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Article: Involvement of cystic fibrosis transmembrane conductance regulator (CFTR) in the pathogenesis of hydrosalpinx induced by Chlamydia trachomatis infection

TitleInvolvement of cystic fibrosis transmembrane conductance regulator (CFTR) in the pathogenesis of hydrosalpinx induced by Chlamydia trachomatis infection
Authors
KeywordsCFTR
Chlamydia trachomatis
Hydrosalpinx fluid
Infection
Infertility
Issue Date2008
PublisherWiley-Blackwell Publishing Asia.
Citation
Journal Of Obstetrics And Gynaecology Research, 2008, v. 34 n. 6, p. 923-930 How to Cite?
AbstractBackground: Genital Chlamydia (C) trachomatis infection has been recognized as the single most common cause of pelvic inflammatory disease leading to severe tubal damage, ectopic pregnancy, infertility and hydrosalpinx. However, the mechanism underlying the formation of hydrosalpinx induced by C. trachomatis infection remains largely unknown. We performed this study to determine the involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel that regulates epithelial electrolyte and fluid secretion, in hydrosalpinx fluid formation. Methods: Western blot analysis was used to determine CFTR expression in the hydrosalpinges that were seen on the ultrasound scans of infertile assisted reproduction treatment patients. Correlation with C. trachomatis infection was done by testing patients' sera for C. trachomatis immunoglobulin G antibody titer using a Capita enzyme-linked immunosorbent assay based kit. CFTR involvement was further verified in a rat C. trachomatis infection model and confirmed using CFTR mutant (CFTR tm1Unc) mice. Results: Here we report on the up-regulated expression of CFTR in the hydrosalpinx tissues of infertile patients with detectable serum levels of C. trachomatis antibody (immunoglobulin G). In a rat model, increased CFTR expression and fluid accumulation could be observed in the uterine horns infected with C. trachomatis elementary bodies, which was reversed by antibiotics treatment. In C. trachomatis-infected CFTR tm1Unc mice, however, no detectable fluid accumulation was observed. Conclusion: These findings suggest the involvement of CFTR in the pathogenesis of hydrosalpinx fluid formation and may provide grounds for a better treatment strategy to improve assisted reproduction treatment outcome in infertile patients with hydrosalpinx. © 2008 Japan Society of Obstetrics and Gynecology.
Persistent Identifierhttp://hdl.handle.net/10722/58062
ISSN
2015 Impact Factor: 1.091
2015 SCImago Journal Rankings: 0.521
ISI Accession Number ID
Funding AgencyGrant Number
LKS Institute of Health Sciences
Chinese University of Hong Kong
Funding Information:

This work was supported by LKS Institute of Health Sciences and the Strategic Investment of the Chinese University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorAjonuma, LCen_HK
dc.contributor.authorChan, PKSen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorFok, KLen_HK
dc.contributor.authorWong, CHYen_HK
dc.contributor.authorTsang, LLen_HK
dc.contributor.authorTang, XXen_HK
dc.contributor.authorHo, LSen_HK
dc.contributor.authorLau, MCen_HK
dc.contributor.authorChung, CMen_HK
dc.contributor.authorHe, Qen_HK
dc.contributor.authorHuang, HYen_HK
dc.contributor.authorYang, DZen_HK
dc.contributor.authorRowlands, DKen_HK
dc.contributor.authorChung, YWen_HK
dc.contributor.authorChan, HCen_HK
dc.date.accessioned2010-05-31T03:23:10Z-
dc.date.available2010-05-31T03:23:10Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Obstetrics And Gynaecology Research, 2008, v. 34 n. 6, p. 923-930en_HK
dc.identifier.issn1341-8076en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58062-
dc.description.abstractBackground: Genital Chlamydia (C) trachomatis infection has been recognized as the single most common cause of pelvic inflammatory disease leading to severe tubal damage, ectopic pregnancy, infertility and hydrosalpinx. However, the mechanism underlying the formation of hydrosalpinx induced by C. trachomatis infection remains largely unknown. We performed this study to determine the involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel that regulates epithelial electrolyte and fluid secretion, in hydrosalpinx fluid formation. Methods: Western blot analysis was used to determine CFTR expression in the hydrosalpinges that were seen on the ultrasound scans of infertile assisted reproduction treatment patients. Correlation with C. trachomatis infection was done by testing patients' sera for C. trachomatis immunoglobulin G antibody titer using a Capita enzyme-linked immunosorbent assay based kit. CFTR involvement was further verified in a rat C. trachomatis infection model and confirmed using CFTR mutant (CFTR tm1Unc) mice. Results: Here we report on the up-regulated expression of CFTR in the hydrosalpinx tissues of infertile patients with detectable serum levels of C. trachomatis antibody (immunoglobulin G). In a rat model, increased CFTR expression and fluid accumulation could be observed in the uterine horns infected with C. trachomatis elementary bodies, which was reversed by antibiotics treatment. In C. trachomatis-infected CFTR tm1Unc mice, however, no detectable fluid accumulation was observed. Conclusion: These findings suggest the involvement of CFTR in the pathogenesis of hydrosalpinx fluid formation and may provide grounds for a better treatment strategy to improve assisted reproduction treatment outcome in infertile patients with hydrosalpinx. © 2008 Japan Society of Obstetrics and Gynecology.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Obstetrics and Gynaecology Researchen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectCFTRen_HK
dc.subjectChlamydia trachomatisen_HK
dc.subjectHydrosalpinx fluiden_HK
dc.subjectInfectionen_HK
dc.subjectInfertilityen_HK
dc.subject.meshChlamydia Infections - metabolism - microbiology-
dc.subject.meshChlamydia trachomatis - isolation and purification-
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - metabolism-
dc.subject.meshFallopian Tube Diseases - metabolism - microbiology-
dc.subject.meshAntibodies, Bacterial/blood-
dc.titleInvolvement of cystic fibrosis transmembrane conductance regulator (CFTR) in the pathogenesis of hydrosalpinx induced by Chlamydia trachomatis infectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1341-8076&volume=34&issue=6&spage=923&epage=930&date=2008&atitle=Involvement+of+cystic+fibrosis+transmembrane+conductance+regulator+(CFTR)+in+the+pathogenesis+of+hydrosalpinx+induced+by+Chlamydia+trachomatis+infectionen_HK
dc.identifier.emailAjonuma, LC: louisca@hkucc.hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hku.hken_HK
dc.identifier.authorityAjonuma, LC=rp00051en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1447-0756.2008.00826.xen_HK
dc.identifier.pmid19012687-
dc.identifier.scopuseid_2-s2.0-55649104483en_HK
dc.identifier.hkuros161526en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55649104483&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue6en_HK
dc.identifier.spage923en_HK
dc.identifier.epage930en_HK
dc.identifier.isiWOS:000260544600001-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridAjonuma, LC=6602292557en_HK
dc.identifier.scopusauthoridChan, PKS=7403497792en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridFok, KL=15030063000en_HK
dc.identifier.scopusauthoridWong, CHY=36192460900en_HK
dc.identifier.scopusauthoridTsang, LL=7103290955en_HK
dc.identifier.scopusauthoridTang, XX=24169711800en_HK
dc.identifier.scopusauthoridHo, LS=7402955607en_HK
dc.identifier.scopusauthoridLau, MC=24169129500en_HK
dc.identifier.scopusauthoridChung, CM=25123914000en_HK
dc.identifier.scopusauthoridHe, Q=36652577800en_HK
dc.identifier.scopusauthoridHuang, HY=37025365800en_HK
dc.identifier.scopusauthoridYang, DZ=14319464000en_HK
dc.identifier.scopusauthoridRowlands, DK=7103109123en_HK
dc.identifier.scopusauthoridChung, YW=7404388001en_HK
dc.identifier.scopusauthoridChan, HC=7403402737en_HK
dc.identifier.citeulike3476832-

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