Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hepatoprotective effects of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced acute liver hepatotoxicity in rats

TitleHepatoprotective effects of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced acute liver hepatotoxicity in rats
Authors
KeywordsAlanine aminotransferase
Aspartate aminotransferase
Carbon tetrachloride
Coptidis rhizoma aqueous extract
Liver histopathology
Superoxide dismutase
Issue Date2009
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 130-136 How to Cite?
AbstractAim of the study: Coptidis rhizoma (CR, Chinese name is Huanglian) has been used in treating infectious and inflammatory diseases for two thousand years in Traditional Chinese Medicine (TCM). Its related pharmacological basis for the therapeutics has been studied intensively, but CR can also be used for vomiting of "dampness-heat type or acid regurgitation" due to "liver-fire attacking stomach" in TCM, whose symptoms seem to link the hepatic and biliary disorders, yet details in the therapies of liver diseases and underlying mechanism(s) remain unclear. To clarify this ethnopharmacological relevance, hepatoprotective effect of Coptidis rhizoma aqueous extract (CRAE) and its possible mechanism were studied in rats intoxicated with carbon tetrachloride (CCl 4) in the present study. Materials and methods: Sprague-Dawley (SD) rats aged 7 weeks old were intraperitoneally injected with CCl 4 at a dose of 1.0 ml/kg as a 50% olive oil solution. The rats were orally given the CRAE at doses of 400, 600, 800 mg/kg and 120 mg/kg berberine body weight (BW) after 6 h of CCl 4 treatment. At 24 h after CCl 4 injection, samples of blood and liver were collected and then biochemical parameters and histological studies were carried out. Results: The results showed that CRAE and berberine inhibited significantly the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and increased the activity of superoxide dismutase (SOD). Observation on the hepatoprotective effect of berberine was consistent to that of CRAE. Conclusion: The study is the first time to demonstrate that CRAE has hepatoprotective effect on acute liver injuries induced by CCl 4, and the results suggest that the effect of CRAE against CCl 4-induced liver damage is related to antioxidant property. © 2009 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58180
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong10206540
10208005
21362502
Medical Faculty Research GrantPong Ding Yueng Endowment Fund for Education Research20005274
University Grant Committee (UGC) of Hong Kong764708 M
AoE/P-10/01
Funding Information:

The study was financially supported by grants from the research council of the University of Hong Kong (Project Codes: 10206540, 10208005), Medical Faculty Research Grant, the University of Hong Kong (Project Code: 21362502), Pong Ding Yueng Endowment Fund for Education & Research (Project Code: 20005274), The University Grant Committee (UGC) of Hong Kong (Project Code: 764708 M) and The University Grant Committee of Hong Kong (Area of Excellence Scheme, AoE/P-10/01). The authors are grateful to the support of Professors Yung-Chi Cheng, Chi-Ming Che and Allan SY Lau. The authors would also like to express special thanks to Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorYe, Xen_HK
dc.contributor.authorFeng, Yen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorTsao, Sen_HK
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorSze, Cen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorTang, Jen_HK
dc.contributor.authorShen, Jen_HK
dc.contributor.authorKobayashi, Sen_HK
dc.date.accessioned2010-05-31T03:25:18Z-
dc.date.available2010-05-31T03:25:18Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 130-136en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58180-
dc.description.abstractAim of the study: Coptidis rhizoma (CR, Chinese name is Huanglian) has been used in treating infectious and inflammatory diseases for two thousand years in Traditional Chinese Medicine (TCM). Its related pharmacological basis for the therapeutics has been studied intensively, but CR can also be used for vomiting of "dampness-heat type or acid regurgitation" due to "liver-fire attacking stomach" in TCM, whose symptoms seem to link the hepatic and biliary disorders, yet details in the therapies of liver diseases and underlying mechanism(s) remain unclear. To clarify this ethnopharmacological relevance, hepatoprotective effect of Coptidis rhizoma aqueous extract (CRAE) and its possible mechanism were studied in rats intoxicated with carbon tetrachloride (CCl 4) in the present study. Materials and methods: Sprague-Dawley (SD) rats aged 7 weeks old were intraperitoneally injected with CCl 4 at a dose of 1.0 ml/kg as a 50% olive oil solution. The rats were orally given the CRAE at doses of 400, 600, 800 mg/kg and 120 mg/kg berberine body weight (BW) after 6 h of CCl 4 treatment. At 24 h after CCl 4 injection, samples of blood and liver were collected and then biochemical parameters and histological studies were carried out. Results: The results showed that CRAE and berberine inhibited significantly the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and increased the activity of superoxide dismutase (SOD). Observation on the hepatoprotective effect of berberine was consistent to that of CRAE. Conclusion: The study is the first time to demonstrate that CRAE has hepatoprotective effect on acute liver injuries induced by CCl 4, and the results suggest that the effect of CRAE against CCl 4-induced liver damage is related to antioxidant property. © 2009 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAlanine aminotransferaseen_HK
dc.subjectAspartate aminotransferaseen_HK
dc.subjectCarbon tetrachlorideen_HK
dc.subjectCoptidis rhizoma aqueous extracten_HK
dc.subjectLiver histopathologyen_HK
dc.subjectSuperoxide dismutaseen_HK
dc.subject.meshBerberine - pharmacology - therapeutic use-
dc.subject.meshCoptis - chemistry-
dc.subject.meshDrug-Induced Liver Injury - prevention and control-
dc.subject.meshDrugs, Chinese Herbal - pharmacology - therapeutic use-
dc.subject.meshLiver - drug effects - pathology-
dc.titleHepatoprotective effects of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced acute liver hepatotoxicity in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=124&issue=1&spage=130&epage=136&date=2009&atitle=Hepatoprotective+effects+of+Coptidis+rhizoma+aqueous+extract+on+carbon+tetrachloride-induced+acute+liver+hepatotoxicity+in+ratsen_HK
dc.identifier.emailFeng, Y: yfeng@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailNg, KM: kwanmng@hku.hken_HK
dc.identifier.emailTsao, S: gswtsao@hku.hken_HK
dc.identifier.emailSze, C: stephens@hku.hken_HK
dc.identifier.emailZhang, Y: ybzhang@hku.hken_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authorityFeng, Y=rp00466en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityTsao, S=rp00399en_HK
dc.identifier.authoritySze, C=rp00514en_HK
dc.identifier.authorityZhang, Y=rp01410en_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jep.2009.04.003en_HK
dc.identifier.pmid19536921-
dc.identifier.scopuseid_2-s2.0-67349130113en_HK
dc.identifier.hkuros155596en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349130113&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume124en_HK
dc.identifier.issue1en_HK
dc.identifier.spage130en_HK
dc.identifier.epage136en_HK
dc.identifier.eissn1872-7573-
dc.identifier.isiWOS:000268201200016-
dc.publisher.placeIrelanden_HK
dc.relation.projectInstitute of molecular technology for drug discovery and synthesis-
dc.identifier.scopusauthoridYe, X=35277763000en_HK
dc.identifier.scopusauthoridFeng, Y=24467969600en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridNg, KM=26026091100en_HK
dc.identifier.scopusauthoridTsao, S=7102813116en_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK
dc.identifier.scopusauthoridSze, C=23482617000en_HK
dc.identifier.scopusauthoridZhang, Y=23483121900en_HK
dc.identifier.scopusauthoridTang, J=37023546200en_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.scopusauthoridKobayashi, S=8083633200en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats