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Article: The inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinoma

TitleThe inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinoma
Authors
KeywordsAnti-proliferation
Cyclooxygenase-2
Esophaegal squamous cell carcinoma
Gene over-expression
Issue Date2009
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2009, v. 23 n. 1, p. 121-129 How to Cite?
AbstractThe anti-cancer effects of the anomalous fruit extract of Gleditsia sinensis (GSE) attributed to its apoptotic activity, telomerase inhibition and anti-angiogenesis in a panel of solid and non-solid tumor cell lines including esophageal squamous cell carcinoma (ESCC) have been intensively investigated by us in previous studies. Cyclooxygenase-2 (COX-2) has been well described as another promising target of cancer therapy for ESCC, and novel therapeutic agents are still being sought which target COX-2 expression. However, the anti-cancer effect of GSE through the suppression of COX-2 expression has not been previously investigated. In the present study, the anti-cancer effects of GSE on eight ESCC cell lines (KYSE 30, KYSE 150, KYSE 450, KYSE 510, KYSE 520, HKESC-3, HKESC-4 and SLMT-1) of Chinese and Japanese origins were first studied by MTS cytotoxicity assays. The effects of GSE on COX-2 expression levels and on the housekeeping form COX-1 were also investigated by multiplex RT-PCR analysis. Moreover, the anti-proliferative effect of GSE on KYSE 510 was also studied by anchorage-independent clonogenicity assay in soft agar. The results showed that GSE induced a dose- and time-dependent cytotoxicity on all of the eight ESCC cell lines and caused positive anti-proliferative action on KYSE 510 in the anchorage-independent clonogenicity assay, suggesting that GSE suppressed the in vitro growth of the ESCC cell lines. More importantly, the MRNA expression levels of COX-2, but not COX-1, in all of the ESCC cell lines were suppressed by GSE in a dose-dependent fashion. The overall results of the present study show that the anti-cancer effect of GSE on the ESCC cell lines is associated with the suppression of COX-2 expression, but not COX-1. Our findings also open a new chapter for the future advancement of GSE as a novel anticancer agent or as an adjuvant of traditional cancer treatments.
Persistent Identifierhttp://hdl.handle.net/10722/60583
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID
Funding AgencyGrant Number
PolyU General Research FundG-U255
PolyU Niche Area FundBB65
Niche Area FundBB8P
Funding Information:

We acknowledge the donation of Mr. K.C. Lo for setting up the Lo Ka Chung Centre for Natural Anti-Cancer Drug Development and for supporting the research described in this report. The present study was also supported by the PolyU General Research Fund (grant no. G-U255), PolyU Niche Area Fund (grant no. BB65) for the project 'Novel Anti-Cancer Small Molecule Drug R&D' and the Niche Area Fund for the development of traditional Chinese medicine (grant no. BB8P). This research study is also part of the M.Phil. study of K.C. Pak.

References

 

DC FieldValueLanguage
dc.contributor.authorPak, KCen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorTang, JCOen_HK
dc.date.accessioned2010-05-31T04:14:09Z-
dc.date.available2010-05-31T04:14:09Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Molecular Medicine, 2009, v. 23 n. 1, p. 121-129en_HK
dc.identifier.issn1107-3756en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60583-
dc.description.abstractThe anti-cancer effects of the anomalous fruit extract of Gleditsia sinensis (GSE) attributed to its apoptotic activity, telomerase inhibition and anti-angiogenesis in a panel of solid and non-solid tumor cell lines including esophageal squamous cell carcinoma (ESCC) have been intensively investigated by us in previous studies. Cyclooxygenase-2 (COX-2) has been well described as another promising target of cancer therapy for ESCC, and novel therapeutic agents are still being sought which target COX-2 expression. However, the anti-cancer effect of GSE through the suppression of COX-2 expression has not been previously investigated. In the present study, the anti-cancer effects of GSE on eight ESCC cell lines (KYSE 30, KYSE 150, KYSE 450, KYSE 510, KYSE 520, HKESC-3, HKESC-4 and SLMT-1) of Chinese and Japanese origins were first studied by MTS cytotoxicity assays. The effects of GSE on COX-2 expression levels and on the housekeeping form COX-1 were also investigated by multiplex RT-PCR analysis. Moreover, the anti-proliferative effect of GSE on KYSE 510 was also studied by anchorage-independent clonogenicity assay in soft agar. The results showed that GSE induced a dose- and time-dependent cytotoxicity on all of the eight ESCC cell lines and caused positive anti-proliferative action on KYSE 510 in the anchorage-independent clonogenicity assay, suggesting that GSE suppressed the in vitro growth of the ESCC cell lines. More importantly, the MRNA expression levels of COX-2, but not COX-1, in all of the ESCC cell lines were suppressed by GSE in a dose-dependent fashion. The overall results of the present study show that the anti-cancer effect of GSE on the ESCC cell lines is associated with the suppression of COX-2 expression, but not COX-1. Our findings also open a new chapter for the future advancement of GSE as a novel anticancer agent or as an adjuvant of traditional cancer treatments.en_HK
dc.languageengen_HK
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_HK
dc.relation.ispartofInternational Journal of Molecular Medicineen_HK
dc.subjectAnti-proliferationen_HK
dc.subjectCyclooxygenase-2en_HK
dc.subjectEsophaegal squamous cell carcinomaen_HK
dc.subjectGene over-expressionen_HK
dc.titleThe inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1107-3756&volume=23&issue=1&spage=121&epage=129&date=2009&atitle=The+inhibitory+effect+of+Gleditsia+sinensis+on+cyclooxygenase-2+expression+in+human+esophageal+squamous+cell+carcinomaen_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3892/ijmm_00000109en_HK
dc.identifier.scopuseid_2-s2.0-61749090021en_HK
dc.identifier.hkuros154280en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61749090021&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue1en_HK
dc.identifier.spage121en_HK
dc.identifier.epage129en_HK
dc.identifier.isiWOS:000262014000015-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridPak, KC=7102341256en_HK
dc.identifier.scopusauthoridLam, KY=7403657165en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK

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