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Article: Frequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinoma

TitleFrequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinoma
Authors
KeywordsAnchorage-independent growth
DEC1
Esophageal carcinoma
Tumor suppression
Issue Date2008
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2008, v. 122 n. 3, p. 587-594 How to Cite?
AbstractPrevious studies showed that expression of the novel candidate tumor suppressor gene, DEC1 (Deleted in Esophageal Cancer 1), is reduced in esophageal carcinoma and suppresses cancer cell growth in vitro and tumor growth in vivo in nude mice. This study shows that DEC1 gene expression was downregulated in 100% of 16 esophageal squamous cell carcinoma (ESCC) cell lines and 52 and 45%, respectively, of esophageal tumor specimens from Hong Kong and a high-risk ESCC region of Henan, China. Using epitope tagging, the DEC1 protein was localized to both the cytoplasm and nucleus of the cell. In 3D Matrigel culture, no significant difference in colony numbers formed was observed for DEC1 stable transfectants, as compared to vector-alone transfectant controls. However, significantly smaller colony sizes were observed for the DEC1 transfectants. In in vitro cell migration, invasion and soft agar assays of DEC1 transfectants, only the soft agar assay showed statistically significant differences in colony numbers with the vector-alone controls, indicating that DEC1 may be involved in anchorage-independent cell growth. In addition, the global gene expression affected by DEC1 in tumor-suppressive stable transfectants was investigated using cDNA oligonucleotide microarray hybridization. Three candidate genes, TFP1-2, GDF15 and DUSP6, were identified through this approach; they are downregulated in tumor segregants of DEC1 stable transfectants, ESCC cell lines and esophageal tumors and have a potential role in tumor growth and progression. These studies show that DEC1 is involved in esophageal cancer development and help elucidate its functional role in tumor development. © 2007 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67486
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, ACCen_HK
dc.contributor.authorWong, VCLen_HK
dc.contributor.authorLi, CYen_HK
dc.contributor.authorPui, LCen_HK
dc.contributor.authorDaigo, Yen_HK
dc.contributor.authorNakamura, Yen_HK
dc.contributor.authorQi, RZen_HK
dc.contributor.authorMiller, LDen_HK
dc.contributor.authorLiu, ETBen_HK
dc.contributor.authorLi, DWen_HK
dc.contributor.authorLi, JLen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorSai, WTen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2010-09-06T05:55:33Z-
dc.date.available2010-09-06T05:55:33Z-
dc.date.issued2008en_HK
dc.identifier.citationInternational Journal Of Cancer, 2008, v. 122 n. 3, p. 587-594en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67486-
dc.description.abstractPrevious studies showed that expression of the novel candidate tumor suppressor gene, DEC1 (Deleted in Esophageal Cancer 1), is reduced in esophageal carcinoma and suppresses cancer cell growth in vitro and tumor growth in vivo in nude mice. This study shows that DEC1 gene expression was downregulated in 100% of 16 esophageal squamous cell carcinoma (ESCC) cell lines and 52 and 45%, respectively, of esophageal tumor specimens from Hong Kong and a high-risk ESCC region of Henan, China. Using epitope tagging, the DEC1 protein was localized to both the cytoplasm and nucleus of the cell. In 3D Matrigel culture, no significant difference in colony numbers formed was observed for DEC1 stable transfectants, as compared to vector-alone transfectant controls. However, significantly smaller colony sizes were observed for the DEC1 transfectants. In in vitro cell migration, invasion and soft agar assays of DEC1 transfectants, only the soft agar assay showed statistically significant differences in colony numbers with the vector-alone controls, indicating that DEC1 may be involved in anchorage-independent cell growth. In addition, the global gene expression affected by DEC1 in tumor-suppressive stable transfectants was investigated using cDNA oligonucleotide microarray hybridization. Three candidate genes, TFP1-2, GDF15 and DUSP6, were identified through this approach; they are downregulated in tumor segregants of DEC1 stable transfectants, ESCC cell lines and esophageal tumors and have a potential role in tumor growth and progression. These studies show that DEC1 is involved in esophageal cancer development and help elucidate its functional role in tumor development. © 2007 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectAnchorage-independent growthen_HK
dc.subjectDEC1en_HK
dc.subjectEsophageal carcinomaen_HK
dc.subjectTumor suppressionen_HK
dc.subject.meshCarcinoma, Squamous Cell - genetics - metabolism - pathologyen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshEpithelium - metabolism - pathologyen_HK
dc.subject.meshEsophageal Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNeoplasm Invasiveness - pathologyen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshTumor Markers, Biological - genetics - metabolismen_HK
dc.subject.meshTumor Suppressor Proteins - genetics - metabolismen_HK
dc.titleFrequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=122&issue=3&spage=587&epage=594&date=2008&atitle=Frequent+decreased+expression+of+candidate+tumor+suppressor+gene,+DEC1,+and+its+anchorage-independent+growth+properties+and+impact+on+global+gene+expression+in+esophageal+carcinomaen_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailSai, WT: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLung, ML: mlilung@hku.hken_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authoritySai, WT=rp00399en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ijc.23144en_HK
dc.identifier.pmid17943723-
dc.identifier.scopuseid_2-s2.0-37349107523en_HK
dc.identifier.hkuros139681en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37349107523&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue3en_HK
dc.identifier.spage587en_HK
dc.identifier.epage594en_HK
dc.identifier.isiWOS:000252132900014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, ACC=15760220500en_HK
dc.identifier.scopusauthoridWong, VCL=23096631300en_HK
dc.identifier.scopusauthoridLi, CY=36065611200en_HK
dc.identifier.scopusauthoridPui, LC=23095469900en_HK
dc.identifier.scopusauthoridDaigo, Y=7004496677en_HK
dc.identifier.scopusauthoridNakamura, Y=34771694900en_HK
dc.identifier.scopusauthoridQi, RZ=7006273649en_HK
dc.identifier.scopusauthoridMiller, LD=7404985394en_HK
dc.identifier.scopusauthoridLiu, ETB=7202240109en_HK
dc.identifier.scopusauthoridLi, DW=27167922200en_HK
dc.identifier.scopusauthoridLi, JL=8667440000en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridSai, WT=7102813116en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK

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