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Article: Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

TitleInhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma
Authors
KeywordsEsophageal squamous cell carcinoma
Oncogenic expression
Telomerase
Issue Date2007
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2007, v. 19 n. 6, p. 953-960 How to Cite?
AbstractPrevious studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS 50 of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.
Persistent Identifierhttp://hdl.handle.net/10722/67707
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, WKen_HK
dc.contributor.authorChui, CHen_HK
dc.contributor.authorFatima, Sen_HK
dc.contributor.authorKok, SHLen_HK
dc.contributor.authorPak, KCen_HK
dc.contributor.authorOu, TMen_HK
dc.contributor.authorHui, KSen_HK
dc.contributor.authorWong, MMen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorBeh, PSLen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorHo, KPen_HK
dc.contributor.authorChan, ASCen_HK
dc.contributor.authorTang, JCOen_HK
dc.date.accessioned2010-09-06T05:57:32Z-
dc.date.available2010-09-06T05:57:32Z-
dc.date.issued2007en_HK
dc.identifier.citationInternational Journal Of Molecular Medicine, 2007, v. 19 n. 6, p. 953-960en_HK
dc.identifier.issn1107-3756en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67707-
dc.description.abstractPrevious studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongenicity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS 50 of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 μg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.en_HK
dc.languageengen_HK
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_HK
dc.relation.ispartofInternational Journal of Molecular Medicineen_HK
dc.subjectEsophageal squamous cell carcinomaen_HK
dc.subjectOncogenic expressionen_HK
dc.subjectTelomeraseen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarcinoma, Squamous Cell - genetics - pathologyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshEsophageal Neoplasms - genetics - pathologyen_HK
dc.subject.meshFruit - chemistryen_HK
dc.subject.meshGene Expression Regulation, Enzymologic - drug effectsen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshGleditsia - chemistryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiceen_HK
dc.subject.meshNIH 3T3 Cellsen_HK
dc.subject.meshPlant Extracts - pharmacologyen_HK
dc.subject.meshTelomerase - antagonists & inhibitors - genetics - metabolismen_HK
dc.titleInhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1107-3756&volume=19&issue=6&spage=953&epage=960&date=2007&atitle=Inhibitory+effects+of+gleditsia+sinensis+fruit+extract+on+telomerase+activity+and+oncogenic+expression+in+human+esophageal+squamous+cell+carcinomaen_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailBeh, PSL: philipbeh@pathology.hku.hken_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityBeh, PSL=rp00409en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17487429en_HK
dc.identifier.scopuseid_2-s2.0-34548558759en_HK
dc.identifier.hkuros127294en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548558759&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue6en_HK
dc.identifier.spage953en_HK
dc.identifier.epage960en_HK
dc.identifier.isiWOS:000246781000013-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridTang, WK=36678351100en_HK
dc.identifier.scopusauthoridChui, CH=7102093724en_HK
dc.identifier.scopusauthoridFatima, S=8437472300en_HK
dc.identifier.scopusauthoridKok, SHL=8393133400en_HK
dc.identifier.scopusauthoridPak, KC=7102341256en_HK
dc.identifier.scopusauthoridOu, TM=9739783500en_HK
dc.identifier.scopusauthoridHui, KS=7103304771en_HK
dc.identifier.scopusauthoridWong, MM=12777171900en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLam, KY=7403657165en_HK
dc.identifier.scopusauthoridBeh, PSL=6603146797en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridHo, KP=7403581513en_HK
dc.identifier.scopusauthoridChan, ASC=12778594200en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK

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