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Article: Differential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray

TitleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
Authors
KeywordscDNA microarray
Differential gene expression
Hydatidiform mole
Suppression subtractive hybridization
Issue Date2006
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
Citation
Placenta, 2006, v. 27 n. 4-5, p. 521-526 How to Cite?
AbstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67488
ISSN
2014 Impact Factor: 2.710
2014 SCImago Journal Rankings: 1.201
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFeng, HCen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorKwan, HSen_HK
dc.contributor.authorShih, SMen_HK
dc.contributor.authorXue, WCen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-09-06T05:55:34Z-
dc.date.available2010-09-06T05:55:34Z-
dc.date.issued2006en_HK
dc.identifier.citationPlacenta, 2006, v. 27 n. 4-5, p. 521-526en_HK
dc.identifier.issn0143-4004en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67488-
dc.description.abstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placentaen_HK
dc.relation.ispartofPlacentaen_HK
dc.subjectcDNA microarrayen_HK
dc.subjectDifferential gene expressionen_HK
dc.subjectHydatidiform moleen_HK
dc.subjectSuppression subtractive hybridizationen_HK
dc.subject.meshAdulten_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshChorionic Gonadotropin, beta Subunit, Human - metabolismen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGTP-Binding Proteins - metabolismen_HK
dc.subject.meshGene Expression Profiling - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydatidiform Mole - metabolismen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshOsteopontinen_HK
dc.subject.meshPlacenta - metabolismen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPregnancy Trimestersen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSialoglycoproteins - metabolismen_HK
dc.subject.meshUp-Regulationen_HK
dc.titleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarrayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-4004&volume=27&spage=521&epage=526&date=2006&atitle=Differential+gene+expression+identified+in+complete+hydatidiform+mole+by+combining+suppression+subtractive+hybridization+and+cDNA+microarrayen_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailChan, KW:hrmtckw@hku.hken_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.placenta.2005.05.005en_HK
dc.identifier.pmid16026829en_HK
dc.identifier.scopuseid_2-s2.0-33645080437en_HK
dc.identifier.hkuros109888en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645080437&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue4-5en_HK
dc.identifier.spage521en_HK
dc.identifier.epage526en_HK
dc.identifier.isiWOS:000237329100023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridFeng, HC=7401736336en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridKwan, HS=7103369047en_HK
dc.identifier.scopusauthoridShih, SM=12786013600en_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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