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Article: Activated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices

TitleActivated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indices
Authors
KeywordsGestational trophoblastic disease
Stat3
Issue Date2008
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS
Citation
Histopathology, 2008, v. 53 n. 2, p. 139-146 How to Cite?
AbstractAims: To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters. Methods and results: By immunohistochemistry, both cytoplasmic and nuclear expression of p-Stat3-Ser 727 was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Nuclear immunoreactivity of p-Stat3-Ser 727 was significantly higher in hydatidiform mole (HM) (P < 0.001) and choriocarcinoma (P = 0.009) when compared with normal placentas. Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p-Stat3-Ser 727 expression than their normal trophoblast counterparts. Higher p-Stat3-Ser 727 expression was confirmed in choriocarcinoma cell lines, JEG-3 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p-Stat3-Ser 727 immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (P = 0.013, P = 0.039). There was a significant positive and inverse correlation between nuclear p-Stat3-Ser 727 immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (P = 0.001, P < 0.001, Spearman's ρ test) and Bcl-2 expression (P = 0.034), respectively. Conclusions: p-Stat3-Ser 727 plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. p-Stat3-Ser 727 immunoreactivity is a potential marker in predicting GTN in HM. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/60356
ISSN
2015 Impact Factor: 3.425
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative RegionHKU 7309/04M
University of Hong Kong
Funding Information:

This work was supported by grants from the Research Grants Council of the Hong Kong Special Administrative Region (HKU 7309/04M) and the Conference and Research Council grant from the University of Hong Kong.

References
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DC FieldValueLanguage
dc.contributor.authorChan, HYen_HK
dc.contributor.authorSiu, MKYen_HK
dc.contributor.authorZhang, HJen_HK
dc.contributor.authorWong, ESYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-05-31T04:09:01Z-
dc.date.available2010-05-31T04:09:01Z-
dc.date.issued2008en_HK
dc.identifier.citationHistopathology, 2008, v. 53 n. 2, p. 139-146en_HK
dc.identifier.issn0309-0167en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60356-
dc.description.abstractAims: To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters. Methods and results: By immunohistochemistry, both cytoplasmic and nuclear expression of p-Stat3-Ser 727 was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Nuclear immunoreactivity of p-Stat3-Ser 727 was significantly higher in hydatidiform mole (HM) (P < 0.001) and choriocarcinoma (P = 0.009) when compared with normal placentas. Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p-Stat3-Ser 727 expression than their normal trophoblast counterparts. Higher p-Stat3-Ser 727 expression was confirmed in choriocarcinoma cell lines, JEG-3 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p-Stat3-Ser 727 immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (P = 0.013, P = 0.039). There was a significant positive and inverse correlation between nuclear p-Stat3-Ser 727 immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (P = 0.001, P < 0.001, Spearman's ρ test) and Bcl-2 expression (P = 0.034), respectively. Conclusions: p-Stat3-Ser 727 plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. p-Stat3-Ser 727 immunoreactivity is a potential marker in predicting GTN in HM. © 2008 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HISen_HK
dc.relation.ispartofHistopathologyen_HK
dc.rightsHistopathology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectStat3en_HK
dc.subject.meshApoptosis - genetics - physiologyen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshPhosphorylationen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshSTAT3 Transcription Factor - biosynthesis - genetics - metabolismen_HK
dc.subject.meshTrophoblastic Tumor, Placental Site - genetics - metabolism - pathologyen_HK
dc.subject.meshUterine Neoplasms - genetics - metabolism - pathologyen_HK
dc.titleActivated Stat3 expression in gestational trophoblastic disease: Correlation with clinicopathological parameters and apoptotic indicesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=53&issue=2&spage=139&epage=146&date=2008&atitle=Activated+Stat3+expression+in+gestational+trophoblastic+disease:+correlation+with+clinicopathological+parameters+and+apoptotic+indicesen_HK
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authoritySiu, MKY=rp00275en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2559.2008.03089.xen_HK
dc.identifier.pmid18752497-
dc.identifier.scopuseid_2-s2.0-47749120647en_HK
dc.identifier.hkuros150697en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47749120647&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue2en_HK
dc.identifier.spage139en_HK
dc.identifier.epage146en_HK
dc.identifier.isiWOS:000258504400002-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectStem cell related genes in gestational trophoblastic diseases-
dc.identifier.scopusauthoridChan, HY=26024081600en_HK
dc.identifier.scopusauthoridSiu, MKY=24924018400en_HK
dc.identifier.scopusauthoridZhang, HJ=8597830900en_HK
dc.identifier.scopusauthoridWong, ESY=23101622300en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.citeulike3044466-

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