Article: Frequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinoma

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TitleFrequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinoma
AuthorsLeung, ACC6
Wong, VCL6
Li, CY6
Pui, LC6
Daigo, Y5
Nakamura, Y5
Qi, RZ6
Miller, LD4
Liu, ETB4
Li, DW3
Li, JL1
Law, S2
Sai, WT2
Lung, ML6
KeywordsAnchorage-independent growth
DEC1
Esophageal carcinoma
Tumor suppression
Issue Date2008
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
CitationInternational Journal Of Cancer, 2008, v. 122 n. 3, p. 587-594 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.23144
AbstractPrevious studies showed that expression of the novel candidate tumor suppressor gene, DEC1 (Deleted in Esophageal Cancer 1), is reduced in esophageal carcinoma and suppresses cancer cell growth in vitro and tumor growth in vivo in nude mice. This study shows that DEC1 gene expression was downregulated in 100% of 16 esophageal squamous cell carcinoma (ESCC) cell lines and 52 and 45%, respectively, of esophageal tumor specimens from Hong Kong and a high-risk ESCC region of Henan, China. Using epitope tagging, the DEC1 protein was localized to both the cytoplasm and nucleus of the cell. In 3D Matrigel culture, no significant difference in colony numbers formed was observed for DEC1 stable transfectants, as compared to vector-alone transfectant controls. However, significantly smaller colony sizes were observed for the DEC1 transfectants. In in vitro cell migration, invasion and soft agar assays of DEC1 transfectants, only the soft agar assay showed statistically significant differences in colony numbers with the vector-alone controls, indicating that DEC1 may be involved in anchorage-independent cell growth. In addition, the global gene expression affected by DEC1 in tumor-suppressive stable transfectants was investigated using cDNA oligonucleotide microarray hybridization. Three candidate genes, TFP1-2, GDF15 and DUSP6, were identified through this approach; they are downregulated in tumor segregants of DEC1 stable transfectants, ESCC cell lines and esophageal tumors and have a potential role in tumor growth and progression. These studies show that DEC1 is involved in esophageal cancer development and help elucidate its functional role in tumor development. © 2007 Wiley-Liss, Inc.
ISSN0020-7136
2011 Impact Factor: 5.444
2011 SCImago Journal Rankings: 0.620
DOIhttp://dx.doi.org/10.1002/ijc.23144
ISI Accession Number IDWOS:000252132900014
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLeung, ACC
dc.contributor.authorWong, VCL
dc.contributor.authorLi, CY
dc.contributor.authorPui, LC
dc.contributor.authorDaigo, Y
dc.contributor.authorNakamura, Y
dc.contributor.authorQi, RZ
dc.contributor.authorMiller, LD
dc.contributor.authorLiu, ETB
dc.contributor.authorLi, DW
dc.contributor.authorLi, JL
dc.contributor.authorLaw, S
dc.contributor.authorSai, WT
dc.contributor.authorLung, ML
dc.date.accessioned2010-09-06T05:55:33Z
dc.date.available2010-09-06T05:55:33Z
dc.date.issued2008
dc.description.abstractPrevious studies showed that expression of the novel candidate tumor suppressor gene, DEC1 (Deleted in Esophageal Cancer 1), is reduced in esophageal carcinoma and suppresses cancer cell growth in vitro and tumor growth in vivo in nude mice. This study shows that DEC1 gene expression was downregulated in 100% of 16 esophageal squamous cell carcinoma (ESCC) cell lines and 52 and 45%, respectively, of esophageal tumor specimens from Hong Kong and a high-risk ESCC region of Henan, China. Using epitope tagging, the DEC1 protein was localized to both the cytoplasm and nucleus of the cell. In 3D Matrigel culture, no significant difference in colony numbers formed was observed for DEC1 stable transfectants, as compared to vector-alone transfectant controls. However, significantly smaller colony sizes were observed for the DEC1 transfectants. In in vitro cell migration, invasion and soft agar assays of DEC1 transfectants, only the soft agar assay showed statistically significant differences in colony numbers with the vector-alone controls, indicating that DEC1 may be involved in anchorage-independent cell growth. In addition, the global gene expression affected by DEC1 in tumor-suppressive stable transfectants was investigated using cDNA oligonucleotide microarray hybridization. Three candidate genes, TFP1-2, GDF15 and DUSP6, were identified through this approach; they are downregulated in tumor segregants of DEC1 stable transfectants, ESCC cell lines and esophageal tumors and have a potential role in tumor growth and progression. These studies show that DEC1 is involved in esophageal cancer development and help elucidate its functional role in tumor development. © 2007 Wiley-Liss, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationInternational Journal Of Cancer, 2008, v. 122 n. 3, p. 587-594 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.23144
dc.identifier.doihttp://dx.doi.org/10.1002/ijc.23144
dc.identifier.epage594
dc.identifier.hkuros139681
dc.identifier.isiWOS:000252132900014
dc.identifier.issn0020-7136
2011 Impact Factor: 5.444
2011 SCImago Journal Rankings: 0.620
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid17943723
dc.identifier.scopuseid_2-s2.0-37349107523
dc.identifier.spage587
dc.identifier.urihttp://hdl.handle.net/10722/67486
dc.identifier.volume122
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
dc.publisher.placeUnited States
dc.relation.ispartofInternational Journal of Cancer
dc.relation.referencesReferences in Scopus
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.
dc.subject.meshCarcinoma, Squamous Cell - genetics - metabolism - pathology
dc.subject.meshCell Movement
dc.subject.meshEpithelium - metabolism - pathology
dc.subject.meshEsophageal Neoplasms - genetics - metabolism - pathology
dc.subject.meshFemale
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNeoplasm Invasiveness - pathology
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshTumor Markers, Biological - genetics - metabolism
dc.subject.meshTumor Suppressor Proteins - genetics - metabolism
dc.subjectAnchorage-independent growth
dc.subjectDEC1
dc.subjectEsophageal carcinoma
dc.subjectTumor suppression
dc.titleFrequent decreased expression of candidate tumor suppressor gene, DEC1, and its anchorage-independent growth properties and impact on global gene expression in esophageal carcinoma
dc.typeArticle
Author Affiliations
  1. Yaocun Esophageal Cancer Hospital
  2. The University of Hong Kong
  3. Zhengzhou University
  4. Genome Institute of Singapore
  5. Institute of Medical Science The University of Tokyo
  6. Hong Kong University of Science and Technology