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Article: Serological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infection

TitleSerological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infection
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0163-2116
Citation
Digestive Diseases And Sciences, 2008, v. 53 n. 12, p. 3112-3118 How to Cite?
AbstractProteomic technology was employed to analyze serum samples from healthy subjects (10 cases) and gastritis patients with negative and positive Helicobacter pylori (Hp) infection (15 cases each). The serum proteins were separated by two-dimensional (2-D) gel electrophoresis and analyzed by a computer-aided program. The altered proteins in expression were then identified by mass spectrometry and validated by Western blotting. Compared to those in normal control, proteins in at least six areas of 2-D gels were found to significantly increase their expression levels in both Hp-negative and Hp-positive serum samples. These proteins were identified by mass peptide fingerprinting and confirmed by Western blotting to be the truncated or cleaved protein fragments of apolipoprotein A-I and alpha-1 antitrypsin, two well-known acute-phase proteins. We conclude that the degradation or metabolization of acute-phase proteins, apolipoprotein A-I, and alpha1-antitrypsin, is a common response to gastric inflammation irrespective of Hp infection. © 2008 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/58229
ISSN
2015 Impact Factor: 2.516
2015 SCImago Journal Rankings: 0.995
ISI Accession Number ID
Funding AgencyGrant Number
Guangzhou Municipal Scheme of Science and Technology2007Z3-E4021
Chang-Jiang Scholars Program 2007
Hong Kong Research Grants CouncilHKU 7218/02M
Funding Information:

This work was partially supported by Guangzhou Municipal Scheme of Science and Technology 2007Z3-E4021 and Chang-Jiang Scholars Program 2007 (to Q. Y. H.) and Hong Kong Research Grants Council Grants HKU 7218/02M (to J. F. C.).

References

 

DC FieldValueLanguage
dc.contributor.authorHe, QYen_HK
dc.contributor.authorYang, Hen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorChiu, JFen_HK
dc.date.accessioned2010-05-31T03:26:14Z-
dc.date.available2010-05-31T03:26:14Z-
dc.date.issued2008en_HK
dc.identifier.citationDigestive Diseases And Sciences, 2008, v. 53 n. 12, p. 3112-3118en_HK
dc.identifier.issn0163-2116en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58229-
dc.description.abstractProteomic technology was employed to analyze serum samples from healthy subjects (10 cases) and gastritis patients with negative and positive Helicobacter pylori (Hp) infection (15 cases each). The serum proteins were separated by two-dimensional (2-D) gel electrophoresis and analyzed by a computer-aided program. The altered proteins in expression were then identified by mass spectrometry and validated by Western blotting. Compared to those in normal control, proteins in at least six areas of 2-D gels were found to significantly increase their expression levels in both Hp-negative and Hp-positive serum samples. These proteins were identified by mass peptide fingerprinting and confirmed by Western blotting to be the truncated or cleaved protein fragments of apolipoprotein A-I and alpha-1 antitrypsin, two well-known acute-phase proteins. We conclude that the degradation or metabolization of acute-phase proteins, apolipoprotein A-I, and alpha1-antitrypsin, is a common response to gastric inflammation irrespective of Hp infection. © 2008 Springer Science+Business Media, LLC.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0163-2116en_HK
dc.relation.ispartofDigestive Diseases and Sciencesen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshApolipoprotein A-I - metabolismen_HK
dc.subject.meshBiological Markers - metabolismen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGastritis - metabolism - microbiologyen_HK
dc.subject.meshHelicobacter Infections - metabolismen_HK
dc.subject.meshHelicobacter pylori - pathogenicityen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInflammation - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeptide Fragments - metabolismen_HK
dc.subject.meshProteomicsen_HK
dc.subject.meshStomach - microbiologyen_HK
dc.subject.meshalpha 1-Antitrypsin - metabolismen_HK
dc.titleSerological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infectionen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10620-008-0269-zen_HK
dc.identifier.pmid18594986-
dc.identifier.scopuseid_2-s2.0-55949091399en_HK
dc.identifier.hkuros150114en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55949091399&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3112en_HK
dc.identifier.epage3118en_HK
dc.identifier.isiWOS:000260837000009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK
dc.identifier.scopusauthoridYang, H=14036443600en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK

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