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- Publisher Website: 10.1007/s10620-008-0269-z
- Scopus: eid_2-s2.0-55949091399
- PMID: 18594986
- WOS: WOS:000260837000009
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Article: Serological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infection
Title | Serological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infection | ||||||||
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Authors | |||||||||
Keywords | Acute-phase proteins Biomarkers Gastritis Helicobacter pylori infection Proteomics Serological proteins | ||||||||
Issue Date | 2008 | ||||||||
Publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0163-2116 | ||||||||
Citation | Digestive Diseases And Sciences, 2008, v. 53 n. 12, p. 3112-3118 How to Cite? | ||||||||
Abstract | Proteomic technology was employed to analyze serum samples from healthy subjects (10 cases) and gastritis patients with negative and positive Helicobacter pylori (Hp) infection (15 cases each). The serum proteins were separated by two-dimensional (2-D) gel electrophoresis and analyzed by a computer-aided program. The altered proteins in expression were then identified by mass spectrometry and validated by Western blotting. Compared to those in normal control, proteins in at least six areas of 2-D gels were found to significantly increase their expression levels in both Hp-negative and Hp-positive serum samples. These proteins were identified by mass peptide fingerprinting and confirmed by Western blotting to be the truncated or cleaved protein fragments of apolipoprotein A-I and alpha-1 antitrypsin, two well-known acute-phase proteins. We conclude that the degradation or metabolization of acute-phase proteins, apolipoprotein A-I, and alpha1-antitrypsin, is a common response to gastric inflammation irrespective of Hp infection. © 2008 Springer Science+Business Media, LLC. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/58229 | ||||||||
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 1.068 | ||||||||
ISI Accession Number ID |
Funding Information: This work was partially supported by Guangzhou Municipal Scheme of Science and Technology 2007Z3-E4021 and Chang-Jiang Scholars Program 2007 (to Q. Y. H.) and Hong Kong Research Grants Council Grants HKU 7218/02M (to J. F. C.). | ||||||||
References | |||||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | He, QY | en_HK |
dc.contributor.author | Yang, H | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.contributor.author | Chiu, JF | en_HK |
dc.date.accessioned | 2010-05-31T03:26:14Z | - |
dc.date.available | 2010-05-31T03:26:14Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Digestive Diseases And Sciences, 2008, v. 53 n. 12, p. 3112-3118 | en_HK |
dc.identifier.issn | 0163-2116 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/58229 | - |
dc.description.abstract | Proteomic technology was employed to analyze serum samples from healthy subjects (10 cases) and gastritis patients with negative and positive Helicobacter pylori (Hp) infection (15 cases each). The serum proteins were separated by two-dimensional (2-D) gel electrophoresis and analyzed by a computer-aided program. The altered proteins in expression were then identified by mass spectrometry and validated by Western blotting. Compared to those in normal control, proteins in at least six areas of 2-D gels were found to significantly increase their expression levels in both Hp-negative and Hp-positive serum samples. These proteins were identified by mass peptide fingerprinting and confirmed by Western blotting to be the truncated or cleaved protein fragments of apolipoprotein A-I and alpha-1 antitrypsin, two well-known acute-phase proteins. We conclude that the degradation or metabolization of acute-phase proteins, apolipoprotein A-I, and alpha1-antitrypsin, is a common response to gastric inflammation irrespective of Hp infection. © 2008 Springer Science+Business Media, LLC. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0163-2116 | en_HK |
dc.relation.ispartof | Digestive Diseases and Sciences | en_HK |
dc.subject | Acute-phase proteins | - |
dc.subject | Biomarkers | - |
dc.subject | Gastritis | - |
dc.subject | Helicobacter pylori infection | - |
dc.subject | Proteomics | - |
dc.subject | Serological proteins | - |
dc.subject.mesh | Adult | en_HK |
dc.subject.mesh | Aged | en_HK |
dc.subject.mesh | Aged, 80 and over | en_HK |
dc.subject.mesh | Apolipoprotein A-I - metabolism | en_HK |
dc.subject.mesh | Biological Markers - metabolism | en_HK |
dc.subject.mesh | Case-Control Studies | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Gastritis - metabolism - microbiology | en_HK |
dc.subject.mesh | Helicobacter Infections - metabolism | en_HK |
dc.subject.mesh | Helicobacter pylori - pathogenicity | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Inflammation - metabolism | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Middle Aged | en_HK |
dc.subject.mesh | Peptide Fragments - metabolism | en_HK |
dc.subject.mesh | Proteomics | en_HK |
dc.subject.mesh | Stomach - microbiology | en_HK |
dc.subject.mesh | alpha 1-Antitrypsin - metabolism | en_HK |
dc.title | Serological proteomics of gastritis: Degradation of apolipoprotein A-I and alpha1-antitrypsin is a common response to inflammation irrespective of Helicobacter pylori infection | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wong, BCY:bcywong@hku.hk | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s10620-008-0269-z | en_HK |
dc.identifier.pmid | 18594986 | - |
dc.identifier.scopus | eid_2-s2.0-55949091399 | en_HK |
dc.identifier.hkuros | 150114 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-55949091399&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 53 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 3112 | en_HK |
dc.identifier.epage | 3118 | en_HK |
dc.identifier.isi | WOS:000260837000009 | - |
dc.publisher.place | United States | en_HK |
dc.relation.project | Regulation of [alpha]-fetoprotein gene expression in differentiating and cancer cells | - |
dc.identifier.scopusauthorid | He, QY=34770287900 | en_HK |
dc.identifier.scopusauthorid | Yang, H=14036443600 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.scopusauthorid | Chiu, JF=7201501692 | en_HK |
dc.identifier.issnl | 0163-2116 | - |