dc.contributor.authorJin, DY
 
dc.contributor.authorWong, YC
 
dc.contributor.authorCheung, ALM
 
dc.contributor.authorChun, ACS
 
dc.contributor.authorLo, AKF
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorTsao, SW
 
dc.date.accessioned2010-09-06T05:57:45Z
 
dc.date.available2010-09-06T05:57:45Z
 
dc.date.issued2000
 
dc.description.abstractNasopharyngeal carcinoma (NPC) occurs with a high incidence in many countries in south-eastern Asia. Chromosomal abnormalities have been commonly found in NPC, but the underlying mechanism is not well understood. We determined mitotic indices, the staining pattern of nuclear DNA and cell cycle profiles of NPC cells in response to treatment with microtubule-disrupting agents, and found that the mitotic checkpoint was defective in two out of five (40%) of the tested NPC cell lines. We also observed that an aberrantly reduced expression of MAD2, one of the key components of mitotic checkpoint, correlated with the loss of checkpoint control. Our findings suggest that a defective mitotic checkpoint characterized by reduced expression of MAD2 contributes to chromosomal instability in NPC.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationCarcinogenesis, 2000, v. 21 n. 12, p. 2293-2297 [How to Cite?]
 
dc.identifier.epage2297
 
dc.identifier.hkuros56294
 
dc.identifier.isiWOS:000166347100021
 
dc.identifier.issn0143-3334
2012 Impact Factor: 5.635
2012 SCImago Journal Rankings: 2.349
 
dc.identifier.issue12
 
dc.identifier.openurl
 
dc.identifier.pmid11133821
 
dc.identifier.scopuseid_2-s2.0-0034518709
 
dc.identifier.spage2293
 
dc.identifier.urihttp://hdl.handle.net/10722/67732
 
dc.identifier.volume21
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCarcinogenesis
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
 
dc.subject.meshAsia, Southeastern - epidemiology
 
dc.subject.meshDNA, Neoplasm - analysis
 
dc.subject.meshDNA-Binding Proteins - analysis - genetics
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshHeLa Cells
 
dc.subject.meshHumans
 
dc.subject.meshIncidence
 
dc.subject.meshMitosis - drug effects
 
dc.subject.meshMitotic Index
 
dc.subject.meshNasopharyngeal Neoplasms - epidemiology - genetics - pathology
 
dc.subject.meshNocodazole - pharmacology
 
dc.subject.meshSmad2 Protein
 
dc.subject.meshTrans-Activators - analysis - genetics
 
dc.subject.meshTumor Cells, Cultured
 
dc.titleCorrelation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong