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Conference Paper: Occult hepatitis B virus reinfection in liver transplant recipient

TitleOccult hepatitis B virus reinfection in liver transplant recipient
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 14th Annual Joint International Congress of the International Liver Transplantation Society (ILTS), European Liver and Intestine Transplant Association (ELITA), and European Society for Organ Transplantation (ESOT), Paris, France, 9-12 July 2008. In Liver Transplantation, 2008, v. 14 suppl. 1, p. S237, abstract no. 594 How to Cite?
AbstractBACKGROUND AND AIM: Nucleoside analogue prophylaxis can prevent recurrent hepatitis B virus (HBV) infection with persistent clearance of serum hepatitis B surface antigen (HBsAg) after liver transplantation (OLT). Despite the absence of any serologic marker of HBV recurrence, however, it remains unknown whether there is occult reinfection in the liver graft. We aimed to detect and quantify the presence of intrahepatic HBV DNA in the liver grafts of patients who remain seronegative for HBsAg for more than 1 year after liver transplantation. MATERIALS AND METHODS: Liver biopsy and blood samples were obtained from 23 patients who had been receiving nucleoside analogue prophylaxis alone and remained persistently seronegative for HBsAg (medium, 40.6 months; range, 10.7 to 117.8 months) after OLT for chronic hepatitis B. Liver biopsies were stained for HBsAg and HBcAg by immunohistochemical staining. Quantitative polymerase chain reaction was performed to detect and quantify total and covalently closed circular (ccc) HBV DNA in the liver (lowest detection limit, 10 copies/mL ), serum and PBMC. Direct sequencing was used for HBV quasispecies screening. RESULTS: All serum samples were negative for HBV DNA but total &/or cccHBV DNA were detectable in the liver graft of 20 of 23 patients. It was positive in 17 of 20 liver grafts from donors seropositive for anti-HBc as compared to 1 of 3 from anti-HBc-negative donors (p=0.043). Pre-transplant liver biopsy specimen was available from 6 donors and intrahepatic HBV DNA was detectable in all 4 who were seropositive for anti-HBc. Comparison of the HBV DNA sequence from patients’ pre-transplant serum and posttransplant liver biopsy as well as the donor’s pre-transplant liver biopsy …
DescriptionConcurrent Session: Hepatitis 2
This journal suppl. entitled: Abstracts: The International Liver Transplantation Society: 14th Annual International Congress, July 9–12, 2008, Paris, France
Persistent Identifierhttp://hdl.handle.net/10722/62430
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorCheung, CKYen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorLau, Gen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorWong, Jen_HK
dc.date.accessioned2010-07-13T04:01:03Z-
dc.date.available2010-07-13T04:01:03Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 14th Annual Joint International Congress of the International Liver Transplantation Society (ILTS), European Liver and Intestine Transplant Association (ELITA), and European Society for Organ Transplantation (ESOT), Paris, France, 9-12 July 2008. In Liver Transplantation, 2008, v. 14 suppl. 1, p. S237, abstract no. 594-
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/62430-
dc.descriptionConcurrent Session: Hepatitis 2-
dc.descriptionThis journal suppl. entitled: Abstracts: The International Liver Transplantation Society: 14th Annual International Congress, July 9–12, 2008, Paris, France-
dc.description.abstractBACKGROUND AND AIM: Nucleoside analogue prophylaxis can prevent recurrent hepatitis B virus (HBV) infection with persistent clearance of serum hepatitis B surface antigen (HBsAg) after liver transplantation (OLT). Despite the absence of any serologic marker of HBV recurrence, however, it remains unknown whether there is occult reinfection in the liver graft. We aimed to detect and quantify the presence of intrahepatic HBV DNA in the liver grafts of patients who remain seronegative for HBsAg for more than 1 year after liver transplantation. MATERIALS AND METHODS: Liver biopsy and blood samples were obtained from 23 patients who had been receiving nucleoside analogue prophylaxis alone and remained persistently seronegative for HBsAg (medium, 40.6 months; range, 10.7 to 117.8 months) after OLT for chronic hepatitis B. Liver biopsies were stained for HBsAg and HBcAg by immunohistochemical staining. Quantitative polymerase chain reaction was performed to detect and quantify total and covalently closed circular (ccc) HBV DNA in the liver (lowest detection limit, 10 copies/mL ), serum and PBMC. Direct sequencing was used for HBV quasispecies screening. RESULTS: All serum samples were negative for HBV DNA but total &/or cccHBV DNA were detectable in the liver graft of 20 of 23 patients. It was positive in 17 of 20 liver grafts from donors seropositive for anti-HBc as compared to 1 of 3 from anti-HBc-negative donors (p=0.043). Pre-transplant liver biopsy specimen was available from 6 donors and intrahepatic HBV DNA was detectable in all 4 who were seropositive for anti-HBc. Comparison of the HBV DNA sequence from patients’ pre-transplant serum and posttransplant liver biopsy as well as the donor’s pre-transplant liver biopsy …-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantation-
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.titleOccult hepatitis B virus reinfection in liver transplant recipienten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailCheung, CKY: cindycky@HKUCC-COM.hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailLau, G: gkklau@netvigator.comen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.21569-
dc.identifier.hkuros151542en_HK
dc.identifier.hkuros165538-
dc.identifier.volume14-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS237, abstract no. 594-
dc.identifier.epageS237, abstract no. 594-

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