Article: Differential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray

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TitleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
AuthorsFeng, HC1
Tsao, SW1
Ngan, HYS1
Kwan, HS2
Shih, SM2
Xue, WC1
Chiu, PM1
Chan, KW1
Cheung, ANY1
KeywordscDNA microarray
Differential gene expression
Hydatidiform mole
Suppression subtractive hybridization
Issue Date2006
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
CitationPlacenta, 2006, v. 27 n. 4-5, p. 521-526 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.placenta.2005.05.005
AbstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
ISSN0143-4004
2011 Impact Factor: 3.693
2011 SCImago Journal Rankings: 0.304
DOIhttp://dx.doi.org/10.1016/j.placenta.2005.05.005
ISI Accession Number IDWOS:000237329100023
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorFeng, HC
dc.contributor.authorTsao, SW
dc.contributor.authorNgan, HYS
dc.contributor.authorKwan, HS
dc.contributor.authorShih, SM
dc.contributor.authorXue, WC
dc.contributor.authorChiu, PM
dc.contributor.authorChan, KW
dc.contributor.authorCheung, ANY
dc.date.accessioned2010-09-06T05:55:34Z
dc.date.available2010-09-06T05:55:34Z
dc.date.issued2006
dc.description.abstractComplete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationPlacenta, 2006, v. 27 n. 4-5, p. 521-526 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.placenta.2005.05.005
dc.identifier.doihttp://dx.doi.org/10.1016/j.placenta.2005.05.005
dc.identifier.epage526
dc.identifier.hkuros109888
dc.identifier.isiWOS:000237329100023
dc.identifier.issn0143-4004
2011 Impact Factor: 3.693
2011 SCImago Journal Rankings: 0.304
dc.identifier.issue4-5
dc.identifier.openurl
dc.identifier.pmid16026829
dc.identifier.scopuseid_2-s2.0-33645080437
dc.identifier.spage521
dc.identifier.urihttp://hdl.handle.net/10722/67488
dc.identifier.volume27
dc.languageeng
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
dc.publisher.placeUnited Kingdom
dc.relation.ispartofPlacenta
dc.relation.referencesReferences in Scopus
dc.subject.meshAdult
dc.subject.meshCase-Control Studies
dc.subject.meshChorionic Gonadotropin, beta Subunit, Human - metabolism
dc.subject.meshDown-Regulation
dc.subject.meshFemale
dc.subject.meshGTP-Binding Proteins - metabolism
dc.subject.meshGene Expression Profiling - methods
dc.subject.meshHumans
dc.subject.meshHydatidiform Mole - metabolism
dc.subject.meshMiddle Aged
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshOsteopontin
dc.subject.meshPlacenta - metabolism
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshPregnancy
dc.subject.meshPregnancy Trimesters
dc.subject.meshSequence Analysis, DNA
dc.subject.meshSialoglycoproteins - metabolism
dc.subject.meshUp-Regulation
dc.subjectcDNA microarray
dc.subjectDifferential gene expression
dc.subjectHydatidiform mole
dc.subjectSuppression subtractive hybridization
dc.titleDifferential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Chinese University of Hong Kong