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Conference Paper: In Vivo Chondrocytic Differentiation of the Allogenic Stem Cells in the Murine Intervertebral Disc

TitleIn Vivo Chondrocytic Differentiation of the Allogenic Stem Cells in the Murine Intervertebral Disc
Authors
Issue Date2008
PublisherInternational Society of Orthopaedic Surgery and Traumatology.
Citation
The SICOT/SIROT 24th Triennial World Congress, Hong Kong, 24-28 August 2008. In Oral Presentation Abstracts, p. 335, abstract no. 17870 How to Cite?
Abstract
Various kinds of biological therapeutic methods have been used to treat DDD. Some in vitro studies have shown that bone marrow derived mesenchymal stem cells (BMSC) could differentiate toward a nucleus-pulposus (NP) phenotype. Some in vivo studies also showed that the autograft of BMSC could regenerate the rabbit disc. However, the fate and differentiation status of injected cells are still not very clear. This is partly due to the lack of a stable marker of BMSC; we overcome this by isolating BMSC from green fluorescent protein (GFP) mice. The viable cells can express stable green fluorescent protein. We have also established a mouse model of disc degeneration through annular puncturing method under microscopic guidance. BMSC were injected into the murine discs two weeks after the degeneration was induced by annular puncture. Serials analysis including radiograph, histology, immunostaining and biochemical analysis were performed at 4 and 24 weeks after the transplantation. Radiograph analysis and histological grading confirmed that progressive degeneration of the discs was arrested after the transplantation of the BMSC. Gene expression analysis showed that the proteoglycan genes were upregulated significantly in the regenerated NP. Double staining method suggested the in vivo chondrocytic differentiation of the BMSC in the regeneration process. Our study showed that the allogenic BMSC could arrest the degeneration of the murine discs and increase the extracellular matrix in the NP region. The accumulation of the ECM is caused by the in vivo chondrocytic differentiation of the stem cells.
DescriptionSession: Spine: basic science
Persistent Identifierhttp://hdl.handle.net/10722/61605

 

DC FieldValueLanguage
dc.contributor.authorYang, Fen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.date.accessioned2010-07-13T03:43:20Z-
dc.date.available2010-07-13T03:43:20Z-
dc.date.issued2008en_HK
dc.identifier.citationThe SICOT/SIROT 24th Triennial World Congress, Hong Kong, 24-28 August 2008. In Oral Presentation Abstracts, p. 335, abstract no. 17870-
dc.identifier.urihttp://hdl.handle.net/10722/61605-
dc.descriptionSession: Spine: basic science-
dc.descriptionOral presentation-
dc.description.abstractVarious kinds of biological therapeutic methods have been used to treat DDD. Some in vitro studies have shown that bone marrow derived mesenchymal stem cells (BMSC) could differentiate toward a nucleus-pulposus (NP) phenotype. Some in vivo studies also showed that the autograft of BMSC could regenerate the rabbit disc. However, the fate and differentiation status of injected cells are still not very clear. This is partly due to the lack of a stable marker of BMSC; we overcome this by isolating BMSC from green fluorescent protein (GFP) mice. The viable cells can express stable green fluorescent protein. We have also established a mouse model of disc degeneration through annular puncturing method under microscopic guidance. BMSC were injected into the murine discs two weeks after the degeneration was induced by annular puncture. Serials analysis including radiograph, histology, immunostaining and biochemical analysis were performed at 4 and 24 weeks after the transplantation. Radiograph analysis and histological grading confirmed that progressive degeneration of the discs was arrested after the transplantation of the BMSC. Gene expression analysis showed that the proteoglycan genes were upregulated significantly in the regenerated NP. Double staining method suggested the in vivo chondrocytic differentiation of the BMSC in the regeneration process. Our study showed that the allogenic BMSC could arrest the degeneration of the murine discs and increase the extracellular matrix in the NP region. The accumulation of the ECM is caused by the in vivo chondrocytic differentiation of the stem cells.-
dc.languageengen_HK
dc.publisherInternational Society of Orthopaedic Surgery and Traumatology.-
dc.relation.ispartofSICOT/SIROT 24th Triennial World Congress 2008-
dc.titleIn Vivo Chondrocytic Differentiation of the Allogenic Stem Cells in the Murine Intervertebral Discen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailLu, WW: wwlu@hku.hken_HK
dc.identifier.emailLuk, KDK: hrmoldk@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros166114en_HK
dc.identifier.spage335-
dc.identifier.epage335-
dc.publisher.placeFrance-

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