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Conference Paper: Intervertebral Disc Cryopreservation
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TitleIntervertebral Disc Cryopreservation
 
AuthorsChan, CW
Leung, YL
Lu, WW
Chan, D
Luk, KDK
Cheung, KMC
 
Issue Date2008
 
PublisherInternational Society of Orthopaedic Surgery and Traumatology.
 
CitationSICOT/SIROT 2008 XXIV Triennial World Congress, Hong Kong, 24-28 August 2008, p. abstract no. 17964 [How to Cite?]
 
AbstractCryopreserved allogeneic intervertebral disc (IVD) transplantation has been performed clinically for treating disc degeneration. However, the transplanted allograft has undergone progressive degeneration 1. Conventional disc cryopreservation method has been shown to greatly decrease the disc cell viability and hence may be a cause of the degeneration. In this study, we aim to search for a freeze-thaw protocol that is able to retain a maximal cell viability and metabolic activity in the cryopreserved IVD. Porcine lumbar IVDs with endplates were harvested and cryopreserved in various freezing medium 1) 45% Cryo-protective agents (CPAs) (DMSO (dimethylsulfoxide) + propylene glycol), 2) 20% CPAs (DMSO + glycerol), 3) 10% DMSO with synthetic ice-blocker , 4) 10% DMSO, or 5) without CPA. IVDs were incubated at 4°C for 1 hour, freezed at -80°C overnight, and then stored in liquid nitrogen. The frozen IVDs were thawed quickly at 37°C for analysis. Cell prol iferation and proteoglycan production rate were evaluated using Alarma blue and 35S-sulphate incorporation respectively. The distribution of live and dead cells was determined microscopically using a LIVD/DEAD staining. IVD cryopreserved in freezing medium composed of 45% CPAs showed higher disc cell viability compared with that in 10% DMSO. Cell viability was 50% of the fresh IVD tissue when cryopreserved in 45% CPAs but merely 20% when cryopreserved with 10% DMSO. Combination of DMSO and propylene glycol as CPAs for IVD cryopreservation has an advantage over conventional cryopreservation protocol in maintaining disc cell viability. Reference: 1. Ruan D, et al. Lancet 2007; 369:993-9.
 
DescriptionSession: Spine: basic science
Oral presentation
 
DC FieldValue
dc.contributor.authorChan, CW
 
dc.contributor.authorLeung, YL
 
dc.contributor.authorLu, WW
 
dc.contributor.authorChan, D
 
dc.contributor.authorLuk, KDK
 
dc.contributor.authorCheung, KMC
 
dc.date.accessioned2010-07-13T03:43:40Z
 
dc.date.available2010-07-13T03:43:40Z
 
dc.date.issued2008
 
dc.description.abstractCryopreserved allogeneic intervertebral disc (IVD) transplantation has been performed clinically for treating disc degeneration. However, the transplanted allograft has undergone progressive degeneration 1. Conventional disc cryopreservation method has been shown to greatly decrease the disc cell viability and hence may be a cause of the degeneration. In this study, we aim to search for a freeze-thaw protocol that is able to retain a maximal cell viability and metabolic activity in the cryopreserved IVD. Porcine lumbar IVDs with endplates were harvested and cryopreserved in various freezing medium 1) 45% Cryo-protective agents (CPAs) (DMSO (dimethylsulfoxide) + propylene glycol), 2) 20% CPAs (DMSO + glycerol), 3) 10% DMSO with synthetic ice-blocker , 4) 10% DMSO, or 5) without CPA. IVDs were incubated at 4°C for 1 hour, freezed at -80°C overnight, and then stored in liquid nitrogen. The frozen IVDs were thawed quickly at 37°C for analysis. Cell prol iferation and proteoglycan production rate were evaluated using Alarma blue and 35S-sulphate incorporation respectively. The distribution of live and dead cells was determined microscopically using a LIVD/DEAD staining. IVD cryopreserved in freezing medium composed of 45% CPAs showed higher disc cell viability compared with that in 10% DMSO. Cell viability was 50% of the fresh IVD tissue when cryopreserved in 45% CPAs but merely 20% when cryopreserved with 10% DMSO. Combination of DMSO and propylene glycol as CPAs for IVD cryopreservation has an advantage over conventional cryopreservation protocol in maintaining disc cell viability. Reference: 1. Ruan D, et al. Lancet 2007; 369:993-9.
 
dc.descriptionSession: Spine: basic science
 
dc.descriptionOral presentation
 
dc.identifier.citationSICOT/SIROT 2008 XXIV Triennial World Congress, Hong Kong, 24-28 August 2008, p. abstract no. 17964 [How to Cite?]
 
dc.identifier.epageabstract no. 17964
 
dc.identifier.hkuros166115
 
dc.identifier.spageabstract no. 17964
 
dc.identifier.urihttp://hdl.handle.net/10722/61622
 
dc.languageeng
 
dc.publisherInternational Society of Orthopaedic Surgery and Traumatology.
 
dc.publisher.placeFrance
 
dc.relation.ispartofSICOT/SIROT World Congress
 
dc.titleIntervertebral Disc Cryopreservation
 
dc.typeConference_Paper
 
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<contributor.author>Leung, YL</contributor.author>
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<contributor.author>Chan, D</contributor.author>
<contributor.author>Luk, KDK</contributor.author>
<contributor.author>Cheung, KMC</contributor.author>
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<date.available>2010-07-13T03:43:40Z</date.available>
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<description.abstract>Cryopreserved allogeneic intervertebral disc (IVD) transplantation has been performed clinically for treating disc degeneration. However, the transplanted allograft has undergone progressive degeneration 1. Conventional disc cryopreservation method has been shown to greatly decrease the disc cell viability and hence may be a cause of the degeneration. In this study, we aim to search for a freeze-thaw protocol that is able to retain a maximal cell viability and metabolic activity in the cryopreserved IVD. Porcine lumbar IVDs with endplates were harvested and cryopreserved in various freezing medium 1) 45% Cryo-protective agents (CPAs) (DMSO (dimethylsulfoxide) + propylene glycol), 2) 20% CPAs (DMSO + glycerol), 3) 10% DMSO with synthetic ice-blocker , 4) 10% DMSO, or 5) without CPA. IVDs were incubated at 4&#176;C for 1 hour, freezed at -80&#176;C overnight, and then stored in liquid nitrogen. The frozen IVDs were thawed quickly at 37&#176;C for analysis. Cell prol iferation and proteoglycan production rate were evaluated using Alarma blue and 35S-sulphate incorporation respectively. The distribution of live and dead cells was determined microscopically using a LIVD/DEAD staining. IVD cryopreserved in freezing medium composed of 45% CPAs showed higher disc cell viability compared with that in 10% DMSO. Cell viability was 50% of the fresh IVD tissue when cryopreserved in 45% CPAs but merely 20% when cryopreserved with 10% DMSO. Combination of DMSO and propylene glycol as CPAs for IVD cryopreservation has an advantage over conventional cryopreservation protocol in maintaining disc cell viability. Reference: 1. Ruan D, et al. Lancet 2007; 369:993-9.</description.abstract>
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