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Article: Parallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon cancer

TitleParallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon cancer
Authors
Issue Date2007
PublisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/
Citation
Molecular Biology Of The Cell, 2007, v. 18 n. 11, p. 4245-4260 How to Cite?
AbstractPosttranslational mechanisms are implicated in the development of epithelial cell polarity, but little is known about the patterns of gene expression and transcriptional regulation during this process. We characterized temporal patterns of gene expression during cell-cell adhesion-initiated polarization of cultured human Caco-2 cells, which develop structural and functional polarity resembling enterocytes in vivo. A distinctive switch in gene expression patterns occurred upon formation of cell-cell contacts. Comparison to gene expression patterns in normal human colon and colon tumors revealed that the pattern in proliferating, nonpolarized Caco-2 cells paralleled patterns seen in human colon cancer in vivo, including expression of genes involved in cell proliferation. The pattern switched in polarized Caco-2 cells to one more closely resembling that in normal colon tissue, indicating that regulation of transcription underlying Caco-2 cell polarization is similar to that during enterocyte differentiation in vivo. Surprisingly, the temporal program of gene expression in polarizing Caco-2 cells involved changes in signaling pathways (e.g., Wnt, Hh, BMP, FGF) in patterns similar to those during migration and differentiation of intestinal epithelial cells in vivo, despite the absence of morphogen gradients and interactions with stromal cells characteristic of enterocyte differentiation in situ. The full data set is available at http://microarray-pubs.stanford.edu/CACO2. © 2007 by The American Society for Cell Biology.
Persistent Identifierhttp://hdl.handle.net/10722/57152
ISSN
2015 Impact Factor: 4.037
2015 SCImago Journal Rankings: 3.665
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSääf, AMen_HK
dc.contributor.authorHalbleib, JMen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorSiu, TYen_HK
dc.contributor.authorSuet, YLen_HK
dc.contributor.authorNelson, WJen_HK
dc.contributor.authorBrown, POen_HK
dc.date.accessioned2010-04-12T01:27:26Z-
dc.date.available2010-04-12T01:27:26Z-
dc.date.issued2007en_HK
dc.identifier.citationMolecular Biology Of The Cell, 2007, v. 18 n. 11, p. 4245-4260en_HK
dc.identifier.issn1059-1524en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57152-
dc.description.abstractPosttranslational mechanisms are implicated in the development of epithelial cell polarity, but little is known about the patterns of gene expression and transcriptional regulation during this process. We characterized temporal patterns of gene expression during cell-cell adhesion-initiated polarization of cultured human Caco-2 cells, which develop structural and functional polarity resembling enterocytes in vivo. A distinctive switch in gene expression patterns occurred upon formation of cell-cell contacts. Comparison to gene expression patterns in normal human colon and colon tumors revealed that the pattern in proliferating, nonpolarized Caco-2 cells paralleled patterns seen in human colon cancer in vivo, including expression of genes involved in cell proliferation. The pattern switched in polarized Caco-2 cells to one more closely resembling that in normal colon tissue, indicating that regulation of transcription underlying Caco-2 cell polarization is similar to that during enterocyte differentiation in vivo. Surprisingly, the temporal program of gene expression in polarizing Caco-2 cells involved changes in signaling pathways (e.g., Wnt, Hh, BMP, FGF) in patterns similar to those during migration and differentiation of intestinal epithelial cells in vivo, despite the absence of morphogen gradients and interactions with stromal cells characteristic of enterocyte differentiation in situ. The full data set is available at http://microarray-pubs.stanford.edu/CACO2. © 2007 by The American Society for Cell Biology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/en_HK
dc.relation.ispartofMolecular Biology of the Cellen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Biology of the Cell. Copyright © American Society for Cell Biology.en_HK
dc.subject.meshCell Polarity - geneticsen_HK
dc.subject.meshColon - drug effects - metabolismen_HK
dc.subject.meshColonic Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshGene Expression Regulation - drug effects - geneticsen_HK
dc.subject.meshHealthen_HK
dc.titleParallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1059-1524&volume=18&issue=11&spage=4245&epage=4260&date=2007&atitle=Parallels+between+global+transcriptional+programs+of+polarizing+Caco-2+intestinal+epithelial+cells+in+vitro+and+gene+expression+programs+in+normal+colon+and+colon+canceren_HK
dc.identifier.emailSuet, YL:suetyi@hkucc.hku.hken_HK
dc.identifier.authoritySuet, YL=rp00359en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1091/mbc.E07-04-0309en_HK
dc.identifier.pmid17699589-
dc.identifier.pmcidPMC2043540en_HK
dc.identifier.scopuseid_2-s2.0-35848935648en_HK
dc.identifier.hkuros138951-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35848935648&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue11en_HK
dc.identifier.spage4245en_HK
dc.identifier.epage4260en_HK
dc.identifier.isiWOS:000250740000005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike1726480-

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