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Article: Oncogenic properties of a novel gene JK-1 located in chromosome 5p and its overexpression in human esophageal squamous cell carcinoma

TitleOncogenic properties of a novel gene JK-1 located in chromosome 5p and its overexpression in human esophageal squamous cell carcinoma
Authors
KeywordsEsophageal squamous cell carcinoma
Gene overexpression
Transforming capacity
Tumorigenicity
Issue Date2007
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2007, v. 19 n. 6, p. 915-923 How to Cite?
AbstractEsophageal squamous cell carcinoma (ESCC) shows high frequency and mortality in Asian regions, including China. Previous analysis of genomic DNA of ESCC using comparative genomic hybridization indicated that amplification of the chromosome 5p regions is a common event in ESCC cell lines and patient cases of Hong Kong Chinese origin, and the results suggested that the genes located in the chromosome 5p regions may play crucial roles in the molecular pathogenesis of ESCC. Our previous studies on ESCC confirmed the tumorigenic and overexpression properties of a novel gene JS-1 located in chromosome 5p15.2 upstream to δ-catenin. In the present study, another novel gene JK-1 which is located at 5p15.1 downstream to δ-catenin was characterized for its roles in the pathogenesis of ESCC. Thirteen ESCC cell lines and 30 surgical specimens of esophageal tumors were studied for the overexpression of JK-1 using multiplex RT-PCR analysis. The transforming capacity of overexpression of JK-1 was also investigated by transfecting NIH 3T3 and HEK 293 cells with the expression vector cloned with JK-1, followed by the soft agar and foci formation assays. JK-1 was overexpressed in 9/13 (69%) of the ESCC cell lines and 9/30 (30%) of the ESCC patient cases. Both NIH 3T3 and HEK 293 cells acquired the properties of anchorage-dependent and -independent growth when JK-1 was overexpressed. Most significantly, subcutaneous sarcomas were formed in all (3/3) the athymic nude mice after NIH 3T3 cells overexpressing JK-1 were injected subcutaneously. Our results thus indicated that JK-1 is commonly overexpressed in ESCC and has a prominent capacity to transform normal cells. Our overall results thus provide the first evidence that the overexpression of JK-1 and its transforming capacity in normal cells may play a critical role in the molecular pathogenesis of ESCC.
Persistent Identifierhttp://hdl.handle.net/10722/67545
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, WKen_HK
dc.contributor.authorChui, CHen_HK
dc.contributor.authorFatima, Sen_HK
dc.contributor.authorKok, SHLen_HK
dc.contributor.authorPak, KCen_HK
dc.contributor.authorOu, TMen_HK
dc.contributor.authorHui, KSen_HK
dc.contributor.authorWong, MMen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorBeh, PSLen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorChan, ASCen_HK
dc.contributor.authorHo, KPen_HK
dc.contributor.authorTang, JCOen_HK
dc.date.accessioned2010-09-06T05:56:05Z-
dc.date.available2010-09-06T05:56:05Z-
dc.date.issued2007en_HK
dc.identifier.citationInternational Journal Of Molecular Medicine, 2007, v. 19 n. 6, p. 915-923en_HK
dc.identifier.issn1107-3756en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67545-
dc.description.abstractEsophageal squamous cell carcinoma (ESCC) shows high frequency and mortality in Asian regions, including China. Previous analysis of genomic DNA of ESCC using comparative genomic hybridization indicated that amplification of the chromosome 5p regions is a common event in ESCC cell lines and patient cases of Hong Kong Chinese origin, and the results suggested that the genes located in the chromosome 5p regions may play crucial roles in the molecular pathogenesis of ESCC. Our previous studies on ESCC confirmed the tumorigenic and overexpression properties of a novel gene JS-1 located in chromosome 5p15.2 upstream to δ-catenin. In the present study, another novel gene JK-1 which is located at 5p15.1 downstream to δ-catenin was characterized for its roles in the pathogenesis of ESCC. Thirteen ESCC cell lines and 30 surgical specimens of esophageal tumors were studied for the overexpression of JK-1 using multiplex RT-PCR analysis. The transforming capacity of overexpression of JK-1 was also investigated by transfecting NIH 3T3 and HEK 293 cells with the expression vector cloned with JK-1, followed by the soft agar and foci formation assays. JK-1 was overexpressed in 9/13 (69%) of the ESCC cell lines and 9/30 (30%) of the ESCC patient cases. Both NIH 3T3 and HEK 293 cells acquired the properties of anchorage-dependent and -independent growth when JK-1 was overexpressed. Most significantly, subcutaneous sarcomas were formed in all (3/3) the athymic nude mice after NIH 3T3 cells overexpressing JK-1 were injected subcutaneously. Our results thus indicated that JK-1 is commonly overexpressed in ESCC and has a prominent capacity to transform normal cells. Our overall results thus provide the first evidence that the overexpression of JK-1 and its transforming capacity in normal cells may play a critical role in the molecular pathogenesis of ESCC.en_HK
dc.languageengen_HK
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_HK
dc.relation.ispartofInternational Journal of Molecular Medicineen_HK
dc.subjectEsophageal squamous cell carcinomaen_HK
dc.subjectGene overexpressionen_HK
dc.subjectTransforming capacityen_HK
dc.subjectTumorigenicityen_HK
dc.subject.meshAgeden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarcinogenicity Testsen_HK
dc.subject.meshCarcinoma, Squamous Cell - geneticsen_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshChromosomes, Human, Pair 5en_HK
dc.subject.meshEsophageal Neoplasms - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGenes, Neoplasmen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNIH 3T3 Cellsen_HK
dc.subject.meshNeoplasm Proteins - geneticsen_HK
dc.subject.meshOncogenes - physiologyen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleOncogenic properties of a novel gene JK-1 located in chromosome 5p and its overexpression in human esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1107-3756&volume=19&issue=6&spage=915&epage=923&date=2007&atitle=Oncogenic+properties+of+a+novel+gene+JK-1+located+in+chromosome+5p+and+its+overexpression+in+human+esophageal+squamous+cell+carcinomaen_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailBeh, PSL: philipbeh@pathology.hku.hken_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityBeh, PSL=rp00409en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17487424-
dc.identifier.scopuseid_2-s2.0-34548593260en_HK
dc.identifier.hkuros127295en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548593260&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue6en_HK
dc.identifier.spage915en_HK
dc.identifier.epage923en_HK
dc.identifier.isiWOS:000246781000008-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridTang, WK=36678351100en_HK
dc.identifier.scopusauthoridChui, CH=7102093724en_HK
dc.identifier.scopusauthoridFatima, S=8437472300en_HK
dc.identifier.scopusauthoridKok, SHL=8393133400en_HK
dc.identifier.scopusauthoridPak, KC=7102341256en_HK
dc.identifier.scopusauthoridOu, TM=9739783500en_HK
dc.identifier.scopusauthoridHui, KS=7103304771en_HK
dc.identifier.scopusauthoridWong, MM=12777171900en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLam, KY=7403657165en_HK
dc.identifier.scopusauthoridBeh, PSL=6603146797en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridChan, ASC=12778594200en_HK
dc.identifier.scopusauthoridHo, KP=7403581513en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK

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