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Article: Gene expression profiling of human peri-implantation endometria between natural and stimulated cycles

TitleGene expression profiling of human peri-implantation endometria between natural and stimulated cycles
Authors
KeywordsEndometrium
implantation
microarray
steroid hormones
stimulated cycle
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2008, v. 90 n. 6, p. 2152-2164 How to Cite?
AbstractObjective: To investigate the effect of high serum E 2 levels in gonadotropin-stimulated cycles (hCG+7) on the gene expression patterns of human endometrium compared with natural cycles on the seventh day of LH surge (LH+7) and elucidate the underlying molecular changes that may be related to endometrial receptivity. Design: Observational study. Setting: University Hospital. Patients(s): Infertile patients with normal menstrual cycles undergoing IVF treatment. Intervention(s): Gonadotropin stimulation and endometrial biopsy. Main Outcome Measure(s): Gene expression by microarray and quantitative polymerase chain reaction (qPCR). Result(s): Endometrial samples from natural (n = 5) and stimulated (n = 8) cycles were collected. Patients in the stimulated cycles were classified as moderate (n = 4) or excessive (n = 4) responders if their serum E 2 levels on the day of administration of hCG were ≤20,000 pmol/L or >20,000 pmol/L, respectively. The RNA transcripts were profiled by Affymetrix HG-U133A microarray. Clustering and principal component analysis demonstrated a significant difference (≥2-fold) in the expression patterns of 411 genes among the three groups. Putative estrogen response elements or progesterone response elements were identified in the promoter regions of 49 differentially expressed genes of diverse biologic functions. The qPCR confirmed the microarray result in 47 endometrial samples. Conclusion(s): High serum E 2 and/or progesterone modulate the gene expression profiles of human endometrium and may affect endometrial receptivity. © 2008 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/60349
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Hong Kong Research Grant CouncilHKU 7514/05M
Funding Information:

Supported in part by grants from the Committee on Research and Conference Grant, University of Hong Kong, and the Hong Kong Research Grant Council (HKU 7514/05M).

References
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DC FieldValueLanguage
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2010-05-31T04:08:52Z-
dc.date.available2010-05-31T04:08:52Z-
dc.date.issued2008en_HK
dc.identifier.citationFertility And Sterility, 2008, v. 90 n. 6, p. 2152-2164en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60349-
dc.description.abstractObjective: To investigate the effect of high serum E 2 levels in gonadotropin-stimulated cycles (hCG+7) on the gene expression patterns of human endometrium compared with natural cycles on the seventh day of LH surge (LH+7) and elucidate the underlying molecular changes that may be related to endometrial receptivity. Design: Observational study. Setting: University Hospital. Patients(s): Infertile patients with normal menstrual cycles undergoing IVF treatment. Intervention(s): Gonadotropin stimulation and endometrial biopsy. Main Outcome Measure(s): Gene expression by microarray and quantitative polymerase chain reaction (qPCR). Result(s): Endometrial samples from natural (n = 5) and stimulated (n = 8) cycles were collected. Patients in the stimulated cycles were classified as moderate (n = 4) or excessive (n = 4) responders if their serum E 2 levels on the day of administration of hCG were ≤20,000 pmol/L or >20,000 pmol/L, respectively. The RNA transcripts were profiled by Affymetrix HG-U133A microarray. Clustering and principal component analysis demonstrated a significant difference (≥2-fold) in the expression patterns of 411 genes among the three groups. Putative estrogen response elements or progesterone response elements were identified in the promoter regions of 49 differentially expressed genes of diverse biologic functions. The qPCR confirmed the microarray result in 47 endometrial samples. Conclusion(s): High serum E 2 and/or progesterone modulate the gene expression profiles of human endometrium and may affect endometrial receptivity. © 2008 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectEndometriumen_HK
dc.subjectimplantationen_HK
dc.subjectmicroarrayen_HK
dc.subjectsteroid hormonesen_HK
dc.subjectstimulated cycleen_HK
dc.titleGene expression profiling of human peri-implantation endometria between natural and stimulated cyclesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=90&spage=2152&epage=64&date=2008&atitle=Gene+expression+profiling+of+human+peri-implantation+endometria+between+natural+and+stimulated+cycles.en_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fertnstert.2007.10.020en_HK
dc.identifier.pmid18191855-
dc.identifier.scopuseid_2-s2.0-56949085051en_HK
dc.identifier.hkuros158116en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-56949085051&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2152en_HK
dc.identifier.epage2164en_HK
dc.identifier.eissn1556-5653-
dc.identifier.isiWOS:000261566800017-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectRole of olfactomedin in implantation-
dc.identifier.scopusauthoridLiu, Y=23134878300en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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