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Article: Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
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TitleUpregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
 
AuthorsYuen, HF1
Chan, YP1
Wong, MLY1
Kwok, WK1
Chan, KK1
Lee, PY1
Srivastava, G1
Law, SYK1
Wong, YC1
Wang, X1
Chan, KW2 1
 
Issue Date2007
 
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
CitationJournal Of Clinical Pathology, 2007, v. 60 n. 5, p. 510-514 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2006.039099
 
AbstractBackground: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. Aim: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. Methods: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. Results: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p = 0.022). Conclusions: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.
 
ISSN0021-9746
2013 Impact Factor: 2.551
 
DOIhttp://dx.doi.org/10.1136/jcp.2006.039099
 
PubMed Central IDPMC1994533
 
ISI Accession Number IDWOS:000246594100012
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYuen, HF
 
dc.contributor.authorChan, YP
 
dc.contributor.authorWong, MLY
 
dc.contributor.authorKwok, WK
 
dc.contributor.authorChan, KK
 
dc.contributor.authorLee, PY
 
dc.contributor.authorSrivastava, G
 
dc.contributor.authorLaw, SYK
 
dc.contributor.authorWong, YC
 
dc.contributor.authorWang, X
 
dc.contributor.authorChan, KW
 
dc.date.accessioned2010-04-12T01:32:08Z
 
dc.date.available2010-04-12T01:32:08Z
 
dc.date.issued2007
 
dc.description.abstractBackground: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. Aim: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. Methods: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. Results: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p = 0.022). Conclusions: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationJournal Of Clinical Pathology, 2007, v. 60 n. 5, p. 510-514 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2006.039099
 
dc.identifier.doihttp://dx.doi.org/10.1136/jcp.2006.039099
 
dc.identifier.epage514
 
dc.identifier.hkuros125360
 
dc.identifier.isiWOS:000246594100012
 
dc.identifier.issn0021-9746
2013 Impact Factor: 2.551
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC1994533
 
dc.identifier.pmid16822877
 
dc.identifier.scopuseid_2-s2.0-34249866557
 
dc.identifier.spage510
 
dc.identifier.urihttp://hdl.handle.net/10722/57292
 
dc.identifier.volume60
 
dc.languageeng
 
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Clinical Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCarcinoma, Squamous Cell - metabolism - secondary - surgery
 
dc.subject.meshEsophageal Neoplasms - metabolism - pathology - surgery
 
dc.subject.meshUp-Regulation
 
dc.subject.meshTumor Markers, Biological - biosynthesis - genetics
 
dc.subject.meshNuclear Proteins - biosynthesis - genetics
 
dc.titleUpregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong