File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Over-expression of FOXM1 transcription factor is associated with cervical cancer progression and pathogenesis
  • Basic View
  • Metadata View
  • XML View
TitleOver-expression of FOXM1 transcription factor is associated with cervical cancer progression and pathogenesis
 
AuthorsChan, DW1
Yu, SYM1
Chiu, PM1
Yao, KM1
Liu, VWS1
Cheung, ANY1
Ngan, HYS1 2
 
KeywordsCell proliferation
Cervical cancer
FOXM1
Immunohistochemistry
 
Issue Date2008
 
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
 
CitationJournal Of Pathology, 2008, v. 215 n. 3, p. 245-252 [How to Cite?]
DOI: http://dx.doi.org/10.1002/path.2355
 
AbstractThe Forkhead Box M1 (FOXM1) transcription factor plays a crucial role in regulating expression of cell cycle genes which are essentially involved in cell proliferation, differentiation and transformation. Recent studies have reported that aberrant expression of FOXM1 in a variety of human cancers is associated with their aggressive behaviour. However, the functional significance of FOXM1 in human cervical cancer is not known. We have shown that FOXM1 was significantly over-expressed in cervical squamous cell carcinoma (SCC) compared to normal cervical epithelium immunohistochemically (p < 0.001). In addition, intratumoural FOXM1 positivity was increased in cervical intraepithelial neoplasia (CIN) and carcinoma, compared with that in normal epithelium, indicating that FOXM1 is involved in tumour progression. Indeed, this is supported by clinicopathological analysis that the overexpression of FOXM1 was significantly associated with tumour late stage (p = 0.012) and cell proliferation marker, Ki67 (p < 0.001). Functionally, enforced expression of FOXM1c in FOXM1-deficient cervical cancer cells (C33A) remarkably enhanced cell proliferation and anchorage-independent growth ability. Conversely, depletion of FOXM1 by RNA interference in FOXM1-over-expressing cervical cancer cells (SiHa) caused significant inhibition on cell proliferation and anchorage-independent growth ability on soft agar. This inhibitory phenomenon was associated with the reduced expressions of cyclin B1, cyclinD1 and cdc25B but increased expression of p27 Kip1 and p21 Cip1. Our findings suggest a role for FOXM1 in the development and pathogenesis of human cervical SCC. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
 
ISSN0022-3417
2013 Impact Factor: 7.330
 
DOIhttp://dx.doi.org/10.1002/path.2355
 
ISI Accession Number IDWOS:000257332500005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, DW
 
dc.contributor.authorYu, SYM
 
dc.contributor.authorChiu, PM
 
dc.contributor.authorYao, KM
 
dc.contributor.authorLiu, VWS
 
dc.contributor.authorCheung, ANY
 
dc.contributor.authorNgan, HYS
 
dc.date.accessioned2010-05-31T03:27:07Z
 
dc.date.available2010-05-31T03:27:07Z
 
dc.date.issued2008
 
dc.description.abstractThe Forkhead Box M1 (FOXM1) transcription factor plays a crucial role in regulating expression of cell cycle genes which are essentially involved in cell proliferation, differentiation and transformation. Recent studies have reported that aberrant expression of FOXM1 in a variety of human cancers is associated with their aggressive behaviour. However, the functional significance of FOXM1 in human cervical cancer is not known. We have shown that FOXM1 was significantly over-expressed in cervical squamous cell carcinoma (SCC) compared to normal cervical epithelium immunohistochemically (p < 0.001). In addition, intratumoural FOXM1 positivity was increased in cervical intraepithelial neoplasia (CIN) and carcinoma, compared with that in normal epithelium, indicating that FOXM1 is involved in tumour progression. Indeed, this is supported by clinicopathological analysis that the overexpression of FOXM1 was significantly associated with tumour late stage (p = 0.012) and cell proliferation marker, Ki67 (p < 0.001). Functionally, enforced expression of FOXM1c in FOXM1-deficient cervical cancer cells (C33A) remarkably enhanced cell proliferation and anchorage-independent growth ability. Conversely, depletion of FOXM1 by RNA interference in FOXM1-over-expressing cervical cancer cells (SiHa) caused significant inhibition on cell proliferation and anchorage-independent growth ability on soft agar. This inhibitory phenomenon was associated with the reduced expressions of cyclin B1, cyclinD1 and cdc25B but increased expression of p27 Kip1 and p21 Cip1. Our findings suggest a role for FOXM1 in the development and pathogenesis of human cervical SCC. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Pathology, 2008, v. 215 n. 3, p. 245-252 [How to Cite?]
DOI: http://dx.doi.org/10.1002/path.2355
 
dc.identifier.doihttp://dx.doi.org/10.1002/path.2355
 
dc.identifier.eissn1096-9896
 
dc.identifier.epage252
 
dc.identifier.hkuros144697
 
dc.identifier.isiWOS:000257332500005
 
dc.identifier.issn0022-3417
2013 Impact Factor: 7.330
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid18464245
 
dc.identifier.scopuseid_2-s2.0-46849097424
 
dc.identifier.spage245
 
dc.identifier.urihttp://hdl.handle.net/10722/58268
 
dc.identifier.volume215
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Pathology. Copyright © John Wiley & Sons Ltd.
 
