Regulation of [alpha]-fetoprotein gene expression in differentiating and cancer cells
Dr Chiu, Jen-fu (Principal investigator)
genes, fetoprotein, cancer
RGC General Research Fund (GRF)
HKU Project Code
General Research Fund (GRF)
The project attempts to: (1) Identify and characterize DAS-binding protein (DAP) that regulate [alpha]-fetoprotein expression; (2) Investigate the specific DNA binding activity and biological function of DAP. The biological function will be determined by using various mutant genes, DAP antisense sequence and transcription factor decoys; (3) Identify other genes that are regulated by the DAS cis-element through a computer-assisted analysis of the genomic sequences in GenBank; and (4) Investigate the expression of these candidate genes in F9 cells during differentiation, and in developing liver cells and hepatomas.