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Article: Evaluation of a rapid test for detection of H5N1 avian influenza virus

TitleEvaluation of a rapid test for detection of H5N1 avian influenza virus
Authors
KeywordsAvian influenza virus
Dot ELISA
H5N1
Hemagglutinin
Monoclonal antibody
Point of care
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215 How to Cite?
Abstract
The performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.
Persistent Identifierhttp://hdl.handle.net/10722/59434
ISSN
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
ISI Accession Number ID
Funding AgencyGrant Number
Chinese National Project of Prevention and Treatment of Avian Influenza2004BA519A73
Chinese National Science and Technology Program2006BA1011306
Chinese National Natural and Science Foundation30640017
Science and Technology Project of Fujian Province in China2005Y020
2004YZ01-1
Xiamen Science & Technology Program3502Z20073001
Research Fund for Control of Infectious Diseases of the Hong Kong Government
Funding Information:

This work was supported by grant from Chinese National Project of Prevention and Treatment of Avian Influenza (2004BA519A73), Chinese National Science and Technology Program (2006BA1011306), Chinese National Natural and Science Foundation (30640017), Science and Technology Project of Fujian Province in China (2005Y020, 2004YZ01-1), Xiamen Science & Technology Program (3502Z20073001), and Research Fund for Control of Infectious Diseases of the Hong Kong Government.

References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorXu, Fen_HK
dc.contributor.authorFan, Xen_HK
dc.contributor.authorLuo, Hen_HK
dc.contributor.authorGe, Sen_HK
dc.contributor.authorZheng, Qen_HK
dc.contributor.authorXia, Nen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorZhang, Jen_HK
dc.date.accessioned2010-05-31T03:50:01Z-
dc.date.available2010-05-31T03:50:01Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59434-
dc.description.abstractThe performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAvian influenza virusen_HK
dc.subjectDot ELISAen_HK
dc.subjectH5N1en_HK
dc.subjectHemagglutininen_HK
dc.subjectMonoclonal antibodyen_HK
dc.subjectPoint of careen_HK
dc.subject.meshAnimals-
dc.subject.meshChickens-
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methods-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - immunology - isolation and purification-
dc.subject.meshInfluenza in Birds - diagnosis-
dc.titleEvaluation of a rapid test for detection of H5N1 avian influenza virusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=154&issue=1-2&spage=213&epage=215&date=2008&atitle=Evaluation+of+a+rapid+test+for+detection+of+H5N1+avian+influenza+virusen_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jviromet.2008.08.013en_HK
dc.identifier.pmid18793671en_HK
dc.identifier.scopuseid_2-s2.0-56249097186en_HK
dc.identifier.hkuros163957en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-56249097186&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume154en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage213en_HK
dc.identifier.epage215en_HK
dc.identifier.isiWOS:000261838800031-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, Y=10739973700en_HK
dc.identifier.scopusauthoridXu, F=35202363800en_HK
dc.identifier.scopusauthoridFan, X=35227217200en_HK
dc.identifier.scopusauthoridLuo, H=36982678400en_HK
dc.identifier.scopusauthoridGe, S=35227303000en_HK
dc.identifier.scopusauthoridZheng, Q=35202845100en_HK
dc.identifier.scopusauthoridXia, N=35187953700en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridZhang, J=35202802400en_HK

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