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Conference Paper: Functional characterization of GRB7 and its variant, GRB7v, in ovarian cancer

TitleFunctional characterization of GRB7 and its variant, GRB7v, in ovarian cancer
Authors
Issue Date2009
PublisherAmerican Association for Cancer Research
Citation
The American Association for Cancer Research Annual Meeting, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 3333 How to Cite?
AbstractOvarian cancer is one of the most lethal gynecological malignancies for female overall in the world. Most of ovarian cancer cases have poor prognosis and patients die at late stage. Therefore, understanding the molecular mechanisms in the development of ovarian cancer through identification and characterization of oncogenes and tumor suppressor genes will help discovery of novel targets for therapies. We have recently identified Growth factor Receptor Bound protein 7 (GRB7) and its variant GRB7v (deletion of SH2 domain) overexpressed in ovarian cancer cells and clinical samples by quantitative RT-PCR analysis. Clinicopathological correlation revealed that the overexpressed GRB7 (P=0.019) and GRB7v (P=0.018) were significantly correlated with high grade ovarian cancer. In addition, the overexpressed GRB7 was also associated with clear cell subtype of ovarian cancer (P=0.024). GRB7 is an adaptor protein which plays an important role in mediating signal transductions from multiple cell surface receptors to various downstream signaling pathways. We thus hypothesized that the overexpressed GRB7 and GRB7v may play important roles in the development and progression of ovarian cancer. Subcellular localization studies using immunofluorescent analysis showed that GRB7 and GRB7v localized mainly in cytoplasm, consistent with their roles as activated receptor binding proteins. Functionally, we demonstrated that the ectopic overexpression of GRB7 promoted cell migration and invasion, whereas enforced expression of GRB7v increased cell proliferation instead of promoting cell migration and invasion. This indicates that GRB7 and GRB7v function differentially and may be involved in different signaling pathways in the pathogenesis of high grade ovarian cancer cells. Further investigations of the functions and the underlying molecular mechanisms of GRB7 and GRB7v in ovarian cancer are warranted.
Persistent Identifierhttp://hdl.handle.net/10722/63539
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorChan, DWen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-07-13T04:25:58Z-
dc.date.available2010-07-13T04:25:58Z-
dc.date.issued2009en_HK
dc.identifier.citationThe American Association for Cancer Research Annual Meeting, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 3333-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/63539-
dc.description.abstractOvarian cancer is one of the most lethal gynecological malignancies for female overall in the world. Most of ovarian cancer cases have poor prognosis and patients die at late stage. Therefore, understanding the molecular mechanisms in the development of ovarian cancer through identification and characterization of oncogenes and tumor suppressor genes will help discovery of novel targets for therapies. We have recently identified Growth factor Receptor Bound protein 7 (GRB7) and its variant GRB7v (deletion of SH2 domain) overexpressed in ovarian cancer cells and clinical samples by quantitative RT-PCR analysis. Clinicopathological correlation revealed that the overexpressed GRB7 (P=0.019) and GRB7v (P=0.018) were significantly correlated with high grade ovarian cancer. In addition, the overexpressed GRB7 was also associated with clear cell subtype of ovarian cancer (P=0.024). GRB7 is an adaptor protein which plays an important role in mediating signal transductions from multiple cell surface receptors to various downstream signaling pathways. We thus hypothesized that the overexpressed GRB7 and GRB7v may play important roles in the development and progression of ovarian cancer. Subcellular localization studies using immunofluorescent analysis showed that GRB7 and GRB7v localized mainly in cytoplasm, consistent with their roles as activated receptor binding proteins. Functionally, we demonstrated that the ectopic overexpression of GRB7 promoted cell migration and invasion, whereas enforced expression of GRB7v increased cell proliferation instead of promoting cell migration and invasion. This indicates that GRB7 and GRB7v function differentially and may be involved in different signaling pathways in the pathogenesis of high grade ovarian cancer cells. Further investigations of the functions and the underlying molecular mechanisms of GRB7 and GRB7v in ovarian cancer are warranted.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Research-
dc.titleFunctional characterization of GRB7 and its variant, GRB7v, in ovarian canceren_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWang, Y: yajunwang2002@yahoo.comen_HK
dc.identifier.emailChan, DW: dwchan@hkucc.hku.hken_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityChan, DW=rp00543en_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.hkuros158041en_HK

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