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Article: Differential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
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TitleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
 
AuthorsFeng, HC1
Tsao, SW1
Ngan, HYS1
Kwan, HS2
Shih, SM2
Xue, WC1
Chiu, PM1
Chan, KW1
Cheung, ANY1
 
KeywordscDNA microarray
Differential gene expression
Hydatidiform mole
Suppression subtractive hybridization
 
Issue Date2006
 
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
 
CitationPlacenta, 2006, v. 27 n. 4-5, p. 521-526 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.placenta.2005.05.005
 
AbstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
 
ISSN0143-4004
2012 Impact Factor: 3.117
2012 SCImago Journal Rankings: 1.177
 
DOIhttp://dx.doi.org/10.1016/j.placenta.2005.05.005
 
ISI Accession Number IDWOS:000237329100023
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFeng, HC
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorNgan, HYS
 
dc.contributor.authorKwan, HS
 
dc.contributor.authorShih, SM
 
dc.contributor.authorXue, WC
 
dc.contributor.authorChiu, PM
 
dc.contributor.authorChan, KW
 
dc.contributor.authorCheung, ANY
 
dc.date.accessioned2010-09-06T05:55:34Z
 
dc.date.available2010-09-06T05:55:34Z
 
dc.date.issued2006
 
dc.description.abstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationPlacenta, 2006, v. 27 n. 4-5, p. 521-526 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.placenta.2005.05.005
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.placenta.2005.05.005
 
dc.identifier.epage526
 
dc.identifier.hkuros109888
 
dc.identifier.isiWOS:000237329100023
 
dc.identifier.issn0143-4004
2012 Impact Factor: 3.117
2012 SCImago Journal Rankings: 1.177
 
dc.identifier.issue4-5
 
dc.identifier.openurl
 
dc.identifier.pmid16026829
 
dc.identifier.scopuseid_2-s2.0-33645080437
 
dc.identifier.spage521
 
dc.identifier.urihttp://hdl.handle.net/10722/67488
 
dc.identifier.volume27
 
dc.languageeng
 
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofPlacenta
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshCase-Control Studies
 
dc.subject.meshChorionic Gonadotropin, beta Subunit, Human - metabolism
 
dc.subject.meshDown-Regulation
 
dc.subject.meshFemale
 
dc.subject.meshGTP-Binding Proteins - metabolism
 
dc.subject.meshGene Expression Profiling - methods
 
dc.subject.meshHumans
 
dc.subject.meshHydatidiform Mole - metabolism
 
dc.subject.meshMiddle Aged
 
dc.subject.meshOligonucleotide Array Sequence Analysis
 
dc.subject.meshOsteopontin
 
dc.subject.meshPlacenta - metabolism
 
dc.subject.meshPolymerase Chain Reaction
 
dc.subject.meshPregnancy
 
dc.subject.meshPregnancy Trimesters
 
dc.subject.meshSequence Analysis, DNA
 
dc.subject.meshSialoglycoproteins - metabolism
 
dc.subject.meshUp-Regulation
 
dc.subjectcDNA microarray
 
dc.subjectDifferential gene expression
 
dc.subjectHydatidiform mole
 
dc.subjectSuppression subtractive hybridization
 
dc.titleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
 
dc.typeArticle
 
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<contributor.author>Shih, SM</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Chinese University of Hong Kong