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Article: Evaluation of a rapid test for detection of H5N1 avian influenza virus
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TitleEvaluation of a rapid test for detection of H5N1 avian influenza virus
 
AuthorsChen, Y1
Xu, F1
Fan, X2 3
Luo, H1
Ge, S1
Zheng, Q1
Xia, N1
Chen, H2 3
Guan, Y2 3
Zhang, J1
 
KeywordsAvian influenza virus
Dot ELISA
H5N1
Hemagglutinin
Monoclonal antibody
Point of care
 
Issue Date2008
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
CitationJournal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2008.08.013
 
AbstractThe performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.
 
ISSN0166-0934
2012 Impact Factor: 1.9
2012 SCImago Journal Rankings: 0.740
 
DOIhttp://dx.doi.org/10.1016/j.jviromet.2008.08.013
 
ISI Accession Number IDWOS:000261838800031
Funding AgencyGrant Number
Chinese National Project of Prevention and Treatment of Avian Influenza2004BA519A73
Chinese National Science and Technology Program2006BA1011306
Chinese National Natural and Science Foundation30640017
Science and Technology Project of Fujian Province in China2005Y020
2004YZ01-1
Xiamen Science & Technology Program3502Z20073001
Research Fund for Control of Infectious Diseases of the Hong Kong Government
Funding Information:

This work was supported by grant from Chinese National Project of Prevention and Treatment of Avian Influenza (2004BA519A73), Chinese National Science and Technology Program (2006BA1011306), Chinese National Natural and Science Foundation (30640017), Science and Technology Project of Fujian Province in China (2005Y020, 2004YZ01-1), Xiamen Science & Technology Program (3502Z20073001), and Research Fund for Control of Infectious Diseases of the Hong Kong Government.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChen, Y
 
dc.contributor.authorXu, F
 
dc.contributor.authorFan, X
 
dc.contributor.authorLuo, H
 
dc.contributor.authorGe, S
 
dc.contributor.authorZheng, Q
 
dc.contributor.authorXia, N
 
dc.contributor.authorChen, H
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorZhang, J
 
dc.date.accessioned2010-05-31T03:50:01Z
 
dc.date.available2010-05-31T03:50:01Z
 
dc.date.issued2008
 
dc.description.abstractThe performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was ≤0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright © 2008.
 
dc.description.naturepostprint
 
dc.identifier.citationJournal Of Virological Methods, 2008, v. 154 n. 1-2, p. 213-215 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2008.08.013
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jviromet.2008.08.013
 
dc.identifier.epage215
 
dc.identifier.hkuros163957
 
dc.identifier.isiWOS:000261838800031
Funding AgencyGrant Number
Chinese National Project of Prevention and Treatment of Avian Influenza2004BA519A73
Chinese National Science and Technology Program2006BA1011306
Chinese National Natural and Science Foundation30640017
Science and Technology Project of Fujian Province in China2005Y020
2004YZ01-1
Xiamen Science & Technology Program3502Z20073001
Research Fund for Control of Infectious Diseases of the Hong Kong Government
Funding Information:

This work was supported by grant from Chinese National Project of Prevention and Treatment of Avian Influenza (2004BA519A73), Chinese National Science and Technology Program (2006BA1011306), Chinese National Natural and Science Foundation (30640017), Science and Technology Project of Fujian Province in China (2005Y020, 2004YZ01-1), Xiamen Science & Technology Program (3502Z20073001), and Research Fund for Control of Infectious Diseases of the Hong Kong Government.

 
dc.identifier.issn0166-0934
2012 Impact Factor: 1.9
2012 SCImago Journal Rankings: 0.740
 
dc.identifier.issue1-2
 
dc.identifier.openurl
 
dc.identifier.pmid18793671
 
dc.identifier.scopuseid_2-s2.0-56249097186
 
dc.identifier.spage213
 
dc.identifier.urihttp://hdl.handle.net/10722/59434
 
dc.identifier.volume154
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Virological Methods
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAnimals
 
dc.subject.meshChickens
 
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methods
 
dc.subject.meshInfluenza A Virus, H5N1 Subtype - immunology - isolation and purification
 
dc.subject.meshInfluenza in Birds - diagnosis
 
dc.subjectAvian influenza virus
 
dc.subjectDot ELISA
 
dc.subjectH5N1
 
dc.subjectHemagglutinin
 
dc.subjectMonoclonal antibody
 
dc.subjectPoint of care
 
dc.titleEvaluation of a rapid test for detection of H5N1 avian influenza virus
 
dc.typeArticle
 
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Author Affiliations
  1. Xiamen University
  2. The University of Hong Kong
  3. Shantou University