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Article: Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis

TitleUpregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
Authors
Issue Date2007
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 2007, v. 60 n. 5, p. 510-514 How to Cite?
AbstractBackground: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. Aim: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. Methods: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. Results: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p = 0.022). Conclusions: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.
Persistent Identifierhttp://hdl.handle.net/10722/57292
ISSN
2014 Impact Factor: 2.915
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, HFen_HK
dc.contributor.authorChan, YPen_HK
dc.contributor.authorWong, MLYen_HK
dc.contributor.authorKwok, WKen_HK
dc.contributor.authorChan, KKen_HK
dc.contributor.authorLee, PYen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorLaw, SYKen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorChan, KWen_HK
dc.date.accessioned2010-04-12T01:32:08Z-
dc.date.available2010-04-12T01:32:08Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Clinical Pathology, 2007, v. 60 n. 5, p. 510-514en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57292-
dc.description.abstractBackground: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. Aim: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. Methods: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. Results: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p = 0.022). Conclusions: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.en_HK
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCarcinoma, Squamous Cell - metabolism - secondary - surgeryen_HK
dc.subject.meshEsophageal Neoplasms - metabolism - pathology - surgeryen_HK
dc.subject.meshUp-Regulationen_HK
dc.subject.meshTumor Markers, Biological - biosynthesis - geneticsen_HK
dc.subject.meshNuclear Proteins - biosynthesis - geneticsen_HK
dc.titleUpregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=60&issue=5&spage=510&epage=514&date=2007&atitle=Upregulation+of+Twist+in+oesophageal+squamous+cell+carcinoma+is+associated+with+neoplastic+transformation+and+distant+metastasisen_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_HK
dc.identifier.emailLaw, SYK: slaw@hku.hken_HK
dc.identifier.emailWong, YC: ycwong@hkucc.hku.hken_HK
dc.identifier.emailChan, KW: hrmtckw@hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityLaw, SYK=rp00437en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/jcp.2006.039099en_HK
dc.identifier.pmid16822877en_HK
dc.identifier.pmcidPMC1994533-
dc.identifier.scopuseid_2-s2.0-34249866557en_HK
dc.identifier.hkuros125360-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249866557&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue5en_HK
dc.identifier.spage510en_HK
dc.identifier.epage514en_HK
dc.identifier.isiWOS:000246594100012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYuen, HF=14018633400en_HK
dc.identifier.scopusauthoridChan, YP=14009821700en_HK
dc.identifier.scopusauthoridWong, MLY=37021112700en_HK
dc.identifier.scopusauthoridKwok, WK=14825201200en_HK
dc.identifier.scopusauthoridChan, KK=8986914100en_HK
dc.identifier.scopusauthoridLee, PY=37056356400en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridLaw, SYK=7202241293en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK

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