Regulation of [alpha]-fetoprotein gene expression in differentiating and cancer cells


Grant Data
Project Title
Regulation of [alpha]-fetoprotein gene expression in differentiating and cancer cells
Principal Investigator
Dr Chiu, Jen-fu   (Principal investigator)
Duration
52
Start Date
2002-09-01
Completion Date
2006-12-31
Amount
2018512
Conference Title
Presentation Title
Keywords
genes, fetoprotein, cancer
Discipline
Genetic Disease
Sponsor
RGC General Research Fund (GRF)
HKU Project Code
HKU 7218/02M
Grant Type
General Research Fund (GRF)
Funding Year
2002/2003
Status
Completed
Objectives
The project attempts to: (1) Identify and characterize DAS-binding protein (DAP) that regulate [alpha]-fetoprotein expression; (2) Investigate the specific DNA binding activity and biological function of DAP. The biological function will be determined by using various mutant genes, DAP antisense sequence and transcription factor decoys; (3) Identify other genes that are regulated by the DAS cis-element through a computer-assisted analysis of the genomic sequences in GenBank; and (4) Investigate the expression of these candidate genes in F9 cells during differentiation, and in developing liver cells and hepatomas.