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Article: Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases

TitleMethylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases
Authors
KeywordsCadherin-11
E-cadherin
Gestational trophoblastic diseases
Hydatidiform mole
Methylation
Issue Date2003
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.ijgc.net/
Citation
International Journal Of Gynecological Cancer, 2003, v. 13 n. 6, p. 879-888 How to Cite?
AbstractThe clinical significance of cadherins in gestational trophoblastic diseases (GTD) is not fully understood. In this study, the expression of E-cadherin and cadherin-11 in 12 normal placentas, 32 cases of hydatidiform mole (HM) including 15 complete HMs and 17 partial HMs, and five choriocarcinomas was investigated by immunohistochemistry and correlated with follow-up of HMs. Cases with available frozen blocks were further analyzed by western blot and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). Methylation of E-cadherin was investigated by methylation-specific PCR in six normal first trimester placentas, 19 HMs and their associated deciduas. E-cadherin expression was localized to cytotrophoblast and intermediate trophoblast whereas cadherin-11 was expressed in syncytiotrophoblast, intermediate trophoblast, and decidua. Immunoreactivity of E-cadherin was reduced in choriocarcinoma and complete HM when compared with that in normal first trimester placenta (P < 0.01, P = 0.04). Hypermethylation of E-cadherin was demonstrated in three complete HMs with the lowest level of E-cadherin. Compared with normal first trimester placenta, immunoreactivity of cadherin-11 was higher in complete HM (P=0.02), but lower in choriocarcinoma (P = 0.02). Such differential expression was confirmed by western blot and semiquantitative RT-PCR. No obvious association was observed between the development of persistent trophoblastic disease with the expression of E-cadherin and cadherin-11.
Persistent Identifierhttp://hdl.handle.net/10722/67409
ISSN
2015 Impact Factor: 2.116
2015 SCImago Journal Rankings: 0.830
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXue, WCen_HK
dc.contributor.authorFeng, HCen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorMaccalman, CDen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-09-06T05:54:53Z-
dc.date.available2010-09-06T05:54:53Z-
dc.date.issued2003en_HK
dc.identifier.citationInternational Journal Of Gynecological Cancer, 2003, v. 13 n. 6, p. 879-888en_HK
dc.identifier.issn1048-891Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67409-
dc.description.abstractThe clinical significance of cadherins in gestational trophoblastic diseases (GTD) is not fully understood. In this study, the expression of E-cadherin and cadherin-11 in 12 normal placentas, 32 cases of hydatidiform mole (HM) including 15 complete HMs and 17 partial HMs, and five choriocarcinomas was investigated by immunohistochemistry and correlated with follow-up of HMs. Cases with available frozen blocks were further analyzed by western blot and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). Methylation of E-cadherin was investigated by methylation-specific PCR in six normal first trimester placentas, 19 HMs and their associated deciduas. E-cadherin expression was localized to cytotrophoblast and intermediate trophoblast whereas cadherin-11 was expressed in syncytiotrophoblast, intermediate trophoblast, and decidua. Immunoreactivity of E-cadherin was reduced in choriocarcinoma and complete HM when compared with that in normal first trimester placenta (P < 0.01, P = 0.04). Hypermethylation of E-cadherin was demonstrated in three complete HMs with the lowest level of E-cadherin. Compared with normal first trimester placenta, immunoreactivity of cadherin-11 was higher in complete HM (P=0.02), but lower in choriocarcinoma (P = 0.02). Such differential expression was confirmed by western blot and semiquantitative RT-PCR. No obvious association was observed between the development of persistent trophoblastic disease with the expression of E-cadherin and cadherin-11.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.ijgc.net/en_HK
dc.relation.ispartofInternational Journal of Gynecological Canceren_HK
dc.subjectCadherin-11en_HK
dc.subjectE-cadherinen_HK
dc.subjectGestational trophoblastic diseasesen_HK
dc.subjectHydatidiform moleen_HK
dc.subjectMethylationen_HK
dc.subject.meshAdulten_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCadherins - biosynthesis - metabolismen_HK
dc.subject.meshChoriocarcinoma - genetics - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGestational Trophoblastic Disease - genetics - pathologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydatidiform Mole - genetics - pathologyen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMethylationen_HK
dc.subject.meshPlacenta - physiologyen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshUterine Neoplasms - genetics - pathologyen_HK
dc.titleMethylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseasesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1048-891X&volume=13&issue=6&spage=879&epage=888&date=2003&atitle=Methylation+status+and+expression+of+E-cadherin+and+cadherin-11+in+gestational+trophoblastic+diseasesen_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1525-1438.2003.13400.xen_HK
dc.identifier.pmid14675328-
dc.identifier.scopuseid_2-s2.0-1242277423en_HK
dc.identifier.hkuros86087en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1242277423&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue6en_HK
dc.identifier.spage879en_HK
dc.identifier.epage888en_HK
dc.identifier.isiWOS:000187184800022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridFeng, HC=7401736336en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridMaccalman, CD=7003694524en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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