Article: Correlation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells

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TitleCorrelation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells
AuthorsWang, X1
Jin, DY1
Wong, YC1
Cheung, ALM1
Chun, ACS1
Lo, AKF1
Liu, Y1
Tsao, SW1
Issue Date2000
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
CitationCarcinogenesis, 2000, v. 21 n. 12, p. 2293-2297 [How to Cite?]
AbstractNasopharyngeal carcinoma (NPC) occurs with a high incidence in many countries in south-eastern Asia. Chromosomal abnormalities have been commonly found in NPC, but the underlying mechanism is not well understood. We determined mitotic indices, the staining pattern of nuclear DNA and cell cycle profiles of NPC cells in response to treatment with microtubule-disrupting agents, and found that the mitotic checkpoint was defective in two out of five (40%) of the tested NPC cell lines. We also observed that an aberrantly reduced expression of MAD2, one of the key components of mitotic checkpoint, correlated with the loss of checkpoint control. Our findings suggest that a defective mitotic checkpoint characterized by reduced expression of MAD2 contributes to chromosomal instability in NPC.
ISSN0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
ISI Accession Number IDWOS:000166347100021
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWang, X
dc.contributor.authorJin, DY
dc.contributor.authorWong, YC
dc.contributor.authorCheung, ALM
dc.contributor.authorChun, ACS
dc.contributor.authorLo, AKF
dc.contributor.authorLiu, Y
dc.contributor.authorTsao, SW
dc.date.accessioned2010-09-06T05:57:45Z
dc.date.available2010-09-06T05:57:45Z
dc.date.issued2000
dc.description.abstractNasopharyngeal carcinoma (NPC) occurs with a high incidence in many countries in south-eastern Asia. Chromosomal abnormalities have been commonly found in NPC, but the underlying mechanism is not well understood. We determined mitotic indices, the staining pattern of nuclear DNA and cell cycle profiles of NPC cells in response to treatment with microtubule-disrupting agents, and found that the mitotic checkpoint was defective in two out of five (40%) of the tested NPC cell lines. We also observed that an aberrantly reduced expression of MAD2, one of the key components of mitotic checkpoint, correlated with the loss of checkpoint control. Our findings suggest that a defective mitotic checkpoint characterized by reduced expression of MAD2 contributes to chromosomal instability in NPC.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationCarcinogenesis, 2000, v. 21 n. 12, p. 2293-2297 [How to Cite?]
dc.identifier.epage2297
dc.identifier.hkuros56294
dc.identifier.isiWOS:000166347100021
dc.identifier.issn0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
dc.identifier.issue12
dc.identifier.openurl
dc.identifier.pmid11133821
dc.identifier.scopuseid_2-s2.0-0034518709
dc.identifier.spage2293
dc.identifier.urihttp://hdl.handle.net/10722/67732
dc.identifier.volume21
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofCarcinogenesis
dc.relation.referencesReferences in Scopus
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
dc.subject.meshAsia, Southeastern - epidemiology
dc.subject.meshDNA, Neoplasm - analysis
dc.subject.meshDNA-Binding Proteins - analysis - genetics
dc.subject.meshFlow Cytometry
dc.subject.meshHeLa Cells
dc.subject.meshHumans
dc.subject.meshIncidence
dc.subject.meshMitosis - drug effects
dc.subject.meshMitotic Index
dc.subject.meshNasopharyngeal Neoplasms - epidemiology - genetics - pathology
dc.subject.meshNocodazole - pharmacology
dc.subject.meshSmad2 Protein
dc.subject.meshTrans-Activators - analysis - genetics
dc.subject.meshTumor Cells, Cultured
dc.titleCorrelation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong