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Article: Comparison of the CMV Brite Turbo assay and the Digene Hybrid Capture CMV DNA (version 2.0) assay for quantitation of cytomegalovirus in renal transplant recipients

TitleComparison of the CMV Brite Turbo assay and the Digene Hybrid Capture CMV DNA (version 2.0) assay for quantitation of cytomegalovirus in renal transplant recipients
Authors
Issue Date2000
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2000, v. 38 n. 10, p. 3743-3745 How to Cite?
AbstractWe compared the CMV Brite Turbo Kit (BT) and the Digene Hybrid Capture CMV DNA (version 2.0) assay (HC2) in the quantitation of pp65 antigenemia and cytomegalovirus (CMV) DNA levels in immunosuppressed renal transplant recipients. Of 123 blood specimens collected from 24 renal transplant recipients, BT and HC2 assays detected 35 and 39 positive samples, respectively. The overall concordance rate between the two assays was 90%. Discordant results were observed at low levels of viremia, so that 8 samples were HC2 positive but BT negative and another 4 were BT positive but HC2 negative. There was good correlation (R 2 = 0.766; P < 0.01) between the levels of CMV DNA and pp65 antigenemia in the 31 concordant positive samples. Correlation between results obtained with the two assays was confirmed by longitudinal studies for a patient who developed clinical CMV disease. HC2 may be more sensitive at low viremia levels and allow earlier detection of impending CMV disease. The BT assay offered the advantage of a rapid (2-h) turnaround time. We conclude that BT and HC2 assays have similar sensitivity and efficacy in the diagnosis and monitoring of CMV infection and disease in renal transplant recipients. While the HC2 assay would be appropriate for centers that handle a large number of samples, the BT test may be more suitable for small sample numbers or when results are needed urgently.
Persistent Identifierhttp://hdl.handle.net/10722/49109
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, SKNen_HK
dc.contributor.authorLi, FKen_HK
dc.contributor.authorLai, KNen_HK
dc.contributor.authorChan, TMen_HK
dc.date.accessioned2008-06-12T06:34:37Z-
dc.date.available2008-06-12T06:34:37Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2000, v. 38 n. 10, p. 3743-3745en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49109-
dc.description.abstractWe compared the CMV Brite Turbo Kit (BT) and the Digene Hybrid Capture CMV DNA (version 2.0) assay (HC2) in the quantitation of pp65 antigenemia and cytomegalovirus (CMV) DNA levels in immunosuppressed renal transplant recipients. Of 123 blood specimens collected from 24 renal transplant recipients, BT and HC2 assays detected 35 and 39 positive samples, respectively. The overall concordance rate between the two assays was 90%. Discordant results were observed at low levels of viremia, so that 8 samples were HC2 positive but BT negative and another 4 were BT positive but HC2 negative. There was good correlation (R 2 = 0.766; P < 0.01) between the levels of CMV DNA and pp65 antigenemia in the 31 concordant positive samples. Correlation between results obtained with the two assays was confirmed by longitudinal studies for a patient who developed clinical CMV disease. HC2 may be more sensitive at low viremia levels and allow earlier detection of impending CMV disease. The BT assay offered the advantage of a rapid (2-h) turnaround time. We conclude that BT and HC2 assays have similar sensitivity and efficacy in the diagnosis and monitoring of CMV infection and disease in renal transplant recipients. While the HC2 assay would be appropriate for centers that handle a large number of samples, the BT test may be more suitable for small sample numbers or when results are needed urgently.en_HK
dc.format.extent390 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2000, v. 38 n. 10, p. 3743-3745en_HK
dc.subject.meshAntigens, Viral - blooden_HK
dc.subject.meshCytomegalovirus - genetics - isolation & purificationen_HK
dc.subject.meshCytomegalovirus Infections - diagnosisen_HK
dc.subject.meshDNA, Viral - blooden_HK
dc.subject.meshKidney Transplantationen_HK
dc.titleComparison of the CMV Brite Turbo assay and the Digene Hybrid Capture CMV DNA (version 2.0) assay for quantitation of cytomegalovirus in renal transplant recipientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=38&issue=10&spage=3743&epage=3745&date=2000&atitle=Comparison+of+the+CMV+brite+turbo+assay+and+the+Digene+Hybrid+Capture+CMV+DNA+(Version+2.0)+assay+for+quantitation+of+cytomegalovirus+in+renal+transplant+recipientsen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid11015394-
dc.identifier.pmcidPMC87467en_HK
dc.identifier.scopuseid_2-s2.0-0033775540en_HK
dc.identifier.hkuros61552-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033775540&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3743en_HK
dc.identifier.epage3745en_HK
dc.identifier.isiWOS:000089707600037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, SKN=36839065300en_HK
dc.identifier.scopusauthoridLi, FK=55210612400en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK

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