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Article: P63 expression in gestational trophoblastic disease: Correlation with proliferation and apoptotic dynamics

TitleP63 expression in gestational trophoblastic disease: Correlation with proliferation and apoptotic dynamics
Authors
KeywordsGestational trophoblastic disease
Gestational trophoblastic neoplasia
P63
Issue Date2009
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.com
Citation
International Journal Of Gynecological Pathology, 2009, v. 28 n. 2, p. 172-178 How to Cite?
Abstractp63 regulates cell growth and differentiation and contributes to tumorigenesis through its complex isoforms. Gestational trophoblastic disease encompasses a heterogeneous family of lesions with different malignant potential that arise from various trophoblast subpopulations. This study investigated the expression of p63 isoforms in various trophoblastic diseases and correlated with clinical progress, proliferation, and apoptotic activities. 4A4 and anti-p40 antibodies were applied to assess expressions of total and ΔNp63 isoforms in 20 placentas, 62 hydatidiform moles, 9 choriocarcinomas, 5 placenta site trophoblastic tumors, and 2 epithelioid trophoblastic tumors immunohistochemically. The immunoreactivity of p63 was localized to the nuclei of cytotrophoblast, villous, and chorionic-type intermediate trophoblasts with significant correlation between 2 p63 indices (P <0.001). p63 indices were significantly lower in placentas of advanced gestational age (P <0.001). Hydatidiform moles demonstrated significantly higher p63 indices than normal placentas (P <0.001). Epithelioid trophoblastic tumors displayed the highest p63 indices (45%-80%) whereas immunoreactivity was only focal in choriocarcinoma (0%-5.62%) and was essentially absent in placenta site trophoblastic tumors. There was no significant correlation between p63 indices and subsequent development of trophoblastic neoplasia in hydatidiform moles (P >0.05). Both p63 indices positively correlated with the proliferative index (Ki67) (P <0.05), apoptotic index (M30) (P <0.005), p53 (P <0.005), and p21 WAF1/CIP1 expression (P <0.005). Our results indicate that ΔNp63, the dominant isoforms expressed in trophoblasts, display heterogeneous expression patterns in relation to trophoblast subtypes. We also demonstrate for the first time the possible role of p63 in the pathogenesis of gestational trophoblastic disease (GTD) through its interaction with p53-dependent proliferation and apoptotic activities. © 2009 International Society of Gynecological Pathologists.
Persistent Identifierhttp://hdl.handle.net/10722/60382
ISSN
2015 Impact Factor: 1.437
2015 SCImago Journal Rankings: 0.723
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 7503/06M
University of Hong Kong Committee for Research and Conference
Funding Information:

Supported by research grants from the Hong Kong Research Grant Council grant (HKU 7503/06M) and the University of Hong Kong Committee for Research and Conference Grant.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorZhang, HJen_HK
dc.contributor.authorXue, WCen_HK
dc.contributor.authorSiu, MKYen_HK
dc.contributor.authorLiao, XYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-05-31T04:09:31Z-
dc.date.available2010-05-31T04:09:31Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Gynecological Pathology, 2009, v. 28 n. 2, p. 172-178en_HK
dc.identifier.issn0277-1691en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60382-
dc.description.abstractp63 regulates cell growth and differentiation and contributes to tumorigenesis through its complex isoforms. Gestational trophoblastic disease encompasses a heterogeneous family of lesions with different malignant potential that arise from various trophoblast subpopulations. This study investigated the expression of p63 isoforms in various trophoblastic diseases and correlated with clinical progress, proliferation, and apoptotic activities. 4A4 and anti-p40 antibodies were applied to assess expressions of total and ΔNp63 isoforms in 20 placentas, 62 hydatidiform moles, 9 choriocarcinomas, 5 placenta site trophoblastic tumors, and 2 epithelioid trophoblastic tumors immunohistochemically. The immunoreactivity of p63 was localized to the nuclei of cytotrophoblast, villous, and chorionic-type intermediate trophoblasts with significant correlation between 2 p63 indices (P <0.001). p63 indices were significantly lower in placentas of advanced gestational age (P <0.001). Hydatidiform moles demonstrated significantly higher p63 indices than normal placentas (P <0.001). Epithelioid trophoblastic tumors displayed the highest p63 indices (45%-80%) whereas immunoreactivity was only focal in choriocarcinoma (0%-5.62%) and was essentially absent in placenta site trophoblastic tumors. There was no significant correlation between p63 indices and subsequent development of trophoblastic neoplasia in hydatidiform moles (P >0.05). Both p63 indices positively correlated with the proliferative index (Ki67) (P <0.05), apoptotic index (M30) (P <0.005), p53 (P <0.005), and p21 WAF1/CIP1 expression (P <0.005). Our results indicate that ΔNp63, the dominant isoforms expressed in trophoblasts, display heterogeneous expression patterns in relation to trophoblast subtypes. We also demonstrate for the first time the possible role of p63 in the pathogenesis of gestational trophoblastic disease (GTD) through its interaction with p53-dependent proliferation and apoptotic activities. © 2009 International Society of Gynecological Pathologists.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.comen_HK
dc.relation.ispartofInternational Journal of Gynecological Pathologyen_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectGestational trophoblastic neoplasiaen_HK
dc.subjectP63en_HK
dc.subject.meshMembrane Proteins - biosynthesis-
dc.subject.meshPregnancy Complications - metabolism - pathology-
dc.subject.meshTrophoblastic Neoplasms - metabolism - pathology-
dc.subject.meshUterine Neoplasms - metabolism - pathology-
dc.titleP63 expression in gestational trophoblastic disease: Correlation with proliferation and apoptotic dynamicsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0277-1691&volume=28&issue=2&spage=172&epage=178&date=2009&atitle=P63+expression+in+gestational+trophoblastic+disease:+correlation+with+proliferation+and+apoptotic+dynamicsen_HK
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authoritySiu, MKY=rp00275en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/PGP.0b013e318189555ben_HK
dc.identifier.pmid19188816en_HK
dc.identifier.scopuseid_2-s2.0-68949207132en_HK
dc.identifier.hkuros161654en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68949207132&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue2en_HK
dc.identifier.spage172en_HK
dc.identifier.epage178en_HK
dc.identifier.isiWOS:000263624200012-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectAkt and p21-activated kinase signaling pathways in gestational trophoblastic disease-
dc.identifier.scopusauthoridZhang, HJ=8597830900en_HK
dc.identifier.scopusauthoridXue, WC=26640823700en_HK
dc.identifier.scopusauthoridSiu, MKY=24924018400en_HK
dc.identifier.scopusauthoridLiao, XY=7202134156en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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