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Article: Real-time PCR assay using molecular beacon for quantitation of hepatitis B virus DNA

TitleReal-time PCR assay using molecular beacon for quantitation of hepatitis B virus DNA
Authors
Issue Date2004
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2004, v. 42 n. 8, p. 3438-3440 How to Cite?
AbstractLevels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and limited dynamic range. The aim of this study was to develop a sensitive and accurate assay for measuring HBV genomic DNA using real-time PCR with a molecular beacon (HBV beacon assay). The performance of this assay was validated by testing serial dilutions of the two EUROHEP HBV DNA standards (ad and ay subtypes) of known concentrations. The assay showed low intra-assay (<7%) and interassay (<5%) variations for both subtypes. Its dynamic range was found to be 101 to 107 copies per reaction (1.0 x 102 to 109 copies ml-1). The assay was further evaluated clinically using serum samples from 175 individuals with chronic hepatitis B. The HBV DNA level measured by this assay showed good correlation with that measured by the commercially available COBAS AMPLICOR HBV Monitor test (r = 0.901; P < 0.001). The higher sensitivity and broader dynamic range of this assay compared to the existing commercial assays will provide an ideal tool for monitoring disease progression and treatment efficacy in HBV-infected patients, in particular for those with low levels of HBV viremia.
Persistent Identifierhttp://hdl.handle.net/10722/49138
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSum, SSMen_HK
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorYuan, HJen_HK
dc.contributor.authorYu, Jen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorHo, Den_HK
dc.contributor.authorZhang, Len_HK
dc.date.accessioned2008-06-12T06:35:14Z-
dc.date.available2008-06-12T06:35:14Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2004, v. 42 n. 8, p. 3438-3440en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49138-
dc.description.abstractLevels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and limited dynamic range. The aim of this study was to develop a sensitive and accurate assay for measuring HBV genomic DNA using real-time PCR with a molecular beacon (HBV beacon assay). The performance of this assay was validated by testing serial dilutions of the two EUROHEP HBV DNA standards (ad and ay subtypes) of known concentrations. The assay showed low intra-assay (<7%) and interassay (<5%) variations for both subtypes. Its dynamic range was found to be 101 to 107 copies per reaction (1.0 x 102 to 109 copies ml-1). The assay was further evaluated clinically using serum samples from 175 individuals with chronic hepatitis B. The HBV DNA level measured by this assay showed good correlation with that measured by the commercially available COBAS AMPLICOR HBV Monitor test (r = 0.901; P < 0.001). The higher sensitivity and broader dynamic range of this assay compared to the existing commercial assays will provide an ideal tool for monitoring disease progression and treatment efficacy in HBV-infected patients, in particular for those with low levels of HBV viremia.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2004, v. 42 n. 8, p. 3438-3440en_HK
dc.subject.meshHepatitis B virus - genetics - isolation & purificationen_HK
dc.subject.meshHepatitis B, Chronic - blood - diagnosisen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshBacteriophage M13 - geneticsen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.titleReal-time PCR assay using molecular beacon for quantitation of hepatitis B virus DNAen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=42&issue=8&spage=3438&epage=3440&date=2004&atitle=Real-time+PCR+assay+using+molecular+beacon+for+quantitation+of+hepatitis+B+virus+DNAen_HK
dc.identifier.emailWong, DKH:danywong@hku.hken_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.42.8.3438-3440.2004en_HK
dc.identifier.pmid15297480-
dc.identifier.pmcidPMC497581en_HK
dc.identifier.scopuseid_2-s2.0-3843116668en_HK
dc.identifier.hkuros97712-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3843116668&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue8en_HK
dc.identifier.spage3438en_HK
dc.identifier.epage3440en_HK
dc.identifier.isiWOS:000223286500009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSum, SSM=6603889128en_HK
dc.identifier.scopusauthoridWong, DKH=7401535819en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridYuan, HJ=7402446707en_HK
dc.identifier.scopusauthoridYu, J=7405525088en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridHo, D=7402971998en_HK
dc.identifier.scopusauthoridZhang, L=8783285300en_HK

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