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Article: Differential cytokine expression in EBV positive peripheral T cell lymphomas

TitleDifferential cytokine expression in EBV positive peripheral T cell lymphomas
Authors
KeywordsCytokines
Epstein-Barr virus
Peripheral T cell lymphomas
Issue Date1999
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 1999, v. 52 n. 5, p. 269-274 How to Cite?
AbstractAim - To investigate whether specific cytokines are secreted locally at the tumour site in Epstein-Barr virus (EBV) positive peripheral T cell lymphoma (PTCL). Methods - An RNase protection assay system was used to study the differential expression of 21 cytokines in parallel in eight cases of EBV positive non-nasal PTCL, and compared with 11 EBV negative non-nasal PTCLs and three EBV positive nasal natural killer (NK) cell lymphomas. Results - Among the eight EBV positive cases, interferon γ (IFN-γ), lymphotoxin β (LTβ), interleukin 10 (IL-10), tumour necrosis factor a (TNF-α), transforming growth factor β1 (TGF-β1), and IL-1 receptor a (IL-Ra) were frequently detectable. IL-15, IL-6, IL-4, IL-1β, TNF-β, and IL-9 were sporadically detectable. Of the frequently detectable cytokines, IFN-γ and LTβ were commonly detected in the EBV negative cases. For cases with > 50% EBV encoded small non-polyadenylated RNA (EBER) positive cells, IL-10, TNF- α, and TGF-β1 were detected in three of three cases, and IL-1Ra in two of three cases. For cases with < 20% EBER positive cells, IL-10 was detected in three of five cases, TNF-α in two of four cases, but TGF-β1 and IL-1Ra were not detected. Interestingly, IL-6 was detected in two of three cases with > 50% EBER positive cells, but only in one of five cases with < 20% EBER positive cells. For comparison, in NK cell lymphomas, IL-10, TNF-α, IL-1Ra, and IL-6 were all detectable, but TGF-β was not detected at all. Immunohistochemical staining revealed IL-10 in many cells; in contrast, EBV latent membrane protein 1 (LMP1) was only found to be positive in isolated cells. Conclusions - Certain cytokines, such as IL-10 and TNF-α, might be expressed preferentially in EBV positive peripheral T cell lymphomas. It is likely that such a cytokine environment enhances EBV infection and contributes towards tumorigenesis.
Persistent Identifierhttp://hdl.handle.net/10722/53405
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, JWYen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2009-04-03T07:18:55Z-
dc.date.available2009-04-03T07:18:55Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 1999, v. 52 n. 5, p. 269-274en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/53405-
dc.description.abstractAim - To investigate whether specific cytokines are secreted locally at the tumour site in Epstein-Barr virus (EBV) positive peripheral T cell lymphoma (PTCL). Methods - An RNase protection assay system was used to study the differential expression of 21 cytokines in parallel in eight cases of EBV positive non-nasal PTCL, and compared with 11 EBV negative non-nasal PTCLs and three EBV positive nasal natural killer (NK) cell lymphomas. Results - Among the eight EBV positive cases, interferon γ (IFN-γ), lymphotoxin β (LTβ), interleukin 10 (IL-10), tumour necrosis factor a (TNF-α), transforming growth factor β1 (TGF-β1), and IL-1 receptor a (IL-Ra) were frequently detectable. IL-15, IL-6, IL-4, IL-1β, TNF-β, and IL-9 were sporadically detectable. Of the frequently detectable cytokines, IFN-γ and LTβ were commonly detected in the EBV negative cases. For cases with > 50% EBV encoded small non-polyadenylated RNA (EBER) positive cells, IL-10, TNF- α, and TGF-β1 were detected in three of three cases, and IL-1Ra in two of three cases. For cases with < 20% EBER positive cells, IL-10 was detected in three of five cases, TNF-α in two of four cases, but TGF-β1 and IL-1Ra were not detected. Interestingly, IL-6 was detected in two of three cases with > 50% EBER positive cells, but only in one of five cases with < 20% EBER positive cells. For comparison, in NK cell lymphomas, IL-10, TNF-α, IL-1Ra, and IL-6 were all detectable, but TGF-β was not detected at all. Immunohistochemical staining revealed IL-10 in many cells; in contrast, EBV latent membrane protein 1 (LMP1) was only found to be positive in isolated cells. Conclusions - Certain cytokines, such as IL-10 and TNF-α, might be expressed preferentially in EBV positive peripheral T cell lymphomas. It is likely that such a cytokine environment enhances EBV infection and contributes towards tumorigenesis.en_HK
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCytokinesen_HK
dc.subjectEpstein-Barr virusen_HK
dc.subjectPeripheral T cell lymphomasen_HK
dc.subject.meshCytokines - metabolismen_HK
dc.subject.meshEpstein-Barr Virus Infections - complications - immunologyen_HK
dc.subject.meshHerpesvirus 4, Human - isolation & purificationen_HK
dc.subject.meshLymphoma, T-Cell, Peripheral - immunology - virologyen_HK
dc.subject.meshTumor Necrosis Factor-alpha - metabolismen_HK
dc.titleDifferential cytokine expression in EBV positive peripheral T cell lymphomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=52&issue=5&spage=269&epage=274&date=1999&atitle=Differential+cytokine+expression+in+EBV+positive+peripheral+T+cell+lymphomasen_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10748876-
dc.identifier.pmcidPMC395709-
dc.identifier.scopuseid_2-s2.0-0343060572en_HK
dc.identifier.hkuros52623-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0343060572&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue5en_HK
dc.identifier.spage269en_HK
dc.identifier.epage274en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, JWY=7402649982en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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