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Article: Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA

TitleHuman TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA
Authors
Issue Date2007
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2007, v. 282 n. 24, p. 17649-17657 How to Cite?
AbstractMammalian Dicer interacts with double-stranded RNA-binding protein TRBP or PACT to mediate RNA interference and micro-RNA processing. TRBP and PACT are structurally related but exert opposite regulatory activities on PKR. It is not understood whether TRBP and PACT are simultaneously required for Dicer. Here we show that TRBP directly interacts with PACT in vitro and in mammalian cells. TRBP and PACT form a triple complex with Dicer and facilitate the production of small interfering RNA (siRNA) by Dicer. Knockdown of both TRBP and PACT in cultured cells leads to significant inhibition of gene silencing mediated by short hairpin RNA but not by siRNA, suggesting that TRBP and PACT function primarily at the step of siRNA production. Taken together, these findings indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Our work significantly alters the current model for the assembly and function of the Dicer-containing complex that generates siRNA and micro-RNA in human. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/54240
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKok, KHen_HK
dc.contributor.authorNg, MHJen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2009-04-03T07:40:47Z-
dc.date.available2009-04-03T07:40:47Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2007, v. 282 n. 24, p. 17649-17657en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54240-
dc.description.abstractMammalian Dicer interacts with double-stranded RNA-binding protein TRBP or PACT to mediate RNA interference and micro-RNA processing. TRBP and PACT are structurally related but exert opposite regulatory activities on PKR. It is not understood whether TRBP and PACT are simultaneously required for Dicer. Here we show that TRBP directly interacts with PACT in vitro and in mammalian cells. TRBP and PACT form a triple complex with Dicer and facilitate the production of small interfering RNA (siRNA) by Dicer. Knockdown of both TRBP and PACT in cultured cells leads to significant inhibition of gene silencing mediated by short hairpin RNA but not by siRNA, suggesting that TRBP and PACT function primarily at the step of siRNA production. Taken together, these findings indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Our work significantly alters the current model for the assembly and function of the Dicer-containing complex that generates siRNA and micro-RNA in human. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.subject.meshFluorescence Resonance Energy Transferen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteins - genetics - metabolismen_HK
dc.subject.meshMultiprotein Complexesen_HK
dc.subject.meshNucleic Acid Conformationen_HK
dc.subject.meshRNA, Small Interfering - genetics - metabolismen_HK
dc.titleHuman TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNAen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=282&issue=24&spage=17649&epage=17657&date=2007&atitle=Human+TRBP+and+PACT+directly+interact+with+each+other+and+associated+with+dicer+to+facilitate+the+production+of+small+interfering+RNA+en_HK
dc.identifier.emailKok, KH:khkok@hku.hken_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityKok, KH=rp01455en_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1074/jbc.M611768200en_HK
dc.identifier.pmid17452327-
dc.identifier.scopuseid_2-s2.0-34547115237en_HK
dc.identifier.hkuros127318-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547115237&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume282en_HK
dc.identifier.issue24en_HK
dc.identifier.spage17649en_HK
dc.identifier.epage17657en_HK
dc.identifier.eissn1083-351X-
dc.identifier.isiWOS:000247084500037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001082916-
dc.identifier.scopusauthoridKok, KH=7006862631en_HK
dc.identifier.scopusauthoridNg, MHJ=27067825500en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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