Article: Evaluation of a rapid test for detection of H5N1 avian influenza virus

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TitleEvaluation of a rapid test for detection of H5N1 avian influenza virus
AuthorsChen, Y1
Xu, F1
Fan, X2 3
Luo, H1
Ge, S1
Zheng, Q1
Xia, N1
Chen, H2 3
Guan, Y2 3
Zhang, J1
KeywordsAvian influenza virus
Dot ELISA
H5N1
Hemagglutinin
Monoclonal antibody
Point of care
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
CitationJournal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2008.08.013
AbstractThe performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.
ISSN0166-0934
2011 Impact Factor: 2.011
2011 SCImago Journal Rankings: 0.172
DOIhttp://dx.doi.org/10.1016/j.jviromet.2008.08.013
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChen, Y
dc.contributor.authorXu, F
dc.contributor.authorFan, X
dc.contributor.authorLuo, H
dc.contributor.authorGe, S
dc.contributor.authorZheng, Q
dc.contributor.authorXia, N
dc.contributor.authorChen, H
dc.contributor.authorGuan, Y
dc.contributor.authorZhang, J
dc.date.accessioned2010-05-31T03:50:01Z
dc.date.available2010-05-31T03:50:01Z
dc.date.issued2008
dc.description.abstractThe performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.
dc.description.naturepostprint
dc.identifier.citationJournal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2008.08.013
dc.identifier.doihttp://dx.doi.org/10.1016/j.jviromet.2008.08.013
dc.identifier.epage215
dc.identifier.hkuros163957
dc.identifier.isiWOS:000261838800031
Funding AgencyGrant Number
Chinese National Project of Prevention and Treatment of Avian Influenza2004BA519A73
Chinese National Science and Technology Program2006BA1011306
Chinese National Natural and Science Foundation30640017
Science and Technology Project of Fujian Province in China2005Y020
2004YZ01-1
Xiamen Science & Technology Program3502Z20073001
Research Fund for Control of Infectious Diseases of the Hong Kong Government
Funding Information:

This work was supported by grant from Chinese National Project of Prevention and Treatment of Avian Influenza (2004BA519A73), Chinese National Science and Technology Program (2006BA1011306), Chinese National Natural and Science Foundation (30640017), Science and Technology Project of Fujian Province in China (2005Y020, 2004YZ01-1), Xiamen Science & Technology Program (3502Z20073001), and Research Fund for Control of Infectious Diseases of the Hong Kong Government.

dc.identifier.issn0166-0934
2011 Impact Factor: 2.011
2011 SCImago Journal Rankings: 0.172
dc.identifier.issue1-2
dc.identifier.openurl
dc.identifier.pmid18793671
dc.identifier.scopuseid_2-s2.0-56249097186
dc.identifier.spage213
dc.identifier.urihttp://hdl.handle.net/10722/59434
dc.identifier.volume154
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
dc.publisher.placeNetherlands
dc.relation.ispartofJournal of Virological Methods
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshAnimals
dc.subject.meshChickens
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methods
dc.subject.meshInfluenza A Virus, H5N1 Subtype - immunology - isolation and purification
dc.subject.meshInfluenza in Birds - diagnosis
dc.subjectAvian influenza virus
dc.subjectDot ELISA
dc.subjectH5N1
dc.subjectHemagglutinin
dc.subjectMonoclonal antibody
dc.subjectPoint of care
dc.titleEvaluation of a rapid test for detection of H5N1 avian influenza virus
dc.typeArticle
Author Affiliations
  1. Xiamen University
  2. The University of Hong Kong
  3. Shantou University