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Conference Paper: Nano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental water

TitleNano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental water
Authors
KeywordsAg
Biosensor
E. coli
PCR
Piezoelectric quartz crystal
TiO2
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios
Citation
The 10th World Congress on Biosensors Shangai, China, 14-16 May 2008. In Biosensors And Bioelectronics, 2009, v. 24 n. 5, p. 1405-1410 How to Cite?
AbstractTo meet the requirement of World Health Organization for zero tolerance of E. coli cell in 100 mL drinking water, a new procedure based on photodeposition of nano-Ag at TiO2-coated piezoelectric quartz crystal (PQC) electrode was developed to fabricate a highly sensitive PQC/DNA biosensor. Enhancement of 3.3 times for binding of complementary DNA has been shown and attributed to the following effects arising from the nano-Ag coating. First, a large increase in the active surface area and packing density of neutravidin enhances the maximum neutravidin load to 1.8 times of a normal electrode. Second, the functional activity of neutravidin is enhanced by chemical interaction with nano-Ag to give rise to an increase in the binding ratio between neutravidin and biotinylated DNA probe from 1.00:1.76 to 1.00:3.01. Third, the stronger binding leads to a higher stability of the biotinylated DNA probes bound and increase in hybridization with the complementary DNA. Under the optimized conditions for flow analysis with online PCR product denaturing and hybridization, a detection limit of eight E. coli cells are obtained which require sampling at least 800 mL water to detect a single E. coli cell in 100 mL water. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58408
ISSN
2015 Impact Factor: 7.476
2015 SCImago Journal Rankings: 2.150
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Hen_HK
dc.contributor.authorChoy, TSen_HK
dc.contributor.authorZhu, DRen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2010-05-31T03:29:46Z-
dc.date.available2010-05-31T03:29:46Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 10th World Congress on Biosensors Shangai, China, 14-16 May 2008. In Biosensors And Bioelectronics, 2009, v. 24 n. 5, p. 1405-1410en_HK
dc.identifier.issn0956-5663en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58408-
dc.description.abstractTo meet the requirement of World Health Organization for zero tolerance of E. coli cell in 100 mL drinking water, a new procedure based on photodeposition of nano-Ag at TiO2-coated piezoelectric quartz crystal (PQC) electrode was developed to fabricate a highly sensitive PQC/DNA biosensor. Enhancement of 3.3 times for binding of complementary DNA has been shown and attributed to the following effects arising from the nano-Ag coating. First, a large increase in the active surface area and packing density of neutravidin enhances the maximum neutravidin load to 1.8 times of a normal electrode. Second, the functional activity of neutravidin is enhanced by chemical interaction with nano-Ag to give rise to an increase in the binding ratio between neutravidin and biotinylated DNA probe from 1.00:1.76 to 1.00:3.01. Third, the stronger binding leads to a higher stability of the biotinylated DNA probes bound and increase in hybridization with the complementary DNA. Under the optimized conditions for flow analysis with online PCR product denaturing and hybridization, a detection limit of eight E. coli cells are obtained which require sampling at least 800 mL water to detect a single E. coli cell in 100 mL water. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biosen_HK
dc.relation.ispartofBiosensors and Bioelectronicsen_HK
dc.subjectAgen_HK
dc.subjectBiosensoren_HK
dc.subjectE. colien_HK
dc.subjectPCRen_HK
dc.subjectPiezoelectric quartz crystalen_HK
dc.subjectTiO2en_HK
dc.subject.meshBiosensing Techniques - instrumentation - methods-
dc.subject.meshDNA, Bacterial - isolation and purification-
dc.subject.meshEnvironmental Monitoring - instrumentation-
dc.subject.meshEscherichia coli - genetics - isolation and purification-
dc.subject.meshMicro-Electrical-Mechanical Systems - instrumentation-
dc.titleNano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental wateren_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0956-5663&volume=24&issue=5&spage=1405&epage=1410&date=2009&atitle=Nano-silver-modified+PQC/DNA+biosensor+for+detecting+E.+coli+in+environmental+wateren_HK
dc.identifier.emailSun, H:sunhui@hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authoritySun, H=rp00778en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bios.2008.08.008en_HK
dc.identifier.pmid19022649-
dc.identifier.scopuseid_2-s2.0-58149154662en_HK
dc.identifier.hkuros164229en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149154662&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1405en_HK
dc.identifier.epage1410en_HK
dc.identifier.eissn1873-4235-
dc.identifier.isiWOS:000263199800058-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridSun, H=37032038600en_HK
dc.identifier.scopusauthoridChoy, TS=35112985400en_HK
dc.identifier.scopusauthoridZhu, DR=7403599128en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK
dc.customcontrol.immutablesml 160614 amended-

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