dc.subject.meshBiological Markers - analysis
 
dc.subject.meshBlotting, Western - methods
 
dc.subject.meshCarcinoma, Squamous Cell - genetics
 
dc.subject.meshCell Cycle Proteins - analysis - genetics
 
dc.subject.meshCell Proliferation
 
dc.subject.meshCervix Uteri - chemistry - metabolism
 
dc.subject.meshFemale
 
dc.subject.meshForkhead Transcription Factors - analysis - genetics
 
dc.subject.meshGene Expression
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeoplasm Staging
 
dc.subject.meshStatistics, Nonparametric
 
dc.subject.meshTumor Markers, Biological - analysis
 
dc.subject.meshUterine Cervical Neoplasms - genetics
 
dc.subjectCell proliferation
 
dc.subjectCervical cancer
 
dc.subjectFOXM1
 
dc.subjectImmunohistochemistry
 
dc.titleOver-expression of FOXM1 transcription factor is associated with cervical cancer progression and pathogenesis
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Chan, DW</contributor.author>
<contributor.author>Yu, SYM</contributor.author>
<contributor.author>Chiu, PM</contributor.author>
<contributor.author>Yao, KM</contributor.author>
<contributor.author>Liu, VWS</contributor.author>
<contributor.author>Cheung, ANY</contributor.author>
<contributor.author>Ngan, HYS</contributor.author>
<date.accessioned>2010-05-31T03:27:07Z</date.accessioned>
<date.available>2010-05-31T03:27:07Z</date.available>
<date.issued>2008</date.issued>
<identifier.citation>Journal Of Pathology, 2008, v. 215 n. 3, p. 245-252</identifier.citation>
<identifier.issn>0022-3417</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/58268</identifier.uri>
<description.abstract>The Forkhead Box M1 (FOXM1) transcription factor plays a crucial role in regulating expression of cell cycle genes which are essentially involved in cell proliferation, differentiation and transformation. Recent studies have reported that aberrant expression of FOXM1 in a variety of human cancers is associated with their aggressive behaviour. However, the functional significance of FOXM1 in human cervical cancer is not known. We have shown that FOXM1 was significantly over-expressed in cervical squamous cell carcinoma (SCC) compared to normal cervical epithelium immunohistochemically (p &lt; 0.001). In addition, intratumoural FOXM1 positivity was increased in cervical intraepithelial neoplasia (CIN) and carcinoma, compared with that in normal epithelium, indicating that FOXM1 is involved in tumour progression. Indeed, this is supported by clinicopathological analysis that the overexpression of FOXM1 was significantly associated with tumour late stage (p = 0.012) and cell proliferation marker, Ki67 (p &lt; 0.001). Functionally, enforced expression of FOXM1c in FOXM1-deficient cervical cancer cells (C33A) remarkably enhanced cell proliferation and anchorage-independent growth ability. Conversely, depletion of FOXM1 by RNA interference in FOXM1-over-expressing cervical cancer cells (SiHa) caused significant inhibition on cell proliferation and anchorage-independent growth ability on soft agar. This inhibitory phenomenon was associated with the reduced expressions of cyclin B1, cyclinD1 and cdc25B but increased expression of p27 Kip1 and p21 Cip1. Our findings suggest a role for FOXM1 in the development and pathogenesis of human cervical SCC. Copyright &#169; 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.</description.abstract>
<language>eng</language>
<publisher>John Wiley &amp; Sons. The Journal&apos;s web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130</publisher>
<relation.ispartof>Journal of Pathology</relation.ispartof>
<rights>Journal of Pathology. Copyright &#169; John Wiley &amp; Sons Ltd.</rights>
<subject>Cell proliferation</subject>
<subject>Cervical cancer</subject>
<subject>FOXM1</subject>
<subject>Immunohistochemistry</subject>
<subject.mesh>Biological Markers - analysis</subject.mesh>
<subject.mesh>Blotting, Western - methods</subject.mesh>
<subject.mesh>Carcinoma, Squamous Cell - genetics</subject.mesh>
<subject.mesh>Cell Cycle Proteins - analysis - genetics</subject.mesh>
<subject.mesh>Cell Proliferation</subject.mesh>
<subject.mesh>Cervix Uteri - chemistry - metabolism</subject.mesh>
<subject.mesh>Female</subject.mesh>
<subject.mesh>Forkhead Transcription Factors - analysis - genetics</subject.mesh>
<subject.mesh>Gene Expression</subject.mesh>
<subject.mesh>Gene Expression Regulation, Neoplastic</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Immunohistochemistry</subject.mesh>
<subject.mesh>Middle Aged</subject.mesh>
<subject.mesh>Neoplasm Staging</subject.mesh>
<subject.mesh>Statistics, Nonparametric</subject.mesh>
<subject.mesh>Tumor Markers, Biological - analysis</subject.mesh>
<subject.mesh>Uterine Cervical Neoplasms - genetics</subject.mesh>
<title>Over-expression of FOXM1 transcription factor is associated with cervical cancer progression and pathogenesis</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0022-3417&amp;volume=215 &amp;issue=3&amp;spage=245&amp;epage=252&amp;date=2008&amp;atitle=Over-expression+of+FOXM1+transcription+factor+is+associated+with+cervical+cancer+progression+and+pathogenesis</identifier.openurl>
<description.nature>link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1002/path.2355</identifier.doi>
<identifier.pmid>18464245</identifier.pmid>
<identifier.scopus>eid_2-s2.0-46849097424</identifier.scopus>
<identifier.hkuros>144697</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-46849097424&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>215</identifier.volume>
<identifier.issue>3</identifier.issue>
<identifier.spage>245</identifier.spage>
<identifier.epage>252</identifier.epage>
<identifier.eissn>1096-9896</identifier.eissn>
<identifier.isi>WOS:000257332500005</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Queen Mary Hospital Hong Kong