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Conference Paper: Matrix remodeling during intervertebral disc growth and degeneration detected by multichromatic fast staining

TitleMatrix remodeling during intervertebral disc growth and degeneration detected by multichromatic fast staining
Authors
KeywordsDegeneration
Growth
Intervertebral disc
Matrix
Remodeling
Staining and labeling
Issue Date2009
PublisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org
Citation
The 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV., 22-24 February 2009. In Journal of Histochemistry and Cytochemistry, 2009, v. 57 n. 3, p. 249-256 How to Cite?
AbstractVarious imaging techniques have been used to assess degeneration of the intervertebral disc, including many histological methods, but cartilage-oriented histological stains do not clearly show the comparatively complex structures of the disc. In addition, there is no integrated method to assess efficiently both the compartmental organization and matrix composition in disc samples. In this study, a novel histological method, termed FAST staining, has been developed to investigate disc growth and degeneration by sequen- tial staining with fast green, Alcian blue, Safranin-O, and tartrazine to generate multi- chromatic histological profiles (FAST profiles). This identifies the major compartments of the vertebra-disc region, including the cartilaginous endplate and multiple zones of the annulus fibrosus, by specific FAST profile patterns. A disc degeneration model in rabbit established using a previously described puncture method showed gradual but profound alteration of the FAST profile during disc degeneration, supporting continual alteration of glycosaminoglycan. Changes of the FAST profile pattern in the nucleus pulposus and an- nulus fibrosus of the postnatal mouse spine suggested matrix remodeling activity during the growth of intervertebral discs. In summary, we developed an effective staining method capable of defining intervertebral disc compartments in detail and showing matrix remodel- ing events within the disc. The FAST staining method may be used to develop a histopatho- logical grading system to evaluate disc degeneration or malformation. © The Histochemical Society, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/61626
ISSN
2015 Impact Factor: 1.953
2015 SCImago Journal Rankings: 1.187
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
University Grants Committee of the Hong Kong Special Administrative Region of ChinaHKU7496/OSM and AoE 04/04
AOSPLNEAOSBRC-07-02
Funding Information:

We thank Prof. Koichi Masuda and his team in Rush Medical Center, Chicago, IL, for excellent technical advice on the generation of rabbit disc degeneration model.

References

 

DC FieldValueLanguage
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorChan, WCWen_HK
dc.contributor.authorHung, SCen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorChan, Den_HK
dc.date.accessioned2010-07-13T03:43:45Z-
dc.date.available2010-07-13T03:43:45Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV., 22-24 February 2009. In Journal of Histochemistry and Cytochemistry, 2009, v. 57 n. 3, p. 249-256-
dc.identifier.issn0022-1554en_HK
dc.identifier.urihttp://hdl.handle.net/10722/61626-
dc.description.abstractVarious imaging techniques have been used to assess degeneration of the intervertebral disc, including many histological methods, but cartilage-oriented histological stains do not clearly show the comparatively complex structures of the disc. In addition, there is no integrated method to assess efficiently both the compartmental organization and matrix composition in disc samples. In this study, a novel histological method, termed FAST staining, has been developed to investigate disc growth and degeneration by sequen- tial staining with fast green, Alcian blue, Safranin-O, and tartrazine to generate multi- chromatic histological profiles (FAST profiles). This identifies the major compartments of the vertebra-disc region, including the cartilaginous endplate and multiple zones of the annulus fibrosus, by specific FAST profile patterns. A disc degeneration model in rabbit established using a previously described puncture method showed gradual but profound alteration of the FAST profile during disc degeneration, supporting continual alteration of glycosaminoglycan. Changes of the FAST profile pattern in the nucleus pulposus and an- nulus fibrosus of the postnatal mouse spine suggested matrix remodeling activity during the growth of intervertebral discs. In summary, we developed an effective staining method capable of defining intervertebral disc compartments in detail and showing matrix remodel- ing events within the disc. The FAST staining method may be used to develop a histopatho- logical grading system to evaluate disc degeneration or malformation. © The Histochemical Society, Inc.en_HK
dc.languageengen_HK
dc.publisherHistochemical Society. The Journal's web site is located at http://intl.jhc.orgen_HK
dc.relation.ispartofJournal of Histochemistry and Cytochemistryen_HK
dc.subjectDegenerationen_HK
dc.subjectGrowthen_HK
dc.subjectIntervertebral discen_HK
dc.subjectMatrixen_HK
dc.subjectRemodelingen_HK
dc.subjectStaining and labelingen_HK
dc.subject.meshExtracellular Matrix - pathology-
dc.subject.meshIntervertebral Disc - growth and development - pathology-
dc.subject.meshPhenazines-
dc.subject.meshSpinal Diseases - pathology-
dc.subject.meshTartrazine-
dc.titleMatrix remodeling during intervertebral disc growth and degeneration detected by multichromatic fast stainingen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1369/jhc.2008.952184en_HK
dc.identifier.pmid19001641-
dc.identifier.pmcidPMC2664937-
dc.identifier.scopuseid_2-s2.0-62649088064en_HK
dc.identifier.hkuros160667en_HK
dc.identifier.hkuros160660-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62649088064&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume57en_HK
dc.identifier.issue3en_HK
dc.identifier.spage249en_HK
dc.identifier.epage256en_HK
dc.identifier.isiWOS:000263530800007-
dc.publisher.placeUnited Statesen_HK
dc.description.otherThe 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV., 22-24 February 2009. In Journal of Histochemistry and Cytochemistry, 2009, v. 57 n. 3, p. 249-256-
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridChan, WCW=24545687600en_HK
dc.identifier.scopusauthoridHung, SC=35757582000en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK

